LOCUS       sequence-344622-       10306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Small-molecule controlled base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, 
            Bartman CR, Lan Y, Green AM, Shi J, Kohli RM
  TITLE     Controllable genome editing with split-engineered base editors.
  JOURNAL   Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 
            10.1038/s41589-021-00880-w. Epub 2021 Oct 18.
  PUBMED    34663942
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00880-w"; journalName: "Nat Chem Biol"; date: 
            "2021-12"; volume: "17"; issue: "12"; pages: "1262-1270"
COMMENT     Sequence Label: CMV-evoA1-seBE4max-IRES
FEATURES             Location/Qualifiers
     source          1..10306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     215..234
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      411..420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(417..419,420..740)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             join(1335..1358,1359..1361,1362..5435)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1335..5435
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5745..5993
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6036..6056
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6065..6082
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6105..6122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6111..6335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6406..6422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6406..6422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6419..6441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6430..6446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6454..6460,6461..6478,6479..6484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6499..6520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6637..6654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6808..7396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6888..6907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7567..8358,8359..8427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8190..8209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8428..8532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8611..8630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8802..9181
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9182..9385
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9336..9356
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9427..9446
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9427..9445
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9453..9462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9462..9491
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9492..9539
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    join(10150..10306,1..396)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(join(10294..10306,1..5))
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
       61 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
      121 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
      181 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
      241 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
      301 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
      361 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc ccccaagctt gccaccatgg
      421 gcgtacaagt tgaaactatc agccctgggg acggcagaac ctttccgaag aggggacaga
      481 catgtgttgt tcactatact ggaatgttgg aagatggtaa gaagttcgat agcagcagag
      541 ataggaataa accatttaaa ttcatgcttg gcaagcaaga agtgattagg ggttgggaag
      601 aaggtgtcgc tcaaatgagt gtaggtcaga gggctaagtt aacaattagt cctgattatg
      661 cttatggcgc tacaggtcat ccaggaatca ttcccccaca tgctactctt gttttcgacg
      721 ttgaattgct taagcttgaa ggatcaggtt ctggatctgg ttcaggatca ggctcacccg
      781 ggcttaatac cagatgtagc atcacatggt ttctgagctg gtccccttgc ggagagtgta
      841 gcagggccat caccgagttc ctgtccagat atccaaatgt gacactgttt atctacatcg
      901 ccaggctgta tcacctggca aacccaagga ataggcaggg cctgcgcgat ctgatcagct
      961 ccggcgtgac catccagatc atgacagagc aggagtccgg ctactgctgg cacaacttcg
     1021 tgaattattc tcctagcaac gagtcccact ggcctaggta cccacacctg tgggtgcgcc
     1081 tgtacgtgct ggagctgtat tgcatcatcc tgggcctgcc cccttgtctg aatatcctgc
     1141 ggagaaagca gagccagctg acctccttta caatcgccct gcagtcttgt cactatcaga
     1201 ggctgccacc ccacatcctg tgggccacag gcctgaagtc tggcggatct agcggaggat
     1261 cctctggcag cgagacaccc gggacaagcg agtcagcaac accagagagc agtggcggca
     1321 gcagcggcgg cagcgacaag aagtacagca tcggcctggc catcggcacc aactctgtgg
     1381 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
     1441 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
     1501 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
     1561 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
     1621 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
     1681 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
     1741 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
     1801 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
     1861 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
     1921 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     1981 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     2041 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     2101 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     2161 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     2221 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     2281 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     2341 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     2401 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     2461 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     2521 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     2581 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     2641 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     2701 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     2761 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     2821 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     2881 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     2941 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     3001 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     3061 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     3121 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     3181 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     3241 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     3301 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     3361 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     3421 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     3481 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     3541 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     3601 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     3661 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     3721 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     3781 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     3841 atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     3901 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     3961 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     4021 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     4081 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     4141 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     4201 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     4261 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     4321 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     4381 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     4441 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     4501 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     4561 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     4621 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     4681 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     4741 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     4801 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     4861 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     4921 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     4981 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     5041 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     5101 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     5161 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     5221 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     5281 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     5341 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     5401 agacacggat cgacctgtct cagctgggag gtgacagcgg cgggagcggc gggagcgagg
     5461 ggagcactaa tctgagcgac atcattgaga agaagactgg gaaacagctg gtcattcagg
     5521 agtccatcct gatgctgcct gaggaggtgg aggaagtgat cggcaacaag ccagagtctg
     5581 acatcctggt gcacaccgcc tacgacgagt ccacagatga gaatgtgatg ctgctgacct
     5641 ctgacgcccc cgagtataag ccttgggccc tggtcatcca ggattctaac ggcgagaata
     5701 agatcaagat gctgagcgga ggatccggag gatctggagg cagcaccaac ctgtctgaca
     5761 tcatcgagaa ggagacaggc aagcagctgg tcatccagga gagcatcctg atgctgcccg
     5821 aagaagtcga agaagtgatc ggaaacaagc ctgagagcga tatcctggtc cataccgcct
     5881 acgacgagag taccgacgaa aatgtgatgc tgctgacatc cgacgcccca gagtataagc
     5941 cctgggctct ggtcatccag gattccaacg gagagaacaa aatcaaaatg ctgtctggcg
     6001 gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg aaagtctaac
     6061 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6361 gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6481 taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6541 cgctttccag tcaggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6601 aagaggcggt ttgcgtattg ggcgcacttc cgcttcctcg ctcactgact cgctgcgctc
     6661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7381 aaaaaaagga tctcaagaaa atcctttgat cttttctacg gggtctgaca ctcagtggaa
     7441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7681 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     7741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8521 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg
     8581 atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag
     8641 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     8701 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     8761 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     8821 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     8881 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     8941 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     9001 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     9061 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     9121 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     9181 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     9241 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     9301 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     9361 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagatccgc
     9421 ggccgctaat acgactcact atagggagag ccgccaccat ggaacaaaaa cttatttctg
     9481 aagaagatct gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     9541 gaggttccgc tagcggaggt tcgatgagtt caaagactgg gcctgtcgcc gtcgatccaa
     9601 ccctgcgccg ccggattgaa cctcacgagt ttgaagtgtt ctttgacccc cgggagctga
     9661 gaaaggagac atgcctgctg tacgagatca actggggagg caggcactcc atctggaggc
     9721 acacctctca gaacacaaat aagcacgtgg aggtgaactt catcgagaag tttaccacag
     9781 agcggtactt ctgccccggc gcgccgggag gtggtggcag cggtggagga ggttctgggg
     9841 gcggtggctc aattttatgg catgagatgt ggcatgaggg tttggaagag gcatctagat
     9901 tgtatttcgg tgaaagaaat gtcaagggaa tgttcgaagt tttagaaccg ttgcacgcta
     9961 tgatggagag aggtccacag actctaaagg agacttcctt caaccaagct tatggaaggg
    10021 acctaatgga ggctcaagaa tggtgtagaa aatacatgaa aagtggaaat gtaaaggacc
    10081 ttacacaagc ttgggatctc tactaccatg tttttaggag aatatctaaa taatgacccc
    10141 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
    10201 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
    10261 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaagg
//
