LOCUS       sequence-344452-        6859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses the extracellular and transmembrane domain of murine CD19 
            with a C terminal fusion of the LLO190 and minigenes of 3 
            neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Veatch JR, Singhi N, Srivastava S, Szeto JL, Jesernig B, Stull SM, 
            Fitzgibbon M, Sarvothama M, Yechan-Gunja S, James SE, Riddell SR
  TITLE     A therapeutic cancer vaccine delivers antigens and adjuvants to 
            lymphoid tissues using genetically modified T cells.
  JOURNAL   J Clin Invest. 2021 Aug 16;131(16):e144195. doi: 10.1172/JCI144195.
  PUBMED    34396986
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2021-08-16"; volume: "131(16):e144195. doi"; pages:
            " 10.1172/JCI144195"
COMMENT     Sequence Label: pMP71-tCD19-llo-mc38tmg
FEATURES             Location/Qualifiers
     source          1..6859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(147..163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(147..163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(160..182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(195..201,202..219,220..225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    240..261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(378..395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(549..1137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(629..648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1308..2099,2100..2168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1931..1950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2169..2273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2341..2359
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2397..2419)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2519..2538
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2732..2754
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2746..2763
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2747..2763
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5482..5885
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             join(6391..6859,1..122)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cgtcgcccgg gtacccgtgt tctcaataaa ccctcttgca gttgcatccg actcgtggtc
       61 tcgctgttcc ttgggagggt ctcctctgag tgattgactg cccacctcgg gggtctttca
      121 ttctcgagca gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      181 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      241 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      301 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      361 gggcgctctt ccgctccctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2281 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2341 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2401 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2461 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     2521 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     2581 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     2641 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     2701 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2761 agtgaattag tactctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
     2821 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
     2881 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacagt tggaacagca
     2941 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     3001 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
     3061 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     3121 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     3181 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
     3241 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
     3301 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
     3361 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
     3421 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
     3481 actagttggc taactagatc tgtatctggc ggtcccgcgg aagaactgac gagttcgtat
     3541 tcccggccgc agcccctggg agacgtccca gcggcctcgg gggcccgttt tgtggcccat
     3601 tctgtatcag ttaacctacc cgagtcggac tttttggagc tccgccactg tccgaggggt
     3661 acgtggcttt gttgggggac gagagacaga gacacttccc gcccccgtct gaatttttgc
     3721 tttcggtttt acgccgaaac cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     3781 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gctcgacaaa gttaagtaat
     3841 agtccctctc tccaagctca cttacaggcg gccgccatgc catctcctct ccctgtctcc
     3901 ttcctcctct ttcttacctt agtaggaggc aggccccaga agtccttact ggtggaggta
     3961 gaagagggag gcaatgttgt gctgccatgc ctcccggact cctcacctgt ctcttctgag
     4021 aagctggctt ggtatcgagg taaccagtca acacccttcc tggagctgag ccccgggtcc
     4081 cctggcctgg gattgcacgt ggggtccctg ggcatcttgc tagtgattgt caatgtctca
     4141 gaccatatgg ggggcttcta cctgtgccag aagaggcccc ctttcaagga catctggcag
     4201 cctgcctgga cagtgaacgt ggaggatagt ggggagatgt tccggtggaa tgcttcagac
     4261 gtcagggacc tggactgtga cctaaggaac aggtcctctg ggagccacag gtccacttct
     4321 ggttcccagc tgtatgtgtg ggctaaagac catcctaagg tctggggaac aaagcctgta
     4381 tgtgcccctc gggggagcag tttgaatcag agtctaatca accaagatct cactgtggca
     4441 cccggctcca cactttggct gtcctgtggg gtaccccctg tcccagtggc caaaggctcc
     4501 atctcctgga cccatgtgca tcctaggaga cctaatgttt cactactgag cctaagcctt
     4561 gggggagagc acccggtcag agagatgtgg gtttgggggt ctcttctgct tctgccccaa
     4621 gccacagctt tagatgaagg cacctattat tgtctccgag gaaacctgac catcgagagg
     4681 cacgtgaagg tcattgcaag gtcagtgtgg ctctggctgt tgagaactgg tggatggata
     4741 gtcccagtgg tgactttagt atatgtcatc ttctgtatgg tttctctggt ggcttttctc
     4801 tattgtcaaa gagcctttat cctgagaagg aaaaggggtg gcagcggcgg tgctgtgaat
     4861 acactcgtcg aaagatggaa cgaaaagtat gcccaagctt atccaaacgt cagcgcaaaa
     4921 attgattatg acgatgaaat gggaggatct gggggtatcc ctacaggcat ccccgtgcat
     4981 ctggaactgg ccagcatgac caacatggaa ctgatgagca gcatcgtgca ccagcaggtt
     5041 ttccctgctg tggctggtgg atctggcggc ggagtgaatg gaagagtgct ggaactgttc
     5101 agagccgctc agctggccaa cgatgtggtg ctgcagatta tggaactgtg cggcgccaca
     5161 agactcggcg gaggaagcgg aggacttgag gctggacaga gcctggtcat cagcgccagc
     5221 atcatcgtgt tcaacctgct cgagctggaa ggcgactaca gggacgacca catcttcagc
     5281 tgagaattcg agcatcttac cgccatttat tcccatattt gttctgtttt tcttgatttg
     5341 ggtatacatt taaatgttaa taaaacaaaa tggtggggca atcatttaca ttttatggga
     5401 tatgtaatta ctagttcagg tgtattgcca caagacaaac atgttaagaa actttcccgt
     5461 tatttacgct ctgttcctgt taatcaacct ctggattaca aaatttgtga aagattgact
     5521 gatattctta actatgttgc tccttttacg ctgtgtggat atgctgcttt aatgcctctg
     5581 tatcatgcta ttgcttcccg tacggctttc gttttctcct ccttgtataa atcctggttg
     5641 ctgtctcttt atgaggagtt gtggcccgtt gtccgtcaac gtggcgtggt gtgctctgtg
     5701 tttgctgacg caacccccac tggctggggc attgccacca cctgtcaact cctttctggg
     5761 actttcgctt tccccctccc gatcgccacg gcagaactca tcgccgcctg ccttgcccgc
     5821 tgctggacag gggctaggtt gctgggcact gataattccg tggtgttgtc ggggaagctg
     5881 acgtcctttc catggctgct cgcctgtgtt gccaactgga tcctgcgcgg gacgtccttc
     5941 tgctacgtcc cttcggctct caatccagcg gacctccctt cccgaggcct tctgccggtt
     6001 ctgcggcctc tcccgcgtct tcgctttcgg cctccgacga gtcggatctc cctttgggcc
     6061 gcctccccgc ctgtttcgcc tcggcgtccg gtccgtgttg cttggtcgtc acctgtgcag
     6121 aattgcgaac catggattcc accgtgaact ttgtctcctg gcatgcaaat cgtcaacttg
     6181 gcatgccaag aataattcgg atccaagctt aggcctgctc gctttcttgc tgtcccattt
     6241 ctattaaagg ttcctttgtt ccctaagtcc aactactaaa ctgggggata ttatgaaggg
     6301 ccttgagcat ctggattctg cctagcgcta agcttaacac gagccataga tagaataaaa
     6361 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     6421 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
     6481 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
     6541 taagcagttc ctgccccggc tcagggccaa gaacagttgg aacagcagaa tatgggccaa
     6601 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     6661 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     6721 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     6781 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
     6841 ccagtcctcc gatagactg
//
