LOCUS       sequence-344529-       13397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puromycin resistant lentiviral vector for expression of PAK5 S573N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
  TITLE     Melanoma-associated mutants within the serine-rich domain of PAK5 
            direct kinase activity to mitogenic pathways.
  JOURNAL   Oncotarget. 2018 May 22;9(39):25386-25401. doi: 
            10.18632/oncotarget.25356. eCollection 2018 May 22.
  PUBMED    29875996
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.25356"; journalName: "Oncotarget"; date: 
            "2018-05-22- 22"; volume: "9"; issue: "39"; pages: "25386-25401"
COMMENT     Sequence Label: pLenti-3xFLAG-Puro PAK5 S573N
FEATURES             Location/Qualifiers
     source          1..13397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(493..1284,1285..1353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1116..1135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1354..1458)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1506..1640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             1808..2441
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2488..2613
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3110..3343
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3528..3572
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3870..3987
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4052..4426)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      4423..4432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4436..4454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4446..4493)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     5511..5530
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5690..5755
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7981..8554
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8149..8166)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8376..8395
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8557..9159
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9056..9076
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9175..9763
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9228..9248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9646..9657)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9970..10203
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10304..10321)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10310..10534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10580..11008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10667..10686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10877..10898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11017..11037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11022..11351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11202..11337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11264..11283
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12559..12680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12729..12745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12729..12745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12742..12764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12753..12769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12777..12783,12784..12801,12802..12807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12822..12843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12960..12977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(13131..13397,1..322))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13211..13230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       61 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     1501 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1561 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1621 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     1681 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     1741 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     1801 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     1861 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     1921 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     1981 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     2041 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     2101 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     2161 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     2221 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     2281 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2341 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2401 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2461 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2521 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2581 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2641 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2701 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2761 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     2821 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     2881 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     2941 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3001 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3061 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3121 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     3181 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3241 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3301 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3361 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3421 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3481 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3541 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3601 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3661 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3721 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3781 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     3841 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3901 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     3961 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     4021 tggttagtac cgggcccgct ctagtccgga atcagtcctg ctcctcggcc acgaagtgca
     4081 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     4141 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     4201 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     4261 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     4321 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     4381 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtg gccctcctat
     4441 agtgagtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
     4501 gcaggtccga ggttctaggc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     4561 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     4621 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     4681 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     4741 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     4801 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     4861 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     4921 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     4981 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     5041 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     5101 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     5161 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
     5221 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
     5281 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
     5341 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
     5401 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
     5461 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
     5521 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
     5581 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
     5641 taaattctgg ccgtttttgg cttttttgtt agacgctagc ggatccatgg actacaaaga
     5701 ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg acaagctagc
     5761 gatgtttggg aagaaaaaga aaaagattga aatatctggc ccgtccaact ttgaacacag
     5821 ggttcatact gggtttgatc cacaagagca gaagtttacc ggccttcccc agcagtggca
