LOCUS       sequence-344640-        8993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in
            FRET reflecting fluctuations in cAMP levels in living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   van der Krogt GN, Ogink J, Ponsioen B, Jalink K
  TITLE     A comparison of donor-acceptor pairs for genetically encoded FRET 
            sensors: application to the Epac cAMP sensor as an example.
  JOURNAL   PLoS ONE. 2008  . 3(4):e1916.
  PUBMED    18382687
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(4):e1916."
COMMENT     Sequence Label: Epac-S-H81
FEATURES             Location/Qualifiers
     source          1..8993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(38..40,41..43,44..556)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(83..104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(344..363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     691..712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2942..3616
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             2942..3616
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3072..3090)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3340..3359
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3534..3557
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(3668..3686)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3669..3686)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3706..3723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3712..3936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3982..4410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4069..4088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4279..4300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4419..4439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4424..4753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4604..4739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4666..4685
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4820..5614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4874..4893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5484..5503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5788..5909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5825..5844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5879..5898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5958..5974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5958..5974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5971..5993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5982..5998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6006..6012,6013..6030,6031..6036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6051..6072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6189..6206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6360..6948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6440..6459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7119..7910,7911..7979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7742..7761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7980..8084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8159..8178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8350..8729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8730..8933
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8884..8904
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     join(8978..8993,1..4)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(8978..8993,1..3)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agggagaccc aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct
       61 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      121 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      181 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      241 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      301 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      361 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      421 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      481 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      541 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      601 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      661 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      721 ccgatatcag cccgtgggaa ctcatgagat ggaggaggag ttggccgaag ctgtggccct
      781 gctctcccag cgggggcctg acgccctgct cactgtggca cttcgaaagc ccccaggtca
      841 gcgcacggat gaagagctgg acctcatctt tgaggagctg ctgcacatca aggctgtggc
      901 ccacctctcc aactcggtga agcgagaatt agcggctgtt ctgctctttg aaccacacag
      961 caaggcaggg accgtgttgt tcagccaggg ggacaagggc acttcgtggt acattatctg
     1021 gaagggatct gtcaacgtgg tgacccatgg caaggggctg gtgaccaccc tgcatgaggg
     1081 agatgatttt ggacagctgg ctctggtgaa tgatgcaccc cgggcagcca ccatcatcct
     1141 gcgagaagac aactgtcatt tcctgcgtgt ggacaagcag gacttcaacc gtatcatcaa
     1201 ggatgtggag gcaaagacca tgcggctgga agaacatggc aaagtggtgc tggtgctgga
     1261 gagagcctct cagggcgccg gcccttcccg acccccaacc ccaggcagga accggtatac
     1321 agtgatgtct ggcaccccag agaagatcct agagcttctg ttggaggcca tgggaccaga
     1381 ttccagtgct catgacccaa cagagacatt cctcagcgac ttcctcctga cccacagggt
     1441 cttcatgccc agcgcccaac tctgcgctgc ccttctgcac cacttccatg tggagcctgc
     1501 gggtggcagc gagcaggagc gcagcaccta cgtctgcaac aagaggcagc agatcttgcg
     1561 gctggtcagc cagtgggtgg ccctgtatgg ctccatgctc cacactgacc ctgtggccac
     1621 cagcttcctc cagaaactct cagacctggt gggcagggac acccgactca gcaacctgct
     1681 gagggagcag tggccagaga ggcggcgatg ccacaggttg gagaatggct gtgggaatgc
     1741 atctcctcag atgaaggccc ggaacttgcc tgtttggctc cccaaccagg acgagcccct
     1801 tcctggcagc agctgtgcca tccaagttgg ggataaagtc ccctatgaca tctgccggcc
     1861 agaccactca gtgttgaccc tgcagctgcc tgtgacagcc tccgtgagag aggtgatggc
     1921 agcgttggcc caggaggatg gctggaccaa ggggcaggtg ctggtgaagg tcaattctgc
     1981 aggtgatgcc attggcctgc agccagatgc ccgtggtgtg gccacatctc tggggctcaa
     2041 tgagcgtctc tttgttgtca acccacagga agcgcatgag ctgatcccac accctgacca
     2101 gctggggccc actgtgggct ctgctgaggg gctggacctg gtgagtgcca aggacctggc
     2161 aggccagctg acggaccacg actggagcct cttcaacagt atccaccagg tggagctgat
     2221 ccactatgtg ctgggccccc agcatctgcg ggatgtcacc accgccaacc tggagcgctt
     2281 catgcgccgc ttcaatgagc tgcagtactg ggtggccacc gagctgtgtc tctgccccgt
     2341 gcccggcccc cgggcccagc tgctcaggaa gttcattaag ctggcggccc acctcaagga
     2401 gcagaagaat ctcaattcct tctttgccgt catgtttggc ctcagcaact cggccatcag
     2461 ccgcctagcc cacacctggg agcggctgcc ccacaaagtc cggaagctgt actccgccct
     2521 cgagaggctg ctggatccct catggaacca ccgggtatac cgactggccc tcgccaagct
     2581 ctcccctcct gtcatcccct tcatgcccct tcttctcaaa gacatggccg ccattcatga
     2641 gggaaaccac acactagtgg agaatctcat caactttgag aagatgagaa tgatggccag
     2701 agccgcgcgg atgctgcacc actgccgaag ccacaaccct gtgcctctct caccactcag
     2761 aagccgagtt tcccacctcc acgaggacag ccaggtggcg aggatttcca catgctcgga
     2821 gcagtccctg agcacccgga gtccagccag cacctgggct tatgtccagc agctgaaggt
     2881 cattgacaac cagcgggaac tctcccgcct ctcccgagag ctggagccag ctagcgagct
     2941 catggcctcc tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
     3001 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3061 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     3121 cctgtcccct cagttccagt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3181 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3241 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3301 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3361 catgggctgg gaggcctcca ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3421 gatcaagatg aggctgaagc tgaaggacgg cggccactac gacgccgagg tcaagaccac
     3481 ctacatggcc aagaagcccg tgcagctgcc cggcgcctac aagaccgaca tcaagctgga
     3541 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg
     3601 ccactccacc ggcgccccga attccctcga ggttaacgcg gcgcgcctct aatctagagg
     3661 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     3721 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3781 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3841 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3901 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3961 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4021 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4081 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4141 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4201 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4261 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4321 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4381 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4501 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4561 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4621 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4681 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4741 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4801 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4861 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4921 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4981 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5041 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5101 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5161 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5221 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5281 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5341 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5401 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5461 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5521 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5581 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5641 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5701 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5761 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5881 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8101 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     8161 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     8221 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     8281 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     8341 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     8401 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     8461 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     8521 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     8581 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     8641 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     8701 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     8761 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     8821 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     8881 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     8941 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tat
//
