LOCUS       sequence-34448-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KNS2
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg catcatcatc atcatcacag cagcggcaga
       61 gaaaacttgt atttccaggg cggcggctac gagatccccg cgcggctgcg gacgctccac
      121 aacctggtga tccagtacgc ctcgcagggg cgctacgagg tagctgtgcc cctctgcaag
      181 caggccctgg aggacctgga gaagacttca ggacacgacc acccggacgt ggccaccatg
      241 ctcaacatcc tggccttggt gtacagggat cagaataaat acaaagatgc agctaaccta
      301 ctgaatgatg ccttggctat tcgtgagaaa actttgggca aagatcatcc tgcggtggcg
      361 gcgactttga ataaccttgc agtcctttat ggtaaaagag ggaagtacaa agaagcagag
      421 ccgttgtgta aaagagctct ggaaatccga gaaaaggttt tggggaagga tcaccccgat
      481 gttgccaagc agttaaataa cttggcctta ctgtgccaga accagggcaa gtatgaagaa
      541 gtagaatatt attatcaaag agccctcgag atctaccaga caaaactggg acctgatgac
      601 cccaacgtgg ctaagacgaa aaataacctg gcatcctgct atttgaaaca aggaaagttc
      661 aagcaagcag aaacactgta caaagagatt ctcactcgtg cacatgaaag ggagtttggt
      721 tctgtagatg atgaaaataa acccatctgg atgcatgctg
//
