LOCUS       sequence-34445-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MLH1
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aattttgttt aactttaaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcatgt cgtttgtggc aggggttatt cggcggctgg
      121 acgagacagt ggtgaaccgc atcgcggcgg gggaagttat ccagcggcca gctaatgcta
      181 tcaaagagat gattgagaac tgtttagatg caaaatccac aagtattcaa gtgattgtta
      241 aagagggagg cctgaagttg attcagatcc aagacaatgg caccgggatc aggaaagaag
      301 atctggatat tgtatgtgaa aggttcacta ctagtaaact gcagtccttt gaggatttag
      361 ccagtatttc tacctatggc tttcgaggtg aggctttggc cagcataagc catgtggctc
      421 atgttactat tacaacgaaa acagctgatg gaaagtgtgc atacagagca agttactcag
      481 atggaaaact gaaagcccct cctaaaccat gtgctggcaa tcaagggacc cagatcacgg
      541 tggaggacct tttttacaac atagccacga ggagaaaagc tttaaaaaat ccaagtgaag
      601 aatatgggaa aattttggaa gttgttggca ggtattcagt acacaatgca ggcattagtt
      661 tctcagttaa aaaacaagga gagacagtag ctgatgttag gacactaccc aatgcctcaa
      721 ccgtggacaa tattcgctcc atctttggaa atgctgttag tcgagaactg atagaaat
//
