LOCUS       sequence-344641-        9875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure 
            realtime changes in FRET reflecting fluctuations in cAMP levels in 
            living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   van der Krogt GN, Ogink J, Ponsioen B, Jalink K
  TITLE     A comparison of donor-acceptor pairs for genetically encoded FRET 
            sensors: application to the Epac cAMP sensor as an example.
  JOURNAL   PLoS ONE. 2008  . 3(4):e1916.
  PUBMED    18382687
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(4):e1916."
COMMENT     Sequence Label: Epac-S-H96
FEATURES             Location/Qualifiers
     source          1..9875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(114..116,117..119,120..830)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3066..3800
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             join(3858..3860,3861..3863,3864..4574)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(4626..4644)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4627..4644)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4664..4681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4670..4894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4940..5368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5027..5046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5237..5258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5377..5397)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5382..5711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5562..5697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5624..5643
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5778..6572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5832..5851)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6442..6461
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6746..6867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6783..6802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6837..6856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6916..6932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6916..6932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6929..6951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6940..6956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6964..6970,6971..6988,6989..6994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7009..7030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7147..7164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7318..7906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7398..7417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8077..8868,8869..8937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8700..8719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8938..9042)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9117..9136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9308..9687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9688..9875,1..16)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9842..9862
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgccgccac catggcggcc gcaatggtga
      121 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      181 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      241 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      301 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      361 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      421 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      481 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      541 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
      601 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      661 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      721 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      781 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
      841 gatctcgata tcagcccgtg ggaactcatg agatggagga ggagttggcc gaagctgtgg
      901 ccctgctctc ccagcggggg cctgacgccc tgctcactgt ggcacttcga aagcccccag
      961 gtcagcgcac ggatgaagag ctggacctca tctttgagga gctgctgcac atcaaggctg
     1021 tggcccacct ctccaactcg gtgaagcgag aattagcggc tgttctgctc tttgaaccac
     1081 acagcaaggc agggaccgtg ttgttcagcc agggggacaa gggcacttcg tggtacatta
     1141 tctggaaggg atctgtcaac gtggtgaccc atggcaaggg gctggtgacc accctgcatg
     1201 agggagatga ttttggacag ctggctctgg tgaatgatgc accccgggca gccaccatca
     1261 tcctgcgaga agacaactgt catttcctgc gtgtggacaa gcaggacttc aaccgtatca
     1321 tcaaggatgt ggaggcaaag accatgcggc tggaagaaca tggcaaagtg gtgctggtgc
     1381 tggagagagc ctctcagggc gccggccctt cccgaccccc aaccccaggc aggaaccggt
     1441 atacagtgat gtctggcacc ccagagaaga tcctagagct tctgttggag gccatgggac
     1501 cagattccag tgctcatgac ccaacagaga cattcctcag cgacttcctc ctgacccaca
     1561 gggtcttcat gcccagcgcc caactctgcg ctgcccttct gcaccacttc catgtggagc
     1621 ctgcgggtgg cagcgagcag gagcgcagca cctacgtctg caacaagagg cagcagatct
     1681 tgcggctggt cagccagtgg gtggccctgt atggctccat gctccacact gaccctgtgg
     1741 ccaccagctt cctccagaaa ctctcagacc tggtgggcag ggacacccga ctcagcaacc
     1801 tgctgaggga gcagtggcca gagaggcggc gatgccacag gttggagaat ggctgtggga
     1861 atgcatctcc tcagatgaag gcccggaact tgcctgtttg gctccccaac caggacgagc
     1921 cccttcctgg cagcagctgt gccatccaag ttggggataa agtcccctat gacatctgcc
     1981 ggccagacca ctcagtgttg accctgcagc tgcctgtgac agcctccgtg agagaggtga
     2041 tggcagcgtt ggcccaggag gatggctgga ccaaggggca ggtgctggtg aaggtcaatt
     2101 ctgcaggtga tgccattggc ctgcagccag atgcccgtgg tgtggccaca tctctggggc
     2161 tcaatgagcg tctctttgtt gtcaacccac aggaagcgca tgagctgatc ccacaccctg
     2221 accagctggg gcccactgtg ggctctgctg aggggctgga cctggtgagt gccaaggacc
     2281 tggcaggcca gctgacggac cacgactgga gcctcttcaa cagtatccac caggtggagc
     2341 tgatccacta tgtgctgggc ccccagcatc tgcgggatgt caccaccgcc aacctggagc
     2401 gcttcatgcg ccgcttcaat gagctgcagt actgggtggc caccgagctg tgtctctgcc
     2461 ccgtgcccgg cccccgggcc cagctgctca ggaagttcat taagctggcg gcccacctca
     2521 aggagcagaa gaatctcaat tccttctttg ccgtcatgtt tggcctcagc aactcggcca
     2581 tcagccgcct agcccacacc tgggagcggc tgccccacaa agtccggaag ctgtactccg
     2641 ccctcgagag gctgctggat ccctcatgga accaccgggt ataccgactg gccctcgcca
     2701 agctctcccc tcctgtcatc cccttcatgc cccttcttct caaagacatg gccgccattc
     2761 atgagggaaa ccacacacta gtggagaatc tcatcaactt tgagaagatg agaatgatgg
     2821 ccagagccgc gcggatgctg caccactgcc gaagccacaa ccctgtgcct ctctcaccac
     2881 tcagaagccg agtttcccac ctccacgagg acagccaggt ggcgaggatt tccacatgct
     2941 cggagcagtc cctgagcacc cggagtccag ccagcacctg ggcttatgtc cagcagctga
     3001 aggtcattga caaccagcgg gaactctccc gcctctcccg agagctggag ccagctagcg
     3061 agctcatgga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3121 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     3181 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3241 ctctcggcat ggacgagctg tacaagggtg gcagcggtgg catggtgagc aagggcgagg
     3301 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3361 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     3421 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     3481 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3541 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3601 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3661 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3721 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3781 agatccgcca caacatcgag ccgaattttg tcttcctgat cggcgccgca ggaatactct
     3841 tcgtatctag cgagctcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3901 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3961 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     4021 ccgtgccctg gcccaccctc gtgaccactc tgggctacgg cctacagtgc ttcgcccgct
     4081 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4141 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4201 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4261 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
     4321 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     4381 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4441 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     4501 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4561 acgagctgta caagccgaat tccctcgagg ttaacgcggc gcgcctctaa tctagagggc
     4621 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     4681 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4741 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4801 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4861 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4921 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4981 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5041 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5101 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5161 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5221 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5281 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5341 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5401 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5461 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5521 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5581 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5641 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5701 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     5761 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5821 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5881 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5941 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6001 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6061 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6121 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6181 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6241 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6301 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6361 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6421 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6481 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6541 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6601 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6661 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6721 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6841 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6901 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6961 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7021 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7081 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7261 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7321 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7381 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7441 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7501 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7561 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7621 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7681 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7741 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7801 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8041 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8101 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8161 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8221 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8281 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8341 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8821 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8881 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9061 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     9121 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     9181 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     9241 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     9301 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     9361 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9421 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9481 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9541 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     9601 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9661 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     9721 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     9781 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     9841 acgcaaatgg gcggtaggcg tgtacggtgg gaggt
//
