LOCUS       sequence-344639-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in 
            FRET reflecting fluctuations in cAMP levels in living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ponsioen B, Zhao J, Riedl J, Zwartkruis F, van der Krogt G, Zaccolo 
            M, Moolenaar WH, Bos JL, Jalink K
  TITLE     Detecting cAMP-induced Epac activation by fluorescence resonance 
            energy transfer: Epac as a novel cAMP indicator.
  JOURNAL   EMBO Rep. 2004 Dec . 5(12):1176-80.
  PUBMED    15550931
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2004 Dec . 5(12):1176-80."
COMMENT     Sequence Label: Epac-S-H30
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(73..75,76..78,79..789)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3025..3027,3028..3030,3031..3741)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(3793..3811)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3794..3811)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3831..3848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3837..4061
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4107..4535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4194..4213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4404..4425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4544..4564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4549..4878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4729..4864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4791..4810
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4945..5739
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4999..5018)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5609..5628
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5913..6034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5950..5969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6004..6023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6083..6099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6083..6099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6096..6118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6107..6123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6131..6137,6138..6155,6156..6161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6176..6197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6314..6331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6485..7073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6565..6584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7244..8035,8036..8104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7867..7886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8105..8209)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8284..8303)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8475..8854
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8855..9058
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9009..9029
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tgccgccacc
       61 atggcggccg caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      541 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
      721 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      781 ctgtacaagt ccggactcag atctcgatat cagcccgtgg gaactcatga gatggaggag
      841 gagttggccg aagctgtggc cctgctctcc cagcgggggc ctgacgccct gctcactgtg
      901 gcacttcgaa agcccccagg tcagcgcacg gatgaagagc tggacctcat ctttgaggag
      961 ctgctgcaca tcaaggctgt ggcccacctc tccaactcgg tgaagcgaga attagcggct
     1021 gttctgctct ttgaaccaca cagcaaggca gggaccgtgt tgttcagcca gggggacaag
     1081 ggcacttcgt ggtacattat ctggaaggga tctgtcaacg tggtgaccca tggcaagggg
     1141 ctggtgacca ccctgcatga gggagatgat tttggacagc tggctctggt gaatgatgca
     1201 ccccgggcag ccaccatcat cctgcgagaa gacaactgtc atttcctgcg tgtggacaag
     1261 caggacttca accgtatcat caaggatgtg gaggcaaaga ccatgcggct ggaagaacat
     1321 ggcaaagtgg tgctggtgct ggagagagcc tctcagggcg ccggcccttc ccgaccccca
     1381 accccaggca ggaaccggta tacagtgatg tctggcaccc cagagaagat cctagagctt
     1441 ctgttggagg ccatgggacc agattccagt gctcatgacc caacagagac attcctcagc
     1501 gacttcctcc tgacccacag ggtcttcatg cccagcgccc aactctgcgc tgcccttctg
     1561 caccacttcc atgtggagcc tgcgggtggc agcgagcagg agcgcagcac ctacgtctgc
     1621 aacaagaggc agcagatctt gcggctggtc agccagtggg tggccctgta tggctccatg
     1681 ctccacactg accctgtggc caccagcttc ctccagaaac tctcagacct ggtgggcagg
     1741 gacacccgac tcagcaacct gctgagggag cagtggccag agaggcggcg atgccacagg
     1801 ttggagaatg gctgtgggaa tgcatctcct cagatgaagg cccggaactt gcctgtttgg
     1861 ctccccaacc aggacgagcc ccttcctggc agcagctgtg ccatccaagt tggggataaa
     1921 gtcccctatg acatctgccg gccagaccac tcagtgttga ccctgcagct gcctgtgaca
     1981 gcctccgtga gagaggtgat ggcagcgttg gcccaggagg atggctggac caaggggcag
     2041 gtgctggtga aggtcaattc tgcaggtgat gccattggcc tgcagccaga tgcccgtggt
     2101 gtggccacat ctctggggct caatgagcgt ctctttgttg tcaacccaca ggaagcgcat
     2161 gagctgatcc cacaccctga ccagctgggg cccactgtgg gctctgctga ggggctggac
     2221 ctggtgagtg ccaaggacct ggcaggccag ctgacggacc acgactggag cctcttcaac
     2281 agtatccacc aggtggagct gatccactat gtgctgggcc cccagcatct gcgggatgtc
     2341 accaccgcca acctggagcg cttcatgcgc cgcttcaatg agctgcagta ctgggtggcc
     2401 accgagctgt gtctctgccc cgtgcccggc ccccgggccc agctgctcag gaagttcatt
     2461 aagctggcgg cccacctcaa ggagcagaag aatctcaatt ccttctttgc cgtcatgttt
     2521 ggcctcagca actcggccat cagccgccta gcccacacct gggagcggct gccccacaaa
     2581 gtccggaagc tgtactccgc cctcgagagg ctgctggatc cctcatggaa ccaccgggta
     2641 taccgactgg ccctcgccaa gctctcccct cctgtcatcc ccttcatgcc ccttcttctc
     2701 aaagacatgg ccgccattca tgagggaaac cacacactag tggagaatct catcaacttt
     2761 gagaagatga gaatgatggc cagagccgcg cggatgctgc accactgccg aagccacaac
     2821 cctgtgcctc tctcaccact cagaagccga gtttcccacc tccacgagga cagccaggtg
     2881 gcgaggattt ccacatgctc ggagcagtcc ctgagcaccc ggagtccagc cagcacctgg
     2941 gcttatgtcc agcagctgaa ggtcattgac aaccagcggg aactctcccg cctctcccga
     3001 gagctggagc cagctagcga gctcatggtg agcaagggcg aggagctgtt caccggggtg
     3061 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3181 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcgt gcagtgcttc
     3241 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3481 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3601 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc
     3661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3721 ggcatggacg agctgtacaa gccgaattcc ctcgaggtta acgcggcgcg cctctaatct
     3781 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     3841 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3901 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3961 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4021 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4081 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4501 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4561 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4621 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4681 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4801 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4861 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     4921 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4981 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5041 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5101 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5161 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5221 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5281 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5341 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5401 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5461 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5521 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5581 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5641 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5701 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5761 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5821 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5881 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6061 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6901 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8221 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8281 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8341 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8401 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8461 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     8521 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     8581 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     8641 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     8701 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     8761 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     8821 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     8881 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     8941 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     9001 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     9061 ctggctaact agagaaccca ctg
//
