LOCUS       sequence-34463-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized
            for E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, Gartner 
            W, Petereit L, Efetova M, Schwarzel M, Oertner TG, Nagel G, Hegemann
            P
  TITLE     Light modulation of cellular cAMP by a small bacterial 
            photoactivated adenylyl cyclase, bPAC, of the soil bacterium 
            Beggiatoa.
  JOURNAL   J Biol Chem. 2011 Jan 14. 286(2):1181-8.
  PUBMED    21030594
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jan 14. 286(2):1181-8."
COMMENT     Sequence Label: pET28a-ec_bPAC
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             173..190
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cccgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
      181 gcgcggcagc catatgatga aacgcctggt gtatattagc aaaattagcg gccatctgag
      241 cctggaagaa attcagcgta ttggcaaagt gagcatcaaa aacaaccagc gcgataacat
      301 taccggcgtg ctgctgtatc tgcagggcct gttttttcag attctggaag gcgaaaacga
      361 aaaagtggat aaactgtata aaaaaatcct ggtggatgat cgtcatacca acattctgtg
      421 cctgaaaacc gaatatgata tcaccgatcg catgtttccg aactgggcca tgaaaaccat
      481 taacctgaac gaaaacagcg aactgatgat tcagccgatt aaaagcctgc tgcagaccat
      541 tacccagagc catcgtgtgc tggaaaaata tatgccggca cgtgtgattt atctgattaa
      601 ccagggcatt aatccgctga ccgtggaacc gcagctggtt gaaaaaatca tcttcttcag
      661 cgatattctg gcctttagca ccctgaccga aaaactgccg gtgaacgaag tggtgattct
      721 ggtgaaccgc tattttagca tttgcacccg tattattagc gcgtatggcg gcgaagtgac
      781 caaatttatt ggcgattgcg tgatggcgag ctttaccaaa gaacagggtg atgcggcgat
      841 tcgtaccagc ctggatatta tcagcgaact gaaacagctg cgtcatcatg tggaagcgac
      901 caatccgctg catctgctgt ataccggcat tggcctgagc tatggccatg tg
//
