LOCUS       sequence-344538-        9216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FnCas12a expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Enghiad B, Huang C, Guo F, Jiang G, Wang B, Tabatabaei SK, Martin 
            TA, Zhao H
  TITLE     Cas12a-assisted precise targeted cloning using in vivo Cre-lox 
            recombination.
  JOURNAL   Nat Commun. 2021 Feb 19;12(1):1171. doi: 10.1038/s41467-021-21275-4.
  PUBMED    33608525
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-19"; volume: "12(1):1171. doi"; pages: " 
            10.1038/s41467-021-21275-4"
COMMENT     Sequence Label: pET28-FnCas12a-TEV
FEATURES             Location/Qualifiers
     source          1..9216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     921..940
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(960..1037)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1243..1262
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1346..1365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1365..1389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1404..1426
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1448..1465
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1487..1507
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5426..5443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5496..5514)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5510..5557
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5594..6049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5681..5700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5891..5912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6141..6956)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6141..6956)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6864..6883
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6948..6967
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7078..7666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7567..7586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7820..7837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7852..7991
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7917..7936)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8077..8099
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8093..8284)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9093..9216,1..959))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
       61 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
      121 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
      181 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
      241 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
      301 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
      361 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
      421 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
      481 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
      541 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
      601 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
      661 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
      721 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
      781 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
      841 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
      901 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
      961 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     1021 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     1081 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     1141 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     1201 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     1261 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     1321 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     1381 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgtcctc
     1441 gtactaccat caccatcacc atcacgatta cgatatccca acgaccgaaa acctgtattt
     1501 tcagggcgcc atggatatga gcatctatca ggagttcgtg aataagtaca gcctgtccaa
     1561 gaccctgcgg tttgagctga tcccccaggg caagacactg gagaacatca aggccagggg
     1621 cctgatcctg gacgatgaga agcgcgccaa ggactataag aaggccaagc agatcatcga
     1681 taagtaccac cagttcttta tcgaggagat cctgagcagc gtgtgcatct ctgaggatct
     1741 gctgcagaat tacagcgacg tgtatttcaa gctgaagaag tctgacgatg acaacctgca
     1801 gaaggacttc aagagcgcca aggacaccat caagaagcag atcagcgagt atatcaagga
     1861 ctccgagaag tttaagaatc tgttcaacca gaatctgatc gatgccaaga agggccagga
     1921 gtccgacctg atcctgtggc tgaagcagtc taaggacaat ggcatcgagc tgttcaaggc
     1981 caactctgat atcaccgata tcgacgaggc cctggagatc atcaagagct ttaagggctg
     2041 gaccacatac tttaagggct tccacgagaa caggaagaac gtgtacagca gcaacgacat
     2101 ccctacaagc atcatctacc gcatcgtgga tgacaatctg ccaaagttcc tggagaacaa
     2161 ggccaagtat gagtccctga aggacaaggc ccccgaggcc atcaattacg agcagatcaa
     2221 gaaggatctg gccgaggagc tgaccttcga tatcgactat aagacatccg aggtgaacca
     2281 gcgggtgttt tctctggacg aggtgtttga gatcgccaat ttcaacaatt acctgaacca
     2341 gtccggcatc accaagttca atacaatcat cggcggcaag tttgtgaacg gcgagaatac
     2401 caagagaaag ggcatcaacg agtacatcaa tctgtatagc cagcagatca acgacaagac
     2461 cctgaagaag tacaagatga gcgtgctgtt caagcagatc ctgtccgata cagagtctaa
     2521 gagctttgtg atcgataagc tggaggatga ctctgacgtg gtgaccacaa tgcagagctt
     2581 ttatgagcag atcgccgcct tcaagaccgt ggaggagaag tctatcaagg agacactgag
     2641 cctgctgttc gatgacctga aggcccagaa gctggacctg tctaagatct acttcaagaa
     2701 cgataagtcc ctgaccgacc tgtctcagca ggtgtttgat gactatagcg tgatcggcac
     2761 cgccgtgctg gagtacatca cacagcagat cgccccaaag aacctggata atccctctaa
     2821 gaaggagcag gagctgatcg ccaagaagac cgagaaggcc aagtatctga gcctggagac
     2881 aatcaagctg gccctggagg agttcaataa gcaccgggat atcgacaagc agtgcagatt
     2941 tgaggagatc ctggccaact tcgccgccat ccccatgatc tttgatgaga tcgcccagaa
     3001 caaggacaat ctggcccaga tctccatcaa gtaccagaac cagggcaaga aggacctgct
     3061 gcaggcctct gccgaggatg acgtgaaggc catcaaggat ctgctggacc agaccaacaa
     3121 tctgctgcac aagctgaaga tcttccacat ctcccagtct gaggataagg ccaatatcct
     3181 ggataaggac gagcactttt atctggtgtt cgaggagtgt tacttcgagc tggccaacat
     3241 cgtgcccctg tacaacaaga tcagaaatta tatcacacag aagccttact ccgacgagaa
