LOCUS       sequence-34443-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CYP11A1 (3N9Y)
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(147..152,153..170,171..177)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    185..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(256..261,262..277,278..284)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    292..308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(351..356,357..372,373..379)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    387..403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc gatggtgtcc
       61 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
      121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      181 ggaattgtga gcggataaca atttcacaca ggaaacagga tcgatccatc gatgagctta
      241 ctccccatcc ccctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
      301 gataacaatt tcacacagga aacaggatca gcttactccc catccccctg ttgacaatta
      361 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      421 gatccatcga tgcttaggag gtcatatggc tagctcagaa gataaaataa cagtccactt
      481 tataaaccgt gatggtgaaa cattaacaac caaaggaaaa gttggtgatt ctctgctaga
      541 tgttgtggtt gaaaataatc tagatattga tggctttggt gcatgtgagg gaaccctggc
      601 ttgttcaacc tgtcacctca tctttgaaga tcacatatat gagaagttag atgcaatcac
      661 tgatgaggag aatgacatgc tcgatctggc atatggacta acagacagat cacggttggg
      721 ctgccaaatc tgtttgacaa aatctatgga caatatgact gttcgagtgc ctgaaacagt
      781 ggctgatg
//
