LOCUS       sequence-34452-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     SGC Q2
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BAT8 (3RJW)
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtaatcgggc catccgcaca gagaagatca
      121 tctgccggga cgtggctcgg ggctatgaga acgtgcccat tccctgtgtc aacggtgtgg
      181 atggggagcc ctgccctgag gattacaagt acatctcaga gaactgcgag acgtccacca
      241 tgaacatcga tcgcaacatc acccacctgc agcactgcac gtgtgtggac gactgctcta
      301 gctccaactg cctgtgcggc cagctcagca tccggtgctg gtatgacaag gatgggcgat
      361 tgctccagga atttaacaag attgagcctc cgctgatttt cgagtgtaac caggcgtgct
      421 catgctggag aaactgcaag aaccgggtcg tacagagtgg catcaaggtg cggctacagc
      481 tctaccgaac agccaagatg ggctgggggg tccgcgccct gcagaccatc ccacagggga
      541 ccttcatctg cgagtatgtc ggggagctga tctctgatgc tgaggctgat gtgagagagg
      601 atgattctta cctcttcgac ttagacaaca aggatggaga ggtgtactgc atagatgccc
      661 gttactatgg caacatcagc cgcttcatca accacctgtg tgaccccaac atcattcccg
      721 tccgggtctt catgctgcac caagacctgc gatttccacg catcgccttc ttcagttccc
      781 gagacatccg gactggggag gagctagggt ttgactatgg cgaccgcttc t
//
