LOCUS       sequence-344530-       13397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puromycin resistant lentiviral vector for expression of PAK5 
            K478R/479R S364L; PAK7 Kinase dead.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
  TITLE     Melanoma-associated mutants within the serine-rich domain of PAK5 
            direct kinase activity to mitogenic pathways.
  JOURNAL   Oncotarget. 2018 May 22;9(39):25386-25401. doi: 
            10.18632/oncotarget.25356. eCollection 2018 May 22.
  PUBMED    29875996
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.25356"; journalName: "Oncotarget"; date: 
            "2018-05-22- 22"; volume: "9"; issue: "39"; pages: "25386-25401"
COMMENT     Sequence Label: pLenti-3xFLAG-Puro PAK5 K478R/479R S364L
FEATURES             Location/Qualifiers
     source          1..13397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    631..756
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1253..1486
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1671..1715
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2013..2130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2195..2569)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2566..2575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2579..2597)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2589..2636)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     3654..3673
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3833..3898
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6124..6697
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6292..6309)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6519..6538
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6700..7302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7199..7219
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7318..7906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7371..7391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7789..7800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8113..8346
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8447..8464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8453..8677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8723..9151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8810..8829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9020..9041
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9160..9180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9165..9494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9345..9480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9407..9426
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10702..10823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10872..10888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10872..10888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10885..10907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10896..10912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10920..10926,10927..10944,10945..10950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10965..10986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11103..11120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11274..11862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11354..11373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12033..12824,12825..12893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12656..12675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12894..12998)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13046..13180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             join(13348..13397,1..584)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
       61 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
      121 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
      181 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
      241 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
      301 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
      361 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
      421 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      481 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      541 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      601 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      661 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      721 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      781 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
      841 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
      901 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
      961 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1021 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1081 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1141 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1201 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1261 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1321 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1381 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1441 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1501 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1561 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1621 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1681 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1741 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1801 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1861 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1921 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1981 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2041 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2101 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2161 gtttggttag taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt
     2221 gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga
     2281 tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact
     2341 cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca
     2401 cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt
     2461 cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg
     2521 cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc
     2581 tatagtgagt cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt
     2641 gctgcaggtc cgaggttcta ggctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     2701 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     2761 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     2821 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     2881 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     2941 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     3001 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     3061 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     3121 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     3181 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     3241 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     3301 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     3361 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     3421 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     3481 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     3541 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     3601 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     3661 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     3721 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     3781 cgctaaattc tggccgtttt tggctttttt gttagacgct agcggatcca tggactacaa
     3841 agaccatgac ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagct
     3901 agcgatgttt gggaagaaaa agaaaaagat tgaaatatct ggcccgtcca actttgaaca
     3961 cagggttcat actgggtttg atccacaaga gcagaagttt accggccttc cccagcagtg
     4021 gcacagcctg ttagcagata cggccaacag gccaaagcct atggtggacc cttcatgcat
     4081 cacacccatc cagctggctc ctatgaagac aatcgttaga ggaaacaaac cctgcaagga
     4141 aacctccatc aacggcctgc tagaggattt tgacaacatc tcggtgactc gctccaactc
