LOCUS       sequence-344440-        9682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a sfGFP dropout expression cassette in place of Part 1 of 
            HexaPro-D614G spike to be used in the Spike Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1 DO
FEATURES             Location/Qualifiers
     source          1..9682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             463..474
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     sig_peptide     complement(637..696)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     promoter        complement(742..760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(803..822)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          805..937
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(1056..1080)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1090..1108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1111..1129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(1131..1334)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1335..1714
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1886..1905
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1981..2084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2085..2153,2154..2945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2303..2322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3116..3704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3605..3624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3858..3875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3992..4013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4028..4033,4034..4051,4052..4058)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4066..4082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4071..4093
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4090..4106
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4090..4106
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4155..4276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4166..4185)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4220..4239
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4406..5428)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(4406..5428)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(5436..5483)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5615..5634
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5767..5991
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5980..5997
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(6044..6190)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(6233..6256)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(6263..6286)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(6293..6358)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(9299..9326)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(9342..9413)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(9425..9682,1..456))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gatgtaaaca ttgtggctgt taaaattgta ttccagctta tggcccagga tattgccgtc
       61 ctctttaaag tcaatgcctt tcagctcaat gcggtttacc agggtatcgc cttcaaattt
      121 cacttccgca cgcgttttgt acgtgccgtc atccttaaag gaaatcgtgc gttcctgcac
      181 atagccttcc ggcatggcgg acttgaagaa gtcatgctgc ttcatatggt ccggataacg
      241 agcaaagcac tgaacaccat aagtcagcgt cgttaccaga gtcggccaag gaaccggcag
      301 tttaccagta gtacagatga acttcagcgt cagtttacca ttagttgcgt caccttcacc
      361 ctcgccacgc acggaaaact tatgaccgtt gacatcacca tccagttcca ccagaatagg
      421 gacgacacca gtgaacagct cttcgccttt acgcatctag tatttctcct ctttctctag
      481 tagctaagat ccgaacctgc cgtttcttga gttgccgcga tgttaagaaa acattcataa
      541 attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt caaaatgacg
      601 catgaaatca cgtttcactt tccacctgcc accgtcacca gtggaacctg gaacccagag
      661 cagcagtacc catagcagga gtgtgtctgt ctccatcccg ggtcgactct agaggtacca
      721 cgcgtgaatt ctcgaggcta gcctatagtg agtcgtatta agtactctag ccttaagagc
      781 tgtaattgaa ctgggagtgg acacctgtgg agagaaaggc aaagtggatg tcagtaagac
      841 caataggtgc ctatcagaaa cgcaagagtc ttctctgtct cgacaagccc agtttctatt
      901 ggtctcctta aacctgtctt gtaaccttga tacttacctg cccagtgcct cacgaccaac
      961 ttctgcagct taagtttaaa cgctagagtc cggaggctgg atcggtcccg gtgtcttcta
     1021 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
     1081 cgtcgacgat ctctatcact gatagggaga tctctatcac tgatagggag agctctgctt
     1141 atatagacct cccaccgtac acgcctaccg cccatttgtg tcaatggggc ggagttgtta
     1201 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     1261 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     1321 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     1381 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     1441 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     1501 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     1561 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     1621 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     1681 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
     1741 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
     1801 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
     1861 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     1921 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgt
     1981 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2041 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2101 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2161 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2221 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2281 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2341 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2401 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2461 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2521 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2581 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2641 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2701 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2761 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2821 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2881 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2941 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3001 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3061 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc cgacttgagc
     3661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3781 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3841 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3901 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3961 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4021 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4081 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg
