LOCUS       sequence-34440-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-HSP90 (3N72)
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agaaataatt ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gcaataaaca aaaaccatta tggatgagaa
      121 aaccagaaga agttacaaat gaagaatatg caagcttcta taaatcatta acaaatgatt
      181 gggaagacca tttagctgtt aaacatttct ctgttgaagg acaattagaa tttaaagcct
      241 tattatttat accaaaaaga gcaccttttg atatgttcga aaatagaaaa aaaagaaata
      301 atatcaaatt atatgtaaga agagttttta ttatggatga ttgtgaagaa attattccag
      361 aatggttaaa ttttgttaag ggtgttgtcg attcagaaga tttaccactt aatatttcaa
      421 gagaatcatt acaacaaaat aaaatactta aggttatcaa aaaaaacctt atcaaaaaat
      481 gtttagacat gttctcagaa ttagctgaaa ataaggaaaa ctacaaaaag ttttatgaac
      541 aattcagcaa aaacttaaag ttgggtatcc acgaggataa cgcaaatcgt acaaagatca
      601 ccgaattact ccgattccaa acctcaaaat caggagacga aatgatcgga ttaaaagaat
      661 acgtagacag aatgaaggaa aaccaaaagg atatttacta tatcaccggt gaatccatca
      721 atgctgtttc taattctcca ttcttagaag ctttgaccaa aaaaggattc gaagttattt
      781 atatggttga t
//