     4201 cctaaggaaa gaaagcccac ccaccccaga tcagggagcc tccagccacg gtccaggcca
     4261 cgcggaagaa aatggcttca tcaccttctc ccagtattcc agcgaatccg atactactgc
     4321 tgactacacg accgaaaagt acagggagaa gagtctctat ggagatgatc tggatccgta
     4381 ttatagaggc agccacgcag ccaagcaaaa tgggcacgta atgaaaatga agcacgggga
     4441 ggcctactat tctgaggtga agcctttgaa atccgatttt gccagatttt ctgccgatta
     4501 tcactcacat ttggactcac tgagcaaacc aagtgaatac agtgacctca agtgggagta
     4561 tcagagagcc tcgagtagct cccctctgga ttattcattc caattcacac cttctagaac
     4621 tgcagggacc agcgggtgct ccaaggagag cctggcgtac agtgaaagtg aatggggacc
     4681 cagcctggat gactatgaca ggaggccaaa gtcttcgtac ctgaatcaga caagccctca
     4741 gcccaccatg cggcagaggt ccaggtcagg ctcgggactc caggaaccga tgatgccatt
     4801 tggagcaagt gcatttaaaa cccatcccca aggacactcc tacaactcct acacctaccc
     4861 tcgcttgtcc gagcccacaa tgtgcattcc aaaggtggat tacgatcgag cacagatggt
     4921 cctcagccct ccactgtcag ggtctgacac ctaccccagg ggccctgcca aactacctca
     4981 aagtcaaagc aaattgggct attcctcaag cagtcaccag tacccgtctg ggtaccacaa
     5041 agccaccttg taccatcacc cctccctgca gagcagttcg cagtacatct ccacggcttc
     5101 ctacctgagc tccctcagcc tctcatccag cacctacccg ccgcccagct ggggctcctc
     5161 ctccgaccag cagccctcca gggtgtccca tgaacagttt cgggcggccc tgcagctggt
     5221 ggtcagccca ggagacccca gggaatactt ggccaacttt atcaaaatcg gggaaggctc
     5281 aaccggcatc gtatgcatcg ccaccgagaa acacacaggg aaacaagttg cagtgaggag
     5341 aatggacctc cggaagcaac agagacgaga actgcttttc aatgaggtcg tgatcatgcg
     5401 ggattaccac catgacaatg tggttgacat gtacagcagc taccttgtcg gcgatgagct
     5461 ctgggtggtc atggagtttc tagaaggtgg tgccttgaca gacattgtga ctcacaccag
     5521 aatgaatgaa gaacagatag ctactgtctg cctgtcagtt ctgagagctc tctcctacct
     5581 tcataaccaa ggagtgattc acagggacat aaaaagtgac tccatcctcc tgacaagcga
     5641 tggccggata aagttgtctg attttggttt ctgtgctcaa gtttccaaag aggtgccgaa
     5701 gaggaaatca ttggttggca ctccctactg gatggcccct gaggtgattt ctaggctacc
     5761 ttatgggaca gaggtggaca tctggtccct cgggatcatg gtgatagaaa tgattgatgg
     5821 cgagcccccc tacttcaatg agcctcccct ccaggcgatg cggaggatcc gggacagttt
     5881 acctccaaga gtgaaggacc tacacaaggt ttcttcagtg ctccggggat tcctagactt
     5941 gatgttggtg agggagccct ctcagagagc aacagcccag gaactcctcg gacatccatt
     6001 cttaaaacta gcaggtccac cgtcttgcat tgtccccctc atgagacaat acaggcatca
     6061 ctgaattctg cagatatcca gcacagtggc ggccgctcga gtctagagtt aacgcaaatt
     6121 ccgcccctct ccctcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     6181 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     6241 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     6301 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     6361 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     6421 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6481 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6541 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6601 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     6661 acggggacgt ggttttcctt tgaaaaacac gataatacca tggccaccga gtacaagccc
     6721 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     6781 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     6841 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6901 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6961 gtgttcgccg agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7021 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7081 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7141 ggagtggagg cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc
     7201 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7261 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagttcgcgt ctggaacaat
     7321 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7381 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7441 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7501 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7561 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7621 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7681 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7861 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     7921 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     7981 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     8041 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8101 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     8161 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8221 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8281 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8341 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8401 tatcagagag tgagaggcct tgacattgtt taaacccgct gatcagcctc gactgtgcct
     8461 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     8521 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     8581 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     8641 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     8701 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8761 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8821 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8881 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8941 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9001 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9061 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9121 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     9181 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9241 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9301 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     9361 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9421 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9481 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     9541 tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     9601 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     9661 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     9721 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     9781 ttggggaatt aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     9841 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     9901 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     9961 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
    10021 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
    10081 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
    10141 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
    10201 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
    10261 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
    10321 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
    10381 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
    10441 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
    10501 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
    10561 gcaaaggaat agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
    10621 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10681 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    10741 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10801 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    10861 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    11041 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    11101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    12001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    12061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    12121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    12181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    12241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    12361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    13021 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    13081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13141 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    13201 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    13261 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    13321 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
    13381 atatccttga tctgtgg
//
