LOCUS       sequence-344601-        7613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DAXX protein for baculovirus expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Huang L, Agrawal T, Zhu G, Yu S, Tao L, Lin J, Marmorstein R, 
            Shorter J, Yang X
  TITLE     DAXX represents a new type of protein-folding enabler.
  JOURNAL   Nature. 2021 Aug 18. pii: 10.1038/s41586-021-03824-5. doi: 
            10.1038/s41586-021-03824-5.
  PUBMED    34408321
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Aug 18. pii: 10.1038/s41586-021-03824-5. doi: 
            10.1038/s41586-021-03824-5."
COMMENT     Sequence Label: pFastBac-GST-DAXX-His
FEATURES             Location/Qualifiers
     source          1..7613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2..655)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(22..44)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     promoter        complement(704..795)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(762..779)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(1147..1152,1153..1169,1170..1175)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1345..1878
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  1945..2169
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2303..2320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2474..3062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2554..2573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3233..4024,4025..4093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3856..3875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4094..4198)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4224..4678)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4361..4382)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4572..4591
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  4862..5027
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    5056..5190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5080..5099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5134..5153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5341..5358)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7588..7608)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attttggagg atggtcgcca ccaccaaacg tggcttgcca gccctgcaaa ggccatgcta
       61 tatacttgct ggatttcaag tacttatcaa tttgtgggat agcttcaata cgttttttaa
      121 aacaaactaa ttttgggaac gcatccaggc acattgggtc catgtataaa acaacatcaa
      181 gagcgtcata caacatgaag tcaggatggg ttacatgatc accatttaaa tatgttttat
      241 gacataaacg atcttcgaac attttcagca tttcaggtag cttgctaaga aaatcaactt
      301 tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac accgtatcta atatccaaaa
      361 ccgctccttc aagcattgaa atctctgcac gctcttttgg acaaccaccc aacatgttgt
      421 gcttgtcagc tatataacgt atgatggcca tagactgtgt taatttaaca tcaccatcaa
      481 tataataagg aagattggga aactccaaac ccaattcaaa ctttttgttt cgccatttat
      541 caccttcatc gcgctcatac aaatgctctt catatttttc ttcaagatat tccaaaagaa
      601 gtcgagtggg ttgcacaagg cccttaattt tccaataacc tagtataggg gacatggtgg
      661 tagatccgcg cccgatggtg ggacggtatg aataatccgg aatatttata ggttttttta
      721 ttacaaaact gttacgaaaa cagtaaaata cttatttatt tgcgagatgg ttatcatttt
      781 aattatctcc atgatctatt aatattccgg agtatacgta gccaaccact agaactatag
      841 ctagagtcct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
      901 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
      961 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
     1021 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
     1081 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     1141 acccagttga cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca
     1201 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1261 atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg
     1321 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1381 agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc
     1441 ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga
     1501 gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt
     1561 agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg
     1621 gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca
     1681 gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat
     1741 gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc
     1801 gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac
     1861 ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg
     1921 ttcggtaaat tgtcacaacg ccgcgaatat agtctttacc atgcccttgg ccacgcccct