     3301 gtttaagctg aacttcgaga acagcaccct ggccaacggc tgggataaga ataaggagcc
     3361 tgacaacaca gccatcctgt tcatcaagga tgacaagtac tatctgggcg tgatgaataa
     3421 gaagaacaat aagatcttcg atgacaaggc catcaaggag aacaagggcg agggctacaa
     3481 gaagatcgtg tataagctgc tgcccggcgc caataagatg ctgcctaagg tgttcttttc
     3541 cgccaagtct atcaagttct acaacccatc cgaggacatc ctgcggatca gaaatcactc
     3601 cacccacaca aagaacggct ctccccagaa gggctatgag aagtttgagt tcaatatcga
     3661 ggattgccgg aagtttatcg acttctacaa gcagagcatc tccaagcacc ctgagtggaa
     3721 ggattttggc ttcaggttta gcgacaccca gcggtacaac tccatcgacg agttctacag
     3781 agaggtggag aatcagggct ataagctgac atttgagaac atctctgaga gctacatcga
     3841 cagcgtggtg aatcagggca agctgtacct gttccagatc tataacaagg acttcagcgc
     3901 ctattccaag ggccggccaa acctgcacac cctgtactgg aaggccctgt tcgatgagag
     3961 aaatctgcag gacgtggtgt ataagctgaa cggcgaggcc gagctgtttt acaggaagca
     4021 gtccatccct aagaagatca cacacccagc caaggaggcc atcgccaaca agaataagga
     4081 caatcctaag aaggagagcg tgttcgagta cgatctgatc aaggacaagc ggttcaccga
     4141 ggataagttc tttttccact gtccaatcac aatcaacttc aagtcctctg gcgccaacaa
     4201 gtttaatgac gagatcaatc tgctgctgaa ggagaaggcc aacgatgtgc acatcctgag
     4261 catcgaccgg ggcgagagac acctggccta ctataccctg gtggatggca agggcaatat
     4321 catcaagcag gataccttca acatcatcgg caatgacagg atgaagacaa actaccacga
     4381 taagctggcc gccatcgaga aggataggga ctccgcccgc aaggactgga agaagatcaa
     4441 caatatcaag gagatgaagg agggctatct gtctcaggtg gtgcacgaga tcgccaagct
     4501 ggtcatcgag tacaatgcca tcgtggtgtt cgaggatctg aacttcggct ttaagagggg
     4561 ccgctttaag gtggagaagc aggtgtatca gaagctggag aagatgctga tcgagaagct
     4621 gaattacctg gtgtttaagg ataacgagtt cgacaagacc ggaggcgtgc tgagggcata
     4681 ccagctgacc gccccctttg agacattcaa gaagatgggc aagcagacag gcatcatcta
     4741 ctatgtgcca gccggcttca cctccaagat ctgccccgtg acaggctttg tgaaccagct
     4801 gtaccctaag tatgagtccg tgtctaagag ccaggagttt ttcagcaagt tcgataagat
     4861 ctgttataat ctggacaagg gctacttcga gttttccttc gattataaga actttggcga
     4921 caaggccgcc aagggcaagt ggaccatcgc ctctttcggc agccggctga tcaactttag
     4981 aaattccgat aagaaccaca attgggacac ccgggaggtg tacccaacaa aggagctgga
     5041 gaagctgctg aaggactaca gcatcgagta tggccacggc gagtgcatca aggccgccat
     5101 ctgtggcgag agcgataaga agtttttcgc caagctgacc tccgtgctga atacaatcct
     5161 gcagatgcgg aacagcaaga ccggcacaga gctggactac ctgatctccc ccgtggccga
     5221 tgtgaacggc aacttcttcg acagcagaca ggcccccaag aatatgcctc aggatgccga
     5281 cgccaacggc gcctatcaca tcggcctgaa gggcctgatg ctgctgggca ggatcaagaa
     5341 caatcaggag ggcaagaagc tgaacctggt catcaagaac gaggagtact ttgagttcgt
     5401 gcagaaccgc aacaattgac tcgagcacca ccaccaccac cactgagatc cggctgctaa
     5461 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     5521 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     5581 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5701 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6001 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     6061 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6121 atccgctcat gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     6181 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     6241 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     6301 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     6361 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     6421 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     6481 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     6541 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     6601 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     6661 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     6721 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     6781 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     6841 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     6901 atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca
     6961 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta
     7021 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7201 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7261 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7321 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7381 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7441 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7501 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7561 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7621 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7681 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7741 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7801 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     7861 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     7921 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     7981 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8041 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8101 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     8161 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     8221 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     8281 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     8341 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     8401 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     8461 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     8521 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
     8581 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     8641 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     8701 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggggccgcca
     8761 tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg
     8821 cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc
     8881 tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga
     8941 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
     9001 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     9061 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9121 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9181 ggcgccaggg tggtttttct tttcaccagt gagacg
//
