LOCUS       sequence-34464-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized
            for E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, Gartner 
            W, Petereit L, Efetova M, Schwarzel M, Oertner TG, Nagel G, Hegemann
            P
  TITLE     Light modulation of cellular cAMP by a small bacterial 
            photoactivated adenylyl cyclase, bPAC, of the soil bacterium 
            Beggiatoa.
  JOURNAL   J Biol Chem. 2011 Jan 14. 286(2):1181-8.
  PUBMED    21030594
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jan 14. 286(2):1181-8."
COMMENT     Sequence Label: pET28a-ec_bPAC
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 ttattattta tcgttttcca gggtctgacg aatcagctgg gtaatctgca gggtatcata
      121 atatttctgc gcttcgttca cggtataaac ttcaatcgct tcctgtttgc ctttcacctg
      181 atgcgcgccc aggttaataa aggtccactg cgcctggcag cattttttca cacccgcggt
      241 aaaggccagc gcatacggca gctgacgggt cagcgcttcc agacgcgccg caacgttcac
      301 cgcatcaccc agcagggtgt gatccatttt caggctgctg cccatgttgc cttcaatcac
      361 atggccatag ctcaggccaa tgccggtata cagcagatgc agcggattgg tcgcttccac
      421 atgatgacgc agctgtttca gttcgctgat aatatccagg ctggtacgaa tcgccgcatc
      481 accctgttct ttggtaaagc tcgccatcac gcaatcgcca ataaatttgg tcacttcgcc
      541 gccatacgcg ctaataatac gggtgcaaat gctaaaatag cggttcacca gaatcaccac
      601 ttcgttcacc ggcagttttt cggtcagggt gctaaaggcc agaatatcgc tgaagaagat
      661 gattttttca accagctgcg gttccacggt cagcggatta atgccctggt taatcagata
      721 aatcacacgt gccggcatat atttttccag cacacgatgg ctctgggtaa tggtctgcag
      781 caggctttta atcggctgaa tcatcagttc gctgttttcg ttcaggttaa tggttttcat
      841 ggcccagttc ggaaacatgc gatcggtgat atcatattcg gttttcaggn aa
//
