LOCUS       sequence-344597-        8927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of mouse short Adar2, blasticidine selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Hariharan A, Qi W, Rehrauer H, Wu L, Ronner M, Wipplinger M, 
            Kresoja-Rakic J, Sun S, Oton-Gonzalez L, Sculco M, Serre-Beinier V, 
            Meiller C, Blanquart C, Fonteneau JF, Vrugt B, Ruschoff JH, Opitz I,
            Jean D, de Perrot M, Felley-Bosco E
  TITLE     Heterogeneous RNA editing and influence of ADAR2 on mesothelioma 
            chemoresistance and the tumor microenvironment.
  JOURNAL   Mol Oncol. 2022 Dec;16(22):3949-3974. doi: 10.1002/1878-0261.13322. 
            Epub 2022 Oct 31.
  PUBMED    36221913
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1878-0261.13322"; journalName: "Mol Oncol"; date: 
            "2022-12"; volume: "16"; issue: "22"; pages: "3949-3974"
COMMENT     Sequence Label: BII-ChBtW-mADAR2-short
FEATURES             Location/Qualifiers
     source          1..8927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    454..588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(491..510)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     545..564
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    668..1256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             774..785
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1183..1203
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3396..3419)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3429..3482)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3501..3896)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      3893..3902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4835..4851
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4835..4851
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     repeat_region   5120..5154
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5645..5661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5645..5661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5658..5680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5669..5685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5693..5699,5700..5717,5718..5723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5738..5759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5876..5893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6047..6635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6127..6146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6806..7597,7598..7666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7429..7448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7667..7771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7797..8252)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7934..7955)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8146..8165
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8379..8401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8393..8410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8394..8410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8540..8602
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
       61 cctgggggct ttgggggggg gctgtccccg tgagctccat tggtatggct ttttccccgt
      121 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
      181 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
      241 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
      301 cgtaattaca tccctggggg ctttgggggg gggctgtccc tctagaggat cataatcagc
      361 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      421 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      481 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      541 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcac tagtatcgat
      601 atctcgactc actacctccc tttaaacgat cgacggccac gaagtgctta gccagaatta
      661 attccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
      721 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
      781 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
      841 agccatggaa aggacgtcag cttccccgac aacaccacgg aattgtcagt gcccaacagc
      901 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
      961 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     1021 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     1081 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     1141 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     1201 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattgttc
     1261 cagacgcgaa ctctagctca gggagtgaag gagaactggt cctgctctgt gggcttctcc
     1321 acccaggcgc ccagccccgc cttgatgaag gcagtgaaca gacgggcctt ggcagcctgg
     1381 tactccctcg ctgccagctt ggactcgtgg tatgtggtag gcttagtgat cttggtgcgc
     1441 agcaggtggg ggggaacctt gccgtgtaca cgcatccagc gacagtacag cgcgtgctta
     1501 cacaggcggg atgggcggcc aagctcatcc ttccctgttg tggcattgat gacctcaatg
     1561 gtggcgtcac ccactgtcca gttgacactg aagttgggtg ccttccctgg ctgccgtgcc
     1621 tctgcattgc tgatgccgct gagcaggggc ttgttgagag tgtagagcgg tggcaggtcc
     1681 tctatgttgg agatccgctg gtacatggcc ctggagaggt ggtccccgtg gtacaggctg
     1741 cccaagatga tgctggagaa gtagatgggt tccacgaaaa tgctgagcag ggacccctgg
     1801 atgcccacca cgttccagcg tgctatcttg tcactgcagg acatggtgag cagccgttcc
     1861 ccctgcagca ccccgtccca ggtctggatg ctggcatttg agcgcacagg gattgtcccc
     1921 tcgccagact ctattttcgt ccgtagctgt ccccttgctt tgcgattcgg atgtctatct
     1981 gctggttcct ctagcacggg ctcgtgggga gagaatattc tggcgtctcc gcagggcgag
     2041 gtgctgatgt acaggtggaa ctgcacggta tccttcagcc ggaacccacc ccgctctgac
