LOCUS       sequence-344646-        9083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime
            changes in FRET reflecting fluctuations in cAMP levels in living 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Klarenbeek J, Goedhart J, van Batenburg A, Groenewald D, Jalink K
  TITLE     Fourth-generation epac-based FRET sensors for cAMP feature 
            exceptional brightness, photostability and dynamic range: 
            characterization of dedicated sensors for FLIM, for ratiometry and 
            with high affinity.
  JOURNAL   PLoS One. 2015 Apr 14;10(4):e0122513. doi: 
            10.1371/journal.pone.0122513. eCollection 2015.
  PUBMED    25875503
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-14"; volume: "10(4):e0122513. doi"; pages: " 
            10.1371/journal.pone.0122513. eCollection 2015"
COMMENT     Sequence Label: Epac-S-H150
FEATURES             Location/Qualifiers
     source          1..9083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     84..103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        84..102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      119..128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(167..169,170..172,173..685)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             3071..3805
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYIMADKQKNGIKVNFKIRHNIE"
     promoter        complement(3857..3875)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3858..3875)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3895..3912)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3901..4125
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4171..4599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4258..4277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4468..4489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4608..4628)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4613..4942
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4793..4928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4855..4874
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5009..5803
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5063..5082)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5673..5692
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5977..6098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6014..6033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6068..6087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6147..6163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6147..6163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6160..6182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6171..6187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6195..6201,6202..6219,6220..6225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6240..6261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6378..6395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6549..7137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6629..6648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7308..8099,8100..8168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7931..7950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8169..8273)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8348..8367)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8539..8918
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8919..9083,1..39)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     join(9073..9083,1..10)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
       61 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttgccgc
      121 caccatgggg ggttctcatc atcatcatca tcatggtgcg gccgcaatgg tgagcaaggg
      181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      301 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      361 gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      601 ctactttagc gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      661 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
      721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      781 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      841 gaccgccgcc cgatatcagc ccgtgggaac tcatgagatg gaggaggagt tggccgaagc
      901 tgtggccctg ctctcccagc gggggcctga cgccctgctc actgtggcac ttcgaaagcc
      961 cccaggtcag cgcacggatg aagagctgga cctcatcttt gaggagctgc tgcacatcaa
     1021 ggctgtggcc cacctctcca actcggtgaa gcgagaatta gcggctgttc tgctctttga
     1081 accacacagc aaggcaggga ccgtgttgtt cagccagggg gacaagggca cttcgtggta
     1141 cattatctgg aagggatctg tcaacgtggt gacccatggc aaggggctgg tgaccaccct
     1201 gcatgaggga gatgattttg gacagctggc tctggtgaat gatgcacccc gggcagccac
     1261 catcatcctg cgagaagaca actgtcattt cctgcgtgtg gacaagcagg acttcaaccg
     1321 tatcatcaag gatgtggagg caaagaccat gcggctggaa gaacatggca aagtggtgct
     1381 ggtgctggag agagcctctc agggcgccgg cccttcccga cccccaaccc caggcaggaa
     1441 ccggtataca gtgatgtctg gcaccccaga gaagatccta gagcttctgt tggaggccat
     1501 gggaccagat tccagtgctc atgacccaac agagacattc ctcagcgact tcctcctgac
     1561 ccacagggtc ttcatgccca gcgcccaact ctgcgctgcc cttctgcacc acttccatgt
     1621 ggagcctgcg ggtggcagcg agcaggagcg cagcacctac gtctgcaaca agaggcagca
     1681 gatcttgcgg ctggtcagcc agtgggtggc cctgtatggc tccatgctcc acactgaccc
     1741 tgtggccacc agcttcctcc agaaactctc agacctggtg ggcagggaca cccgactcag
     1801 caacctgctg agggagcagt ggccagagag gcggcgatgc cacaggttgg agaatggctg
     1861 tgggaatgca tctcctcaga tgaaggcccg gaacttgcct gtttggctcc ccaaccagga
     1921 cgagcccctt cctggcagca gctgtgccat ccaagttggg gataaagtcc cctatgacat
     1981 ctgccggcca gaccactcag tgttgaccct gcagctgcct gtgacagcct ccgtgagaga
     2041 ggtgatggca gcgttggccc aggaggatgg ctggaccaag gggcaggtgc tggtgaaggt
     2101 caattctgca ggtgatgcca ttggcctgca gccagatgcc cgtggtgtgg ccacatctct
     2161 ggggctcaat gagcgtctct ttgttgtcaa cccacaggaa gcgcatgagc tgatcccaca
     2221 ccctgaccag ctggggccca ctgtgggctc tgctgagggg ctggacctgg tgagtgccaa
     2281 ggacctggca ggccagctga cggaccacga ctggagcctc ttcaacagta tccaccaggt
     2341 ggagctgatc cactatgtgc tgggccccca gcatctgcgg gatgtcacca ccgccaacct
     2401 ggagcgcttc atgcgccgct tcaatgagct gcagtactgg gtggccaccg agctgtgtct
     2461 ctgccccgtg cccggccccc gggcccagct gctcaggaag ttcattaagc tggcggccca
     2521 cctcaaggag cagaagaatc tcaattcctt ctttgccgtc atgtttggcc tcagcaactc
     2581 ggccatcagc cgcctagccc acacctggga gcggctgccc cacaaagtcc ggaagctgta
     2641 ctccgccctc gagaggctgc tggatccctc atggaaccac cgggtatacc gactggccct
     2701 cgccaagctc tcccctcctg tcatcccctt catgcccctt cttctcaaag acatggccgc
     2761 cattcatgag ggaaaccaca cactagtgga gaatctcatc aactttgaga agatgagaat
     2821 gatggccaga gccgcgcgga tgctgcacca ctgccgaagc cacaaccctg tgcctctctc
     2881 accactcaga agccgagttt cccacctcca cgaggacagc caggtggcga ggatttccac
     2941 atgctcggag cagtccctga gcacccggag tccagccagc acctgggctt atgtccagca
     3001 gctgaaggtc attgacaacc agcgggaact ctcccgcctc tcccgagagc tggagccagc
     3061 tagcagctca atgggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3121 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     3181 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3241 cactctcggc atggacgagc tgtacaaggg cggtaccggc ggcagcatgg tgagcaaggg
     3301 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3361 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3421 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     3481 cggctacggc ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     3541 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3601 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3661 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3721 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3781 cttcaagatc cgccacaaca tcgagccgaa ttccctcgag gttaacgcgg cgcgcctcta
     3841 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     3901 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3961 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4021 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4081 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4141 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4201 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4261 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4321 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4381 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4441 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4501 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4561 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4621 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4681 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4741 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4801 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4861 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4921 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4981 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5041 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5101 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5161 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5221 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5281 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5341 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5401 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5461 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5521 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5581 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5641 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5701 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5761 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5821 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5881 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5941 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6001 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6061 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6121 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6181 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6241 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6301 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6361 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8281 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     8341 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8401 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8461 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     8521 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     8581 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8641 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8701 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8761 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8821 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8881 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8941 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     9001 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     9061 ccgccccatt gacgcaaatg ggc
//