     1981 ctttaatacg acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa
     2041 aagtccagta tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc
     2101 tagtttaaga ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt
     2161 ccgcccacac ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca
     2221 ggttacgcgg ctggtctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2281 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2881 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4201 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     4261 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4321 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4381 actattaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4441 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4501 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4561 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4621 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4681 cattcgccat tcaggctgca aataagcgtt gatattcagt caattacaaa cattaataac
     4741 gaagagatga cagaaaaatt ttcattctgt gacagagaaa aagtagccga agatgacggt
     4801 ttgtcacatg gagttggcag gatgtttgat taaaaacata acaggaagaa aaatgccccg
     4861 ctgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta
     4921 tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa
     4981 attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcgg atctcctagg
     5041 ctcaagcagt gatcagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     5101 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     5161 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     5221 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     5281 atggctgatt atgatcctct agtacttctc gacaagcttg gtaccgcatg cctcgagcta
     5341 atggtgatgg tgatgatgat cagagtctga aagcacgatg atctcttctg gatcgcattg
     5401 tgtggccaca cttgtcttgc aagtaccagg ccgaggaggc tgtgaatggg gggtttggga
     5461 cagccgggct ggagaaggga tgcagaggga gctggtcacc aggccatggc tgggagagtc
     5521 caccctcgtg gaggaatcag caactggggc caaggaagcc aagggggaag gtgggctggg
     5581 cagggtacat atctttttcc cattcttctc atgcactgac ctttgccttt ccacatagct
     5641 gtttcctaat ggccctgatc ctgtttgctt cttctccttc cgagattttt tgcagggggg
     5701 accagaatct ccccagttgt gaggagagac gcctccattg aaggaagtag aagagaccat
     5761 gcctgctcca ttctctaaga cagtggtgaa gggctcctct gattgcttcc tggaagagga
     5821 aatgtccgtc tccacagagg aaggggtatc caggggcaaa gcttcaatct ctagctcaaa
     5881 gagctgagac acagggcttt cttcctccag ggtcagctcc tcaggctgtt ctccattgct
     5941 ttcagcatct atgctggagg gggccagggg ttcttctgac agtaacgatg gtgacactat
     6001 gcgtcctttg ttttgctgct cccctgaaga tctgctgatc tgtttgccag gttccaggtt
     6061 cttttcattg gagatctgta gtgaggacat ggggctcttg tctccatctt tacctgctgc
     6121 tgcttcttcc tcttcgtcct cctcttcatc atcctcctga ccctcctgca tctgttccag
     6181 atcctcctcc tcttcagaat ctgtggcctc ctcctcttct tcttcctcct cctcctcctc
     6241 ttcctcatca ctctcctcat cgtcttcgtc atctgtctca gctctggagg cagaagggca
     6301 cccctgggat gccattccac tagggccctc accagaatcc aaggaggctt cgggggtgtc
     6361 tgcagagtgg gaagaggtgc cttggagccg agttcttctc ttttttctct cgccctcctc
     6421 acttttgtct tgcaacattg cgtatttgga gatgacctca tccagccgac tcatggccaa
     6481 actccggttt tcccgaaggc gccgggccaa cacaggatct gatagtgcag ggtcaacgcc
     6541 tggcctatag tcatctgtga ggtggcaacc aaagttgtag atgagatcga ggtgacgtcg
     6601 ctcctgtaac ctgatgccca catctcggaa gacatcctga gccatgagct ggagctgctg
     6661 tcgggggagg ccaaggctgt gtcgggcagc tgccttctct acagcccgaa gcacatcccc
     6721 atagtcaggg aaggtatcag gccctggctt gttgatgagc cgctcaatgc gcctgttaac
     6781 ctctgggtag cgggtgccac ggtaggggat gcgctgctct atgacacggc cggtcagtga
     6841 agagcagtct ttcagctcac atagtcgccc aaagaggcgg atcagcttac gcttcaaccg
     6901 tgcctcctgc aggtatgcgg agtctgggtc atccaattct gagagatcca actccttttc
     6961 ctgcagccgc cggatctctg ccacatagag cgccagcagc tgctccaaac gctggatctg
     7021 ccgccgggaa ccacgggtcc ttggagactg agaggcagtg ttttcagcat ttgtggggtc
     7081 caaggagagg tgtgtgggag ggttattccc agagggctca ttggaggtgg tggcggcagg
     7141 ggccaagttc agcttctttt tggctgagtg ggccttgaga acagtgcaga gctcattgat
     7201 gtagacatag agcttggctg gccggctccg ggcccgagac aggaccctag agaggatgtt
     7261 gcagaactcc gccgaggcca aaaacagaga gtgggcacgt tgctgccggt tatagaggaa
     7321 tgggaccacc tcagggtggt ctgctgtctg catcttacaa agttcaagga actcttcgaa
     7381 cagcttctca ttctccagct tgtagcattt cttgccgccc gaactactgc ttcctctggc
     7441 cccatgaggc tcagaggagc taggggcttc tgccccaggt gaggccgcat tggggagtgg
     7501 gtgggagggc cctggctgag cagctgcttc atcttcgtca tcatcatcca gcacgatgat
     7561 gctgttagcg gtggccatgg atccggcgcc ctgaaaatac aggttttcat ccg
//
