LOCUS       sequence-34457-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   O'Connor CM, Lai CK, Collins K
  TITLE     Two purified domains of telomerase reverse transcriptase 
            reconstitute sequence-specific interactions with RNA.
  JOURNAL   J Biol Chem. 2005 Apr 29. 280(17):17533-9.
  PUBMED    15731105
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 29. 280(17):17533-9."
COMMENT     Sequence Label: pET28HistevTtTERT(1-516)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcgaaaacc tgtacttcca gggccatatg cagaaaatca acaacatcaa
      121 caataacaaa cagatgctga ctcgtaaaga agacctgctc accgttctga aacagatttc
      181 tgctctgaaa tacgtatcca acctgtacga gttcctgctt gctaccgaga aaatcgttca
      241 gacctccgaa ctggacaccc agttccagga atttctgacc actaccatta tcgcttccga
      301 acagaacctg gttgaaaact acaaacagaa atacaaccag ccaaacttct ctcaactgac
      361 catcaaacag gttatcgacg attctatcat tcttctgggt aacaaacaga actacgttca
      421 gcagattggt accactacca ttggtttcta cgttgaatac gaaaacatca acctgtctcg
      481 tcagactctg tactcttcca actttcgtaa cctgctgaac atctttggtg aagaagactt
      541 caaatacttc ctgatcgact tcctggtttt caccaaagtt gaacagaacg gttacctgca
      601 agttgcaggt gtttgtctga accagtactt ctctgttcag gtgaaacaga agaaatggta
      661 caagaacaac ttcaacatga acggtaaagc tactagtaac aataaccaga acaatgctaa
      721 cctgtccaac gagaagaaac aggagaacca gtacatctat ccagaaatcc aacgttctca
      781 aatcttctac tgtaaccaca tgggtcgtga a
//