     5881 cagcctgtta gcagatacgg ccaacaggcc aaagcctatg gtggaccctt catgcatcac
     5941 acccatccag ctggctccta tgaagacaat cgttagagga aacaaaccct gcaaggaaac
     6001 ctccatcaac ggcctgctag aggattttga caacatctcg gtgactcgct ccaactccct
     6061 aaggaaagaa agcccaccca ccccagatca gggagcctcc agccacggtc caggccacgc
     6121 ggaagaaaat ggcttcatca ccttctccca gtattccagc gaatccgata ctactgctga
     6181 ctacacgacc gaaaagtaca gggagaagag tctctatgga gatgatctgg atccgtatta
     6241 tagaggcagc cacgcagcca agcaaaatgg gcacgtaatg aaaatgaagc acggggaggc
     6301 ctactattct gaggtgaagc ctttgaaatc cgattttgcc agattttctg ccgattatca
     6361 ctcacatttg gactcactga gcaaaccaag tgaatacagt gacctcaagt gggagtatca
     6421 gagagcctcg agtagctccc ctctggatta ttcattccaa ttcacacctt ctagaactgc
     6481 agggaccagc gggtgctcca aggagagcct ggcgtacagt gaaagtgaat ggggacccag
     6541 cctggatgac tatgacagga ggccaaagtc ttcgtacctg aatcagacaa gccctcagcc
     6601 caccatgcgg cagaggtcca ggtcaggctc gggactccag gaaccgatga tgccatttgg
     6661 agcaagtgca tttaaaaccc atccccaagg acactcctac aactcctaca cctaccctcg
     6721 cttgtccgag cccacaatgt gcattccaaa ggtggattac gatcgagcac agatggtcct
     6781 cagccctcca ctgtcagggt ctgacaccta ccccaggggc cctgccaaac tacctcaaag
     6841 tcaaagcaaa tcgggctatt cctcaagcag tcaccagtac ccgtctgggt accacaaagc
     6901 caccttgtac catcacccct ccctgcagag cagttcgcag tacatctcca cggcttccta
     6961 cctgagctcc ctcagcctct catccagcac ctacccgccg cccagctggg gctcctcctc
     7021 cgaccagcag ccctccaggg tgtcccatga acagtttcgg gcggccctgc agctggtggt
     7081 cagcccagga gaccccaggg aatacttggc caactttatc aaaatcgggg aaggctcaac
     7141 cggcatcgta tgcatcgcca ccgagaaaca cacagggaaa caagttgcag tgaagaaaat
     7201 ggacctccgg aagcaacaga gacgagaact gcttttcaat gaggtcgtga tcatgcggga
     7261 ttaccaccat gacaatgtgg ttgacatgta cagcagctac cttgtcggcg atgagctctg
     7321 ggtggtcatg gagtttctag aaggtggtgc cttgacagac attgtgactc acaccagaat
     7381 gaatgaagaa cagatagcta ctgtctgcct gtcagttctg agagctctct cctaccttca
     7441 taaccaagga gtgattcaca gggacataaa aagtgacaac atcctcctga caagcgatgg
     7501 ccggataaag ttgtctgatt ttggtttctg tgctcaagtt tccaaagagg tgccgaagag
     7561 gaaatcattg gttggcactc cctactggat ggcccctgag gtgatttcta ggctacctta
     7621 tgggacagag gtggacatct ggtccctcgg gatcatggtg atagaaatga ttgatggcga
     7681 gcccccctac ttcaatgagc ctcccctcca ggcgatgcgg aggatccggg acagtttacc
     7741 tccaagagtg aaggacctac acaaggtttc ttcagtgctc cggggattcc tagacttgat
     7801 gttggtgagg gagccctctc agagagcaac agcccaggaa ctcctcggac atccattctt
     7861 aaaactagca ggtccaccgt cttgcattgt ccccctcatg agacaataca ggcatcactg
     7921 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagttaac gcaaattccg
     7981 cccctctccc tccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     8041 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     8101 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     8161 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     8221 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     8281 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     8341 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     8401 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     8461 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     8521 gggacgtggt tttcctttga aaaacacgat aataccatgg ccaccgagta caagcccacg
     8581 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     8641 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     8701 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8761 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8821 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8881 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8941 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     9001 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac
     9061 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     9121 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag ttcgcgtctg gaacaatcaa
     9181 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9241 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9301 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9361 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9421 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9481 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9541 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     9601 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9661 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9721 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     9781 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     9841 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     9901 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     9961 agaggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10021 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10081 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10141 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10201 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    10261 cagagagtga gaggccttga cattgtttaa acccgctgat cagcctcgac tgtgccttct
    10321 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    10381 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    10441 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    10501 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    10561 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    10621 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    10681 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10741 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10801 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10861 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10921 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10981 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    11041 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    11101 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    11161 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    11221 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    11281 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    11341 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga
    11401 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
    11461 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
    11521 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
    11581 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
    11641 gggaattaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
    11701 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
    11761 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
    11821 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    11881 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    11941 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
    12001 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    12061 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
    12121 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    12181 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    12241 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    12301 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    12361 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    12421 aaggaatagc acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    12481 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    12541 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    12601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    12661 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    12721 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    12781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    12841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    12901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    12961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    13021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    13081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    13141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    13201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    13261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    13321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    13381 tgtgtgcacg aaccccc
//
