LOCUS       sequence-34441-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-MAPK2 (3N9X)
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg catcatcatc atcatcacag cagcggcaga
       61 gaaaacttgt atttccaggg cataaaaaat gtgcatgtgc cagataatta cataattaag
      121 cacttaatag gaagagggtc gtatgggtat gtatatttag cttatgataa aaatacagaa
      181 aaaaatgttg caataaaaaa ggtaaatcga atgtttgaag atttaattga ttgtaaaaga
      241 atattaagag aaataacaat tttgaataga ctaaaaagtg attatattat aagattatat
      301 gatttaataa tacctgatga tttattaaaa tttgacgaat tgtatatagt attagaaata
      361 gccgattcgg acttaaaaaa attatttaaa accccaatat ttttgacaga agaacatata
      421 aaaactatat tatataattt attattaggt gaaaatttta ttcacgaatc aggtattatc
      481 catagagatt taaaaccagc taactgttta ttaaatcaag attgctctgt taaagtatgt
      541 gattttggtt tagctagaac gataaatagt gaaaaagata ctaatatagt aaatgattta
      601 gaagaaaatg aagaaccagg gccacataat aaaaatttaa aaaaacaatt aactagccat
      661 gtagttacta gatggtatag agcccctgaa ttaatcctgt tacaagaaaa ttatactaaa
      721 tctatagata tatggtctac tggttgtatt tttgcagaac tattaaatat gttacaaagc
      781 catattaatg atcccactaa tagatttcct ttatttccag gatcttcttg ttttccacta
      841 tc
//
