LOCUS       sequence-344603-        7237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DAXX protein for bacteria expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Huang L, Agrawal T, Zhu G, Yu S, Tao L, Lin J, Marmorstein R, 
            Shorter J, Yang X
  TITLE     DAXX represents a new type of protein-folding enabler.
  JOURNAL   Nature. 2021 Aug 18. pii: 10.1038/s41586-021-03824-5. doi: 
            10.1038/s41586-021-03824-5.
  PUBMED    34408321
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Aug 18. pii: 10.1038/s41586-021-03824-5. doi: 
            10.1038/s41586-021-03824-5."
COMMENT     Sequence Label: pGEX-DAXX-His
FEATURES             Location/Qualifiers
     source          1..7237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(235..240,241..258,259..265)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    273..289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     297..313
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     297..313
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             309..836
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(329..346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(329..345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(938..943,944..959,960..966)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    974..990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1013..1666
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1624..1646
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1673..1690
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1691..1711
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3938..3955
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4042..4064)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4209..4227)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4295..4399
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4400..4468,4469..5260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4618..4637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5431..6019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5920..5939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6173..6190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6263..6340
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(6341..7237,1..186)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6360..6379)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
       61 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
      121 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      181 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcacccc aggctttaca
      241 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      301 aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg
      361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      481 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
      541 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
      601 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
      661 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
      721 gccagacgcg aattattttt gatggcgttg gaattagctt atcgactgca cggtgcacca
      781 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
      841 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
      901 atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc atcggctcgt
      961 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagtat tcatgtcccc
     1021 tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata
     1081 tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa
     1141 caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt
     1201 taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg
     1261 tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag
     1321 atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct
     1381 tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt
     1441 aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt
     1501 atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat
     1561 tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt
     1621 gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg atctggttcc
     1681 gcgtggatct gagaacctgt acttccaggg atccgccacc gctaacagca tcatcgtgct
     1741 ggatgatgat gacgaagatg aagcagctgc tcagccaggg ccctcccacc cactccccaa
     1801 tgcggcctca cctggggcag aagcccctag ctcctctgag cctcatgggg ccagaggaag
     1861 cagtagttcg ggcggcaaga aatgctacaa gctggagaat gagaagctgt tcgaagagtt
     1921 ccttgaactt tgtaagatgc agacagcaga ccaccctgag gtggtcccat tcctctataa
     1981 ccggcagcaa cgtgcccact ctctgttttt ggcctcggcg gagttctgca acatcctctc
     2041 tagggtcctg tctcgggccc ggagccggcc agccaagctc tatgtctaca tcaatgagct
     2101 ctgcactgtt ctcaaggccc actcagccaa aaagaagctg aacttggccc ctgccgccac
     2161 cacctccaat gagccctctg ggaataaccc tcccacacac ctctccttgg accccacaaa
     2221 tgctgaaaac actgcctctc agtctccaag gacccgtggt tcccggcggc agatccagcg
     2281 tttggagcag ctgctggcgc tctatgtggc agagatccgg cggctgcagg aaaaggagtt
     2341 ggatctctca gaattggatg acccagactc cgcatacctg caggaggcac ggttgaagcg
     2401 taagctgatc cgcctctttg ggcgactatg tgagctgaaa gactgctctt cactgaccgg
     2461 ccgtgtcata gagcagcgca tcccctaccg tggcacccgc tacccagagg ttaacaggcg
     2521 cattgagcgg ctcatcaaca agccagggcc tgataccttc cctgactatg gggatgtgct
     2581 tcgggctgta gagaaggcag ctgcccgaca cagccttggc ctcccccgac agcagctcca
     2641 gctcatggct caggatgcct tccgagatgt gggcatcagg ttacaggagc gacgtcacct
     2701 cgatctcatc tacaactttg gctgccacct cacagatgac tataggccag gcgttgaccc
     2761 tgcactatca gatcctgtgt tggcccggcg ccttcgggaa aaccggagtt tggccatgag
     2821 tcggctggat gaggtcatct ccaaatacgc aatgttgcaa gacaaaagtg aggagggcga
     2881 gagaaaaaag agaagagctc ggctccaagg cacctcttcc cactctgcag acacccccga
     2941 agcctccttg gattctggtg agggccctag tggaatggca tcccaggggt gcccttctgc
     3001 ctccagagct gagacagatg acgaagacga tgaggagagt gatgaggaag aggaggagga
     3061 ggaggaagaa gaagaggagg aggccacaga ttctgaagag gaggaggatc tggaacagat
     3121 gcaggagggt caggaggatg atgaagagga ggacgaagag gaagaagcag cagcaggtaa
     3181 agatggagac aagagcccca tgtcctcact acagatctcc aatgaaaaga acctggaacc
     3241 tggcaaacag atcagcagat cttcagggga gcagcaaaac aaaggacgca tagtgtcacc
     3301 atcgttactg tcagaagaac ccctggcccc ctccagcata gatgctgaaa gcaatggaga
     3361 acagcctgag gagctgaccc tggaggaaga aagccctgtg tctcagctct ttgagctaga
     3421 gattgaagct ttgcccctgg ataccccttc ctctgtggag acggacattt cctcttccag
     3481 gaagcaatca gaggagccct tcaccactgt cttagagaat ggagcaggca tggtctcttc
     3541 tacttccttc aatggaggcg tctctcctca caactgggga gattctggtc ccccctgcaa
     3601 aaaatctcgg aaggagaaga agcaaacagg atcagggcca ttaggaaaca gctatgtgga
     3661 aaggcaaagg tcagtgcatg agaagaatgg gaaaaagata tgtaccctgc ccagcccacc
     3721 ttcccccttg gcttccttgg ccccagttgc tgattcctcc acgagggtgg actctcccag
     3781 ccatggcctg gtgaccagct ccctctgcat cccttctcca gcccggctgt cccaaacccc
     3841 ccattcacag cctcctcggc ctggtacttg caagacaagt gtggccacac aatgcgatcc
     3901 agaagagatc atcgtgctct cagactctga tgtcgaccac caccaccacc accactagct
     3961 cgacaagctt gatatcgcgg ccgcaattca tcgtgactga ctgacgatct gcctcgcgcg
     4021 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     4081 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     4141 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag
     4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4621 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4921 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6181 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6241 tatttcacac cgcataaatt ccgacaccat cgaatggcgc aaaacctttc gcggtatggc
     6301 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     6361 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     6421 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     6481 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     6541 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     6601 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     6661 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     6721 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     6781 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     6841 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     6901 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     6961 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     7021 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     7081 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     7141 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     7201 caccatcaaa caggattttc gcctgctggg gcaaacc
//