     2101 ttctgaaata tggacttttt ctggtcttct ttgttattta aataaagctc gagctgtgca
     2161 taaagaaacc tgagcaggga ccttcgggag attatctctg cgtggcagtc attgagtgcg
     2221 aggccacggt cactcatgta ttcaccgttg atacacttcg tccctgtcga aacacttatc
     2281 accttggcat ctttgacatc tgtacctgtg gtcatcacta ctccagagag cactttcctt
     2341 cgtgcatgag gggaggaaaa gttgtctgtc aggtcactga acttacccag gaccaggcgg
     2401 gagacagcat ctgccaatac ctgtggcaaa tgcaactgaa gaccctcact gaggacaggc
     2461 tggcgagatg gtgtttggtc caagtgcaaa ttgaagacag tagccaaggc agactgtgca
     2521 gcccgggcct tggcaagctt cttgtttctt cctgagccct caaagaactg gccatctacc
     2581 accacggaca tgacgaagct cttggcgtgg ctctccccac tctcagagag gaagtcatac
     2641 ttcagccctg ggcgtagctc attcaagatc atcacgggat tcttcccact tgggggtggg
     2701 aatggtggtg ggatgggcag aggaggctgg gtaaggctgg caggcactgg ggaggccgat
     2761 aggctgacat ctccacttga gctgaacgag tcatccccgt tggagcctac gtagaagggt
     2821 ggctctgact tgtctggagt ctcgaagcca ttgaagagtg tgtcagggaa gtcagcctgg
     2881 tcagacgtga agtctgtgtt cacagagagg gtccttccca tggctaggtg ggcctcggag
     2941 gcgttgggaa actggacaaa agacctcagg gccttctcag cagcatggag ttttgccttt
     3001 ttctttgtag ggccagagcc ctcaaaaacc tgcccgttta cctccacaga catgacaaac
     3061 agaggtgcat gcacgggtcc tgtctgggac agcagcatgt actgtaagcc aggtttgatc
     3121 tcgttcagct gcatcagggc gttcttgggc agaacaggcc ctggtgtttt ccttcgcttc
     3181 ttcaggcggt acttggagtg accattgctg ccctcctcca ggggccgctt cctgctggtg
     3241 ctaccaccac ccccgttgga gagcggaata ccctcgccag gcccaggtgt gctgctgtcc
     3301 ttggggggca tgttgtccag attgcggttt tctttaatat cagtgctgct ggaactcata
     3361 ttctcttcat cttctatatc catgccgcta tcgagcttgt catcgtcgtc cttgtagtcc
     3421 atgccgagtg ggccgggatt ttcctccacg tccccgcatg ttagaagact tcccctgccc
     3481 tcgccggagc caaggctagc gccctcccac acataaccag agggcagcaa ttcacgaatc
     3541 ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga
     3601 tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg
     3661 atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg
     3721 agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg
     3781 tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt
     3841 gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg
     3901 gcaccggtcg tggcggccga cctgcaggtt cactaaaccg aatgttcaca gagactactg
     3961 cacttatata tggttctccc ccaccctggg gaaaaaggtg gagccagtac accacatcac
     4021 tttcccagtt tacccaagcc ccaccttctc taggcaccag ttcaattgcc cacccctccc
     4081 cccaacttct cagggactgt gggccatgtg ctctctgccc actgaggggc actcagccct
     4141 caagcatgct cttctccact agtcacccct attgacctta tgtatgtgcc aataatggga
     4201 aaaacccatt gactcacccc ctattgacct tttgtactgg gcaaaaccca atggaaagtc
     4261 cctattgact cagtgtactt ggctccaatg ggactttcct gttgattcac ccctattgac
     4321 cttatgtact gggcaaaacc cattggaaag tccctaatga ctcagtatac atgtcagtaa
     4381 tgggaaaaac ccattggctt acctcccatt gaccttatgt actgggcaaa acccattgga
     4441 aagtccctat tgactcaatg tacttggctc caatgggact ttcctgttga ctcaccccct
     4501 attgacctta tgtactgggc aaaacccaat ggaaagtccc tattgagtca gtgtacttgg
     4561 ctccaatggg tttttcccat tgactcctgc aggccttgga ggccttttcc ccgtatcccc
     4621 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     4681 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     4741 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     4801 tacatccctg ggggctttgg gggggggctg tccctctaga actagtggat ctataacaag
     4861 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     4921 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     4981 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5041 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     5101 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
     5161 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
     5221 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
     5281 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
     5341 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
     5401 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
     5461 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
     5521 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
     5581 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
     5641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     7801 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7861 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     7921 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     7981 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8041 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     8101 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     8161 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     8221 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     8281 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8341 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     8401 gacggccagt gagcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac
     8461 tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg acacgctgca
     8521 gaacacgcag ctagattaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
     8581 tcatgtgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
     8641 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
     8701 caatttattt atgtttattt atttattaaa aaaaacaaaa actcaaaatt tcttctataa
     8761 agtaacaaaa cttttatcga attctttttc cccgtatccc cccaggtgtc tgcaggctca
     8821 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
     8881 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggacc
//
