LOCUS       sequence-344649-       15418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Harant A, Pai H, Sakai T, Kamoun S, Adachi H
  TITLE     A vector system for fast-forward studies of the HOPZ-ACTIVATED 
            RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana 
            benthamiana.
  JOURNAL   Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 
            10.1093/plphys/kiab471.
  PUBMED    34633454
REFERENCE   2  (bases 1 to 15418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2022-01-20- 20"; volume: "188"; issue: "1"; 
            pages: "70-80"
COMMENT     Sequence Label: pZA69
FEATURES             Location/Qualifiers
     source          1..15418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        409..753
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     697..717
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          1356..2952
                     /gene="pdk"
                     /number=3
                     /label=PDK intron
                     /note="modified pyruvate orthophosphate dikinase intron 
                     from Flaveria trinervia"
     CDS             1694..2377
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVVQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     terminator      3438..3690
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3699..3716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3699..3715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3708..3730)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3855..3874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4065..4086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    4333..4357
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5953..7101)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7400..8194)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGGADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  8386..9153
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      9776..10487
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(10614..10985)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11018..11127
                     /note="incP origin of transfer"
     primer_bind     11350..11369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11603..11620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(11808..12458)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    12923..12947
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        13071..13250
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        13103..13286
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             13307..14101
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13361..13380)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13971..13990
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      14491..14743
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    15292..15313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15328..15333,15334..15351,15352..15358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15366..15382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15371..15393
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15390..15406
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15390..15406
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctttcaa catgtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
       61 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      121 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      181 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga
      241 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
      301 aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg acacacttgt
      361 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
      421 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
      481 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacgtcgaga gttctcaaca caacatatac
      781 aaaacaaacg aatctcaagc aatcaagcat tctacttcta ttgcagcaat ttaaatcatt
      841 tcttttaaag caaaagcaat tttctgaaaa ttttcaccat ttacgaacga tagccagggc
      901 ccggaggtgg atgcggtggt cactgtattc ttggagaaac ttctgcatgt tctcacagag
      961 gagagtaggt ttcttactaa atacagacag cagtttgaaa aactgaagaa cgagctgcta
     1021 ttcatgcaaa gctttctcaa agatgcagag aggctcaaga ggaaaaacaa cacccttaaa
     1081 ggagtcatgt cctgtttgcg agacttaatc tttgaagctg aagagatact tgaggactgc
     1141 cagaatcaat ctgcagatag cgatcgggct actacatgct tccatcctaa aaggctctct
     1201 cttcgccatc aaactgggaa gtgccttgct aaaatcaatg acagaatctc ggagataaag
     1261 caaaacattt caacatatct tggagtacca cttctcgaag aaggaagtat ggaggcacac
     1321 aataatctaa tgtcaagatg gactacgagc ccttggtaag gaaataatta ttttcttttt
     1381 tccttttagt ataaaatagt taagtgatgt taattagtat gattataata atatagttgt
     1441 tataattgtg aaaaaataat ttataaatat attgtttaca taaacaacat agtaatgtaa
     1501 aaaaatatga caagtgatgt gtaagacgaa gaagataaaa gttgagagta agtatattat
     1561 ttttaatgaa tttgatcgaa catgtaagat gatatacggc cgaagaggtt ccaactttca
     1621 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     1681 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1741 gcatcgtaaa gaacattttg aggcatttca gtcagttgtt caatgtacct ataaccagac
     1801 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     1861 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     1921 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1981 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     2041 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     2101 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     2161 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     2221 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     2281 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     2341 gggggggcgt aaacgccgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     2401 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtcggccca taatagtaat
     2461 tctagctggt ttgatgaatt aaatatcaat gataaaatac tatagtaaaa ataagaataa
     2521 ataaattaaa ataatatttt tttatgatta atagtttatt atataattaa atatctatac
     2581 cattactaaa tattttagtt taaaagttaa taaatatttt gttagaaatt ccaatctgct
     2641 tgtaatttat caataaacaa aatattaaat aacaagctaa agtaacaaat aatatcaaac
     2701 taatagaaac agtaatctaa tgtaacaaaa cataatctaa tgctaatata acaaagcgca
     2761 agatctatca attttatata gtattatttt caatcaacat tctcattaat ttctaaataa
     2821 tacttgtagt tttattaact tctaaatgga ttgactatta attaaatgaa ttagtcgaac
     2881 atgaataaac aaggtaacat gatagatcat gtcattgtgt tatcattgat cttacatttg
     2941 gattgattac agttggtcta gagatttcgt ctagatcgta gtccatcttg acattagatt
     3001 attgtgtgcc tccatacttc cttcttcgag aagtggtact ccaagatatg ttgaaatgtt
     3061 ttgctttatc tccgagattc tgtcattgat tttagcaagg cacttcccag tttgatggcg
     3121 aagagagagc cttttaggat ggaagcatgt agtagcccga tcgctatctg cagattgatt
     3181 ctggcagtcc tcaagtatct cttcagcttc aaagattaag tctcgcaaac aggacatgac
     3241 tcctttaagg gtgttgtttt tcctcttgag cctctctgca tctttgagaa agctttgcat
     3301 gaatagcagc tcgttcttca gtttttcaaa ctgctgtctg tatttagtaa gaaacctact
     3361 ctcctctgtg agaacatgca gaagtttctc caagaataca gtgaccaccg catccacctc
     3421 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3481 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     3541 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     3601 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     3661 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
     3721 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3781 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3841 ctgaatggcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3961 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4081 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     4141 gatttcggaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     4201 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     4261 gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     4321 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     4381 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     4441 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     4501 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     4561 ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc
     4621 gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa
     4681 ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat
     4741 ttctttagaa gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac
     4801 aggtcgggga cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag
     4861 atctggggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     4921 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     4981 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     5041 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
     5101 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
