LOCUS       sequence-344595-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mma PylRS expression for ochre suppression in mammalian cells; 
            transient or piggy bac mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xMmaPylT(UUA)_EF1_MmaPylRS
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2727..2938
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2951..3219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3251..3260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3260..3283
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4667..5241,5242..5244,5245..5253)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4834..4851)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5061..5080
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5266..5865
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5266..5285)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5762..5782
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5925..5945)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5987..6108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6024..6043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6078..6097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6871..6905
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7396..7412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7396..7412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7409..7431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7420..7436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7444..7450,7451..7468,7469..7474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7489..7510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7627..7644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7798..8386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7878..7897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8557..9348,9349..9417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9180..9199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccaact agttctgcag tatttagcat
     1381 gccccaccca tctgcaaggc attctggata gtgtcaaaac agccggaaat caagtccgtt
     1441 tatctcaaac tttagcattt tgggaataaa tgatatttgc tatgctggtt aaattagatt
     1501 ttagttaaat ttcctgctga agctctagta cgataagtaa cttgacctaa gtgtaaagtt
     1561 gagatttcct tcaggtttat atagcttgtg cgccgcctgg gtacctcgga aacctgatca
     1621 tgtagatcga acggacttta aatccgttca gccgggttag attcccgggg tttccggaca
     1681 agtgcggttt ttcctgcagt atttagcatg ccccacccat ctgcaaggca ttctggatag
     1741 tgtcaaaaca gccggaaatc aagtccgttt atctcaaact ttagcatttt gggaataaat
     1801 gatatttgct atgctggtta aattagattt tagttaaatt tcctgctgaa gctctagtac
     1861 gataagtaac ttgacctaag tgtaaagttg agatttcctt caggtttata tagcttgtgc
     1921 gccgcctggg tacctcggaa acctgatcat gtagatcgaa cggactttaa atccgttcag
     1981 ccgggttaga ttcccggggt ttccggacaa gtgcggtttt tcctgcagta tttagcatgc
     2041 cccacccatc tgcaaggcat tctggatagt gtcaaaacag ccggaaatca agtccgttta
     2101 tctcaaactt tagcattttg ggaataaatg atatttgcta tgctggttaa attagatttt
     2161 agttaaattt cctgctgaag ctctagtacg ataagtaact tgacctaagt gtaaagttga
     2221 gatttccttc aggtttatat agcttgtgcg ccgcctgggt acctcggaaa cctgatcatg
     2281 tagatcgaac ggactttaaa tccgttcagc cgggttagat tcccggggtt tccggacaag
     2341 tgcggttttt cctgcagtat ttagcatgcc ccacccatct gcaaggcatt ctggatagtg
     2401 tcaaaacagc cggaaatcaa gtccgtttat ctcaaacttt agcattttgg gaataaatga
     2461 tatttgctat gctggttaaa ttagatttta gttaaatttc ctgctgaagc tctagtacga
     2521 taagtaactt gacctaagtg taaagttgag atttccttca ggtttatata gcttgtgcgc
     2581 cgcctgggta cctcggaaac ctgatcatgt agatcgaacg gactttaaat ccgttcagcc
     2641 gggttagatt cccggggttt ccggacaagt gcggtttttc actagtgctc tagaaaggat
     2701 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2761 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     2821 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2881 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     2941 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     3001 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     3061 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     3121 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     3181 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     3241 tctagagcta gccaccatgg actacaagga cgacgacgac aagatggaca agaagcccct
     3301 gaacaccctg atcagcgcca caggactgtg gatgtccaga accggcacca tccacaagat
     3361 caagcaccac gaggtgtccc ggtccaaaat ctacatcgag atggcctgcg gcgatcacct
     3421 ggtcgtcaac aacagcagaa gcagccggac agccagagcc ctgcggcacc acaagtacag
     3481 aaagacctgc aagcggtgca gagtgtccga cgaggacctg aacaagttcc tgaccaaggc
     3541 caacgaggac cagaccagcg tgaaagtgaa ggtggtgtcc gcccccaccc ggaccaagaa
     3601 agccatgccc aagagcgtgg ccagagcccc caagcccctg gaaaacaccg aagccgctca
     3661 ggcccagccc agcggcagca agttcagccc cgccatcccc gtgtctaccc aggaaagcgt
     3721 cagcgtcccc gccagcgtgt ccaccagcat ctctagcatc tcaaccggcg ccacagcttc
     3781 tgccctggtc aagggcaaca ccaaccccat caccagcatg tctgcccctg tgcaggcctc
     3841 tgccccagcc ctgaccaagt cccagaccga ccggctggaa gtgctcctga accccaagga
     3901 cgagatcagc ctgaacagcg gcaagccctt ccgggagctg gaaagcgagc tgctgagccg
     3961 gcggaagaag gacctccagc aaatctacgc cgaggaacgg gagaactacc tgggcaagct
     4021 ggaaagagag atcacccggt tcttcgtgga ccggggcttc ctggaaatca agagccccat
     4081 cctgatcccc ctggagtaca tcgagcggat gggcatcgac aacgacaccg agctgagcaa
     4141 gcagattttc cgggtggaca agaacttctg cctgcggccc atgctggccc ccaacctgta
     4201 caactacctg cggaaactgg atcgcgctct gcccgacccc atcaagattt tcgagatcgg
     4261 cccctgctac cggaaagaga gcgacggcaa agagcacctg gaagagttta caatgctgaa
     4321 cttttgccag atgggcagcg gctgcaccag agagaacctg gaatccatca tcaccgactt
     4381 tctgaaccac ctggggatcg acttcaagat cgtgggcgac agctgcatgg tgtacggcga
     4441 caccctggac gtgatgcacg gcgacctgga actgtctagc gccgtcgtgg gacccatccc
     4501 tctggaccgg gagtggggca tcgataagcc ctggatcgga gccggcttcg gcctggaacg
     4561 gctgctgaaa gtcaagcacg actttaagaa catcaagcgg gctgccagaa gcgagagcta
     4621 ctacaacggc atcagcacca acctgtgatg aggatccgcg gccgcgcccc tctccctccc
     4681 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4741 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4801 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4861 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4921 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4981 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5041 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5101 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5161 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5221 tttgaaaaac acgatgataa tatggccaca accatggcgt ccggaatgac cgagtacaag
     5281 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5341 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5401 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5461 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5521 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5581 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5641 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5701 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5761 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5821 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct
     5881 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5941 ctatgtggat acgctgcttt aatgcctttg tatcatgcgt taactaaact tgtttattgc
     6001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa
     6121 ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct tccgggggac
     6181 aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt
     6241 gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa
     6301 cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg cccattggta
     6361 tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc
     6421 agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc
     6481 tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc
     6541 ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata
     6601 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     6661 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     6721 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     6781 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     6841 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     6901 tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     6961 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     7021 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     7081 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     7141 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     7201 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     7261 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     7321 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     7381 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7441 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7501 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7561 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8521 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcat
//
