LOCUS       sequence-34460-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     Collins lab PP7CP plasmids
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET283xFlagPP7CPHis
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..106
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             500..517
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(570..588)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      584..631
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(755..774)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaataattt tgtttaactt taagaaggag atataccatg gactacaaag accatgacgg
       61 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagatgg ccaaaaccat
      121 cgttcttgcg gtcggcgagg ctactcgcac tctgactgag atccagtcca ccgcagaccg
      181 tcagatcttc gaagagaagg tcgggcctct ggtgggtcgg ctgcgcctca cggcttcgct
      241 ccgtcaaaac ggagccaaga ccgcgtatcg cgtcaaccta aaactggatc aggcggacgt
      301 cgttgatgca tccaccagcg tcgccggcga gcttccgaaa gtgcgctaca ctcaggtatg
      361 gtcgcacgac gtgacaatcg ttgcgaatag caccgaggcc tcgcgcaaat cgttgtacga
      421 tttgaccaag tccctcgtcg cgacctcgca ggtcgaagat cttgtcgtca accttgtgcc
      481 gctgggccgt tccctcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      541 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      601 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      661 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      841 cgatttagtg ctttacgg
//
