LOCUS       sequence-344537-        6162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAPTURE method BAC origin of replication receiver plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin 
            TA, Zhao H
  TITLE     Cas12a-assisted precise targeted cloning using in vivo Cre-lox 
            recombination.
  JOURNAL   Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4.
  PUBMED    33608525
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-19"; volume: "12(1):1171. doi"; pages: " 
            10.1038/s41467-021-21275-4"
COMMENT     Sequence Label: pBE48
FEATURES             Location/Qualifiers
     source          1..6162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(184..1155)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1155..2330)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    2656..2906
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2909..3664)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      3755..3974
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    4891..4924
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4947..5051
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5052..5120,5121..5912)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5270..5289)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agtctgatta
       61 tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc cagttatgct
      121 ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta aaacgtggtc
      181 gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct ctatacactc
      241 agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat acaatactgt
      301 cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag atgacgtttt
      361 aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc cagctttttt
      421 ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg taaaggcttt
      481 ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac
      541 tgatttaggc aatttggcgg tgttgataca gcgggtaata atcttacgtg aaatattttc
      601 cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc ttgcataacg
      661 ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg ataatgcagc
      721 catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg taagtgcagc
      781 agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc ttcgaccgaa
      841 cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca gtgcgtcctc
      901 agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct tctcaacact
      961 atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag gcaaagtaat
     1021 ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca tcggggcagc
     1081 tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg agcgtatgtt ttggaataac
     1141 aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc gaaaatttca
     1201 ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga agagcgttga
     1261 tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac ttcatccgtt
     1321 tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg ctcctccatc
     1381 cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg tacatcaggc
     1441 tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa aaactgcagt
     1501 gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc agcagcacat
     1561 acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat gacatcatag
     1621 tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt gggcagttta
     1681 ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag acaggaagga
     1741 ataatgtcaa gccccggcca gcaagtgggc tttattgcat aagtgacatc gtccttttcc
     1801 ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg tacccatccg
     1861 tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc
     1921 ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac gccaccttta
     1981 tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa tcgcgtacca
     2041 aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg ctcaacccgt
     2101 cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc tctgatagcc
     2161 tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg ggttattttc
     2221 ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt catttcatga
     2281 ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat cctttaatca
     2341 ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac aaaatcgcaa
     2401 agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa ggagataaga
     2461 agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg taaccgagca
     2521 tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc tcttacgctc
     2581 agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac acgcggatag
     2641 ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga actaaccccc
     2701 gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa actgccctca
     2761 aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg caggctgaag
     2821 gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg acgaaccacc
     2881 ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg atatcgcgga
     2941 aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat gagaggcgca
     3001 ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg cggaagtcag
     3061 gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg attttcagag
     3121 agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat aaacgcatgg
     3181 catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac cggttctgta
     3241 acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc cctctggatg
     3301 gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca tcgccggcat
     3361 cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat gcctgccgta
     3421 tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta gcaacatgga
     3481 tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc tggtcaacga
     3541 acagatacag catacgtttt tgatcccggg agagactata tgccgcctca gtgaggtcgt
     3601 ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc gctgtttccg
     3661 ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa ataaaaaaga
     3721 gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag
     3781 ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt cacactgaaa
     3841 gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta caaggcgctc
     3901 taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc cgtggataag
     3961 tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat ataagtgctg
     4021 ttccctggtg cttcctcgct cactcgaggg cttcgccctg tcgctcgact gcggcgagca
     4081 ctactggctg taaaaggaca gaccacatca tggttctgtg ttcattaggt tgttctgtcc
     4141 attgctgaca taatccgctc cacttcaacg taacaccgca cgaagatttc tattgttcct
     4201 gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca tcatgacaga acactacttc
     4261 ctataaacgc tacacaggct cctgagatta ataatgcgga tctctacgat aatgggagat
     4321 tttcccgact gtttcgttcg cttctcagtg gataacagcc agcttctctg tttaacagac
     4381 aaaaacagca tatccactca gttccacatt tccatataaa ggccaaggca tttattctca
     4441 ggataattgt ttcagcatcg caaccgcatc agactccggc atcgcaaact gcacccggtg
     4501 ccgggcagcc acatccagcg caaaaacctt cgtgtagact tccgttgaac tgatggactt
     4561 atgtcccatc aggctttgca gaactttcag cggtataccg gcatacagca tgtgcatcgc
     4621 ataggaatgg cggaacgtat gtggtgtgac cggaacagag aacgtcacac cgtcagcagc
     4681 agcggcggca accgcctccc caatccaggt cctgaccgtt ctgtccgtca cttcccagat
     4741 ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc tccatgagct tatcgcgaat
     4801 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
     4861 ccgggaagcc ctgggccaac ttttggcgaa ataacttcgt ataatgtatg ctatacgaag
     4921 ttattggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4981 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5041 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5101 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5161 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5221 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5281 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5341 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5401 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5461 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5521 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5581 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5641 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5701 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5761 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5821 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5881 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5941 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggcgagc tcgcgagctc
     6001 gtcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca cggcctgggt
     6061 aaccaggtat tttgtccaca taaccgtgcg caaaatgttg tggataagca ggacacagca
     6121 gcaatccaca gcaggcatac aaccgcacac cgaggttact cc
//