     4141 gtatacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4201 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4261 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     4321 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     4381 tggaatcgaa atctcgtagt acgtgctatt cctttgccct cggacgagtg ctggggcgtc
     4441 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     4501 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     4561 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     4621 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
     4681 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     4741 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     4801 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     4861 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     4921 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     4981 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     5041 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
     5101 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     5161 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
     5221 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     5281 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     5341 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     5401 cagacgtcgc ggtgagttca ggctttttgg ccaaggaagt tcctatactt tctagagaat
     5461 aggaacttcg gaataggaac ttctaggtac gtgaaccatc accctaatca agttttttgg
     5521 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5581 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5641 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     5701 atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct
     5761 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     5821 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     5881 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     5941 aaggcacggg ggagggacaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     6001 atcagcgggt ttaaacgggc cctctagact cgagcggccg ttaacgtggc ttcttctgcc
     6061 aaagcatgat gaggatgata agggagatga tggtgagcac caccagggcc aggatggctg
     6121 agatcaccac caccttaaag ggcaaggagt gtggcaccac gatgacctcc tgcgtgtcct
     6181 ggcccacagc agctgagccg ccagatccgc cccctccact accgccgccg cctgggccct
     6241 ggaacagcac ctccagggat cccttctcga actgggggtg ggaccaggcg ctcttgtcat
     6301 cgtcatcctt gtaatcgatg tcatgatctt tataatcacc gtcatggtct ttgtagtcgg
     6361 atctgcccag gaatgtgctc agcagtaccc actcgccgtc cttccgcacg taggcctggc
     6421 catctctggg ggcctcgggg atgtatccgg atccctgctc gtatttgccg agctcctgca
     6481 ggtcgatcag ggactcgttc aggttcttgg ccacctcgtt cagtctgtcg atttcctttt
     6541 ggatgttcac cacgctggcg ttaatgccac tgatgtcgcc caggtccaca tcggggctgg
     6601 tgtggttttt aaagtacttg tccagctctt ccttaaaaga gtccagctca ggttgcagag
     6661 ggtcgtacac tgtgttgttc acgattccga tgaccacgtc acaattgcca ctcacgaagg
     6721 tgttgtcggt ggtgataatc tggggttcgt agaagtttct ttgtgtcacg aaccagtgtg
     6781 tgccattgga aacgaaaacg ccctctcttg ggaagtgggc cttgccgtcg tggcagatgg
     6841 caggggcggt tgtgaaattc ttctcttggg caggcacgta tgtcacatgc aggaacacca
     6901 cgccgtgcgg cgcgctctgg gggaagctca tcaggtgata gcctttgccg cagaagtcaa
     6961 cgcgtttaga ctgtcccagc acacactcgg acatcttggt agcggcgaga ttggcgctgg
     7021 ctcttatttc agcggccctg atcagctgtt gggtcacgta ggtttgcagg gactgcagtc
     7081 tgcctgtgat cagtctatcg atctggactt cggcttctgg agggtccagt ctagacagga
     7141 tatcgttcag cacagagctg atggcgccaa aattgctgct gagctgcttc accagggtgt
     7201 tcagggcttg ggcgttctga ttcaccacgt cctgcagttt gcccagggcg ctgggggtag
     7261 aggagaggga gtcctggatc ttgccgatgg cagaattgaa ctggttagcg atcagcttct
     7321 ggttttcata cagcacgttc tgggtcacgc cgattccgtt gaatctatag gccatctgca
     7381 tgggaaaggg gatctgcagg gcggggccgg cgccaaatgt ccacccagat gtgatggtgc
     7441 cagccagcag ggcagaggtg tactgagcga tcatctcgtc ggtcagcaga ggtgggagaa
     7501 ctgtcaggcc gttgaatttc tgggcgcaga tcagatctct ggcggctatg tcgcccaggc
     7561 aatcgccata ctgtttgata aagccggcat cggccagggt gaccttgttg aacagcagat
     7621 cctcgatagg gctccgcttg ctaggcttag aaggatcagg gagaatctgg ctaaagttaa
     7681 agccgccgaa gtccttgata ggtggggtct tgtagatctg cttcacttgg gcgaacacct
     7741 cctgggtatt tttgtcctgt tccacggcga ttccggtcag cgcccggttc agttgggtgc
     7801 aaaaggagcc gtattgcagc agcaggttgc tgcattcagt gctgtcgcca cagatgtaca
     7861 ttgtacagtc cacgctggtc ttggtcatgc tcacgggcag gatctctgtt gtgacgctaa
     7921 ttgtaaagtt ggtgggaatg gcgatagagt tgttggaata ggccacgctg ttctctgcgc
     7981 ccaggctcat ggtgtaggcg atgatgctct ggctagctac agaggaagca gacccagggc
     8041 tgtttgtctg ggtctggtag ctggcacaga tgccggcgcc gatagggatg tcgcactcgt
     8101 aactgttatt cacgtgctcg gcgccgatca gacatccggc cctggtctgg aacacattgg
     8161 agccagtgga gtacactctc caagtaggtg tcagctgatc ggcgtggatg gccacaggaa
     8221 cctcggtgca gttaacacct tggtacagca cagccacctg gttgctggta ttggtcccgg
     8281 gggtgatcac agaaactcct ccgaaggagc agggtgtaat atccaggatt tccagggtct
     8341 gtggatctct gacggcatct gtggtgtcag cgatatcacg gccgaactgc tggaagggga
     8401 ggaacttttt gttgctttcg gtcagcacgc cggtcccggt caggccgttg aagttgaagt
     8461 tcacgcattt gttcttaacc agattggtgg acttcttagg accgcagacg gtggcggggg
     8521 cgtggagtaa ctcgaaggac agcacgacca cccggtatgg ctggtagccg actccattgg
     8581 taggctggaa gccgtagctt tgcagaggga agtaacagtt gaagccttcc accccgttac
     8641 aaggtgtaga accggcctgg tagatctctg tggagatgtc tctctcgaaa ggtttcaggt
     8701 tagacttgcg aaacagccgg tacaggtagt tgtagttgcc gcccaccttg ctatccaggt
     8761 tgttagagtt ccaggcgatc acgcagcctg taaaatcatc aggcagctta tagttgtagt
     8821 cagcgatctt accggtctgg cctggtgcga tctgccgcac ctcatcgcct ctaattacga
     8881 aggagtcggc gtacacgttg gtgaagcaca ggtcattcag ctttgttggg gaaacgccgt
     8941 agcacttgaa ggtgctgaaa gaggcgctgt tatacagcac ggagtagtcg gccacacaat
     9001 tgctgattct ctttctattc caggcgtaaa cggaggcaaa tcttgtggcg ttaaacacct
     9061 cgccgaaggg gcagaggttg gtaatgtttg gaaatcgcac gatgctctct gtaggctgca
     9121 cccggaagtt gctggtctgg tagatgccct tttccacggt gaaggacttc agggtgcact
     9181 ttgtctcaga gagagggtcc agggcgcagt cgacggcgtc tgtgattgtg ccgttctcat
     9241 tgtacttcag caggaaggtt ctaggttgca ggtagcccac aggtggcagg tgtataaacg
     9301 cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa
     9361 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     9421 gtcatttgta cagttcatcc ataccatgcg tgatgcccgc tgcggttacg aactccagca
     9481 gaaccatatg atcgcgtttc tcgttcggat ctttagacag aacgctttgc gtgctcagat
     9541 agtgattgtc tggcagcaga acaggaccat caccgattgg agtgttttgc tggtagtgat
     9601 cagccagctg cacgctgcca tcctccacgt tgtggcgaat tttaaaattc gctttaatgc
     9661 catttttttg tttatcggcg gt
//
