LOCUS       sequence-34449-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-AHA1
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(588..606)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      602..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tgagctttga aattacggaa gagtattatg
      121 ttccccctga ggttttattt aacgctttta ccgatgctta tacattaaca cgactatcca
      181 ggggatcttt agctgaagtg gatttaaaag ttggaggaaa gttcagcctt ttttctggga
      241 gcatattagg agaatttaca gaaataacaa agccacacaa aatagtcgag aaatggaaat
      301 ttagagactg gaatgaatgt gactacagta cagtaacagt ggaatttata agtgtaaaag
      361 aaaatcacac aaagttaaag ctcacacata ataatatacc agcaagcaat aaatataacg
      421 aaggtggtgt tttggaaagg tgcaaaaatg gatggacaca aaacttttta cataatatag
      481 aagttatatt aggatatccg aaaaagaaat gatgacgaag cttgcggccg cactcgagga
      541 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      601 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      661 aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
      721 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
      781 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
//
