LOCUS       sequence-344445-        6613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses the extracellular and transmembrane domain of murine CD19 
            with a C terminal fusion of the LLO190 and SIINFEKL epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Veatch JR, Singhi N, Srivastava S, Szeto JL, Jesernig B, Stull SM, 
            Fitzgibbon M, Sarvothama M, Yechan-Gunja S, James SE, Riddell SR
  TITLE     A therapeutic cancer vaccine delivers antigens and adjuvants to 
            lymphoid tissues using genetically modified T cells.
  JOURNAL   J Clin Invest. 2021 Aug 16;131(16):e144195. doi: 10.1172/JCI144195.
  PUBMED    34396986
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2021-08-16"; volume: "131(16):e144195. doi"; pages:
            " 10.1172/JCI144195"
COMMENT     Sequence Label: pMP71-tCD19-LLO-SIINFEKL
FEATURES             Location/Qualifiers
     source          1..6613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2397..2800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             3306..3896
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3921..3937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3921..3937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3934..3956)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3945..3961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3969..3975,3976..3993,3994..3999))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4014..4035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4152..4169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4323..4911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4403..4422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5082..5873,5874..5942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5705..5724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5943..6047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6115..6133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6171..6193)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6293..6312
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6506..6528
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6520..6537
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6521..6537
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
       61 gttcctgccc cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga
      121 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
      181 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      241 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      301 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      361 ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca
      421 tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg actgcccacc
      481 tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc agggaccacc
      541 gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct ctgtctttgt
      601 gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttgg ctaactagat
      661 ctgtatctgg cggtcccgcg gaagaactga cgagttcgta ttcccggccg cagcccctgg
      721 gagacgtccc agcggcctcg ggggcccgtt ttgtggccca ttctgtatca gttaacctac
      781 ccgagtcgga ctttttggag ctccgccact gtccgagggg tacgtggctt tgttggggga
      841 cgagagacag agacacttcc cgcccccgtc tgaatttttg ctttcggttt tacgccgaaa
      901 ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
      961 tctgtatttg tctgaaaatt agctcgacaa agttaagtaa tagtccctct ctccaagctc
     1021 acttacaggc ggccgccatg ccatctcctc tccctgtctc cttcctcctc tttcttacct
     1081 tagtaggagg caggccccag aagtccttac tggtggaggt agaagaggga ggcaatgttg
     1141 tgctgccatg cctcccggac tcctcacctg tctcttctga gaagctggct tggtatcgag
     1201 gtaaccagtc aacacccttc ctggagctga gccccgggtc ccctggcctg ggattgcacg
     1261 tggggtccct gggcatcttg ctagtgattg tcaatgtctc agaccatatg gggggcttct
     1321 acctgtgcca gaagaggccc cctttcaagg acatctggca gcctgcctgg acagtgaacg
     1381 tggaggatag tggggagatg ttccggtgga atgcttcaga cgtcagggac ctggactgtg
     1441 acctaaggaa caggtcctct gggagccaca ggtccacttc tggttcccag ctgtatgtgt
     1501 gggctaaaga ccatcctaag gtctggggaa caaagcctgt atgtgcccct cgggggagca
     1561 gtttgaatca gagtctaatc aaccaagatc tcactgtggc acccggctcc acactttggc
     1621 tgtcctgtgg ggtaccccct gtcccagtgg ccaaaggctc catctcctgg acccatgtgc
     1681 atcctaggag acctaatgtt tcactactga gcctaagcct tgggggagag cacccggtca
     1741 gagagatgtg ggtttggggg tctcttctgc ttctgcccca agccacagct ttagatgaag
     1801 gcacctatta ttgtctccga ggaaacctga ccatcgagag gcacgtgaag gtcattgcaa
     1861 ggtcagtgtg gctctggctg ttgagaactg gtggatggat agtcccagtg gtgactttag
     1921 tatatgtcat cttctgtatg gtttctctgg tggcttttct ctattgtcaa agagccttta
     1981 tcctgagaag gaaaaggggt ggcagcggcg gtgctgtgaa tacactcgtc gaaagatgga
     2041 acgaaaagta tgcccaagct tatccaaacg tcagcgcaaa aattgattat gacgatgaaa
     2101 tgggaggatc tgggggtgag gtgtcaggac ttgaacaact tgaatccatt attaacttcg
     2161 agaaactcac tgaatggaca agctcaaatg tcatgtgaga attcgagcat cttaccgcca
     2221 tttattccca tatttgttct gtttttcttg atttgggtat acatttaaat gttaataaaa
     2281 caaaatggtg gggcaatcat ttacatttta tgggatatgt aattactagt tcaggtgtat
     2341 tgccacaaga caaacatgtt aagaaacttt cccgttattt acgctctgtt cctgttaatc
     2401 aacctctgga ttacaaaatt tgtgaaagat tgactgatat tcttaactat gttgctcctt
     2461 ttacgctgtg tggatatgct gctttaatgc ctctgtatca tgctattgct tcccgtacgg
     2521 ctttcgtttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2581 ccgttgtccg tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct
     2641 ggggcattgc caccacctgt caactccttt ctgggacttt cgctttcccc ctcccgatcg
     2701 ccacggcaga actcatcgcc gcctgccttg cccgctgctg gacaggggct aggttgctgg
     2761 gcactgataa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     2821 gtgttgccaa ctggatcctg cgcgggacgt ccttctgcta cgtcccttcg gctctcaatc
     2881 cagcggacct cccttcccga ggccttctgc cggttctgcg gcctctcccg cgtcttcgct
     2941 ttcggcctcc gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc
     3001 gtccggtccg tgttgcttgg tcgtcacctg tgcagaattg cgaaccatgg attccaccgt
     3061 gaactttgtc tcctggcatg caaatcgtca acttggcatg ccaagaataa ttcggatcca
     3121 agcttaggcc tgctcgcttt cttgctgtcc catttctatt aaaggttcct ttgttcccta
     3181 agtccaacta ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctag
     3241 cgctaagctt aacacgagcc atagatagaa taaaagattt tatttagtct ccagaaaaag
     3301 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     3361 aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga
     3421 gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     3481 gccaagaaca gttggaacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     3541 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     3601 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     3661 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     3721 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     3781 ccgggtaccc gtgttctcaa taaaccctct tgcagttgca tccgactcgt ggtctcgctg
     3841 ttccttggga gggtctcctc tgagtgattg actgcccacc tcgggggtct ttcattctcg
     3901 agcagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3961 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4021 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4081 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4141 tcttccgctc cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5101 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5161 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5221 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5281 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5341 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5401 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5461 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5521 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5581 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5641 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5701 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5761 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5821 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5881 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5941 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6001 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6061 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     6121 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6181 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     6241 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     6301 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     6361 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6421 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     6481 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     6541 ttagtactct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     6601 agagaagttc aga
//
