LOCUS       sequence-344534-        2246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAPTURE method 15A origin of replication receiver plasmid .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin 
            TA, Zhao H
  TITLE     Cas12a-assisted precise targeted cloning using in vivo Cre-lox 
            recombination.
  JOURNAL   Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4.
  PUBMED    33608525
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-19"; volume: "12(1):1171. doi"; pages: " 
            10.1038/s41467-021-21275-4"
COMMENT     Sequence Label: pBE44
FEATURES             Location/Qualifiers
     source          1..2246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..72
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    347..380
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        403..507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(508..576,577..1368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(726..745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1639..2184
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2085..2104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
       61 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
      121 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
      181 aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac
      241 cccgtcagta gctgaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      301 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaatataa cttcgtataa
      361 tgtatgctat acgaagttat tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      721 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      841 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      901 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      961 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1021 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1081 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1141 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1201 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1261 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1321 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1381 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1441 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1501 tgagcgtcag accccgtaga aaagatcaaa ggatcttcac aacttatatc gtatggggct
     1561 gacttcaggt gctacatttg aagagataaa ttgcactgaa atctagaaat attttatctg
     1621 attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     1681 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     1741 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     1801 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     1861 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     1921 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     1981 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     2041 aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     2101 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     2161 tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa
     2221 gtatcttcct ggcatcttcc aggaaa
//
