LOCUS       sequence-34459-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Min B, Collins K
  TITLE     An RPA-related sequence-specific DNA-binding subunit of telomerase 
            holoenzyme is required for elongation processivity and telomere 
            maintenance.
  JOURNAL   Mol Cell. 2009 Nov 25. 36(4):609-19.
  PUBMED    19941821
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 25. 36(4):609-19."
COMMENT     Sequence Label: pET28Teb1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcga aaacctgtac ttccagggcc atatgaagct gaccaaaggc
      121 ggttcctaca tcctgaaaaa ggtagaccgt aagcagttct accaggatga agaaatcgtt
      181 atgcagatca aaaagattct gggccagaaa actactgatt gcaaacagta tatcaaatgc
      241 gagtgcatcg atggcctggg tgacgaagca ctgatctact ttgagatgct ggcaaaccaa
      301 aaccagcacc tgcagaagaa cgacgttatc atgattcagg attacctgaa tgataaaact
      361 cagaacgata aaatcgtggt actggttacc cgcttccagt tttgcaaagc gtcccacgtt
      421 cagccgaaaa ccgcccagaa agagtccatc cagctgctga acaccgaaaa gaccatcatc
      481 cagaaatcta agatcactaa aaaccctgct gaagaagtgc tgaagttcat cgaggttaac
      541 gagaaagata actcttccaa ttctgaagat atgatcatcg agcagcagaa acaggagatt
      601 aaaaacaacc agaaagaaaa acagtctatc aatggcttca acctggagga ttcttacagc
      661 aacatttctg acatcactaa ctttggtggt aaaagcaatt tcaacatcgg tagcctgtcc
      721 gatcagctgt ctaaacagac tctgctgatt agccagctgc aggttggcaa aaatcgtttc
      781 agcttcaaat tcgagggccg cgtggtgtac aaatcctcta cttttcagaa tcagcaagat
      841 tccaaatact tcttcatcac cgcgca
//
