LOCUS       sequence-34439-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP08 (3N3K)
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggttccgcgt ggtagtccaa cagtaactcc aacagttaat cgggaaaaca
      121 agccaacatg ttatcctaaa gctgagatct caaggctttc tgcttctcag attcggaacc
      181 tcaatcctgt ttttggaggt tctggaccag ctcttactgg acttcgtaac ttaggaaata
      241 cttgttatat gaactcaata ttgcagtgcc tatgtaacgc tccacatttg gctgattatt
      301 tcaaccgaaa ctgttatcag gatgatatta acaggtcaaa tttgttgggg cataaaggtg
      361 aagtggcaga agaatttggt ataatcatga aagccctgtg gacaggacag tatagatata
      421 tcagtccaaa ggactttaaa atcaccattg ggaagatcaa tgaccagttt gcaggataca
      481 gtcagcaaga ttcacaagaa ttgcttctgt tcctaatgga tggtctccat gaagatctaa
      541 ataaagctga taatcggaag agatataaag aagaaaataa tgatcatctc gatgacttta
      601 aagctgcaga acatgcctgg cagaaacaca agcagctcaa tgagtctatt attgttgcac
      661 tttttcaggg tcaattcaaa tctacagtac agtgcctcac atgtcacaaa aagtctagga
      721 catttgaggc cttcatgtat ttgtctctac cactagcatc cacaagtaaa tgtaca
//