     5161 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
     5221 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
     5281 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
     5341 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
     5401 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
     5461 ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca
     5521 cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga
     5581 ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc
     5641 gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct
     5701 ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc
     5761 caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca
     5821 gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt
     5881 gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga
     5941 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
     6001 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
     6061 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
     6121 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
     6181 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
     6241 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
     6301 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
     6361 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
     6421 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
     6481 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
     6541 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
     6601 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
     6661 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
     6721 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
     6781 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
     6841 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc
     6901 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
     6961 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
     7021 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
     7081 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
     7141 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
     7201 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
     7261 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc
     7321 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc
     7381 gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat tttattttct
     7441 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
     7501 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccccgccgc
     7561 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
     7621 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
     7681 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
     7741 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
     7801 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
     7861 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
     7921 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
     7981 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
     8041 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
     8101 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
     8161 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
     8221 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
     8281 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
     8341 gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac
     8401 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     8461 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
     8521 tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc
     8581 ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg
     8641 ggcaacagcc cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc
     8701 aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa
     8761 atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg
     8821 tatgacagtc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
     8881 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
     8941 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
     9001 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
     9061 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
     9121 ataaaacact atcaataagt tggagtcatt acccaattat gatagaattt acaagctata
     9181 aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct
     9241 atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg
     9301 atatcttcta tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc
     9361 tgcctcctca tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata
     9421 gagtaattct gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac
     9481 ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact
     9541 atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg
     9601 acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg
     9661 tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg
     9721 ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc
     9781 atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc
     9841 gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca
     9901 cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg
     9961 ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt
    10021 tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg
    10081 cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca
    10141 ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa
    10201 tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc
    10261 ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc
    10321 ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg
    10381 gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc
    10441 cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga aaggcgctcg gtcttgcctt
    10501 gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg gagcgcatgg
    10561 ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc
    10621 tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg
    10681 atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg
    10741 gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc
    10801 tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc
    10861 aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg
    10921 tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca
    10981 gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga
    11041 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
    11101 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
    11161 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
    11221 atcgctataa tgaccccgaa gcagggttat gcagcggaaa agcgccacgc ttcccgaagg
    11281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    11341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    11461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    11521 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    11581 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgccagaa
    11641 ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa
    11701 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
    11761 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
    11821 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
    11881 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
    11941 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
    12001 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
    12061 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
    12121 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
    12181 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
    12241 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
    12301 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
    12361 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
    12421 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
    12481 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
    12541 ggaggctggt ccggaggcca gacgtgaaac ccaacatacc cctgatcgta attctgagca
    12601 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
    12661 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
    12721 cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
    12781 ggccaatctt gctcgtctcg ctggccggcg ccagatctgg ggaaccctgt ggttggcatg
    12841 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    12901 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    12961 taaactgaag gcgggaaacg acaatctgat catgagcgga gaattaaggg agtcacgtta
    13021 tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac
    13081 gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca catacgtcag
    13141 aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt
    13201 gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata
    13261 ttcactctca atccaaataa tctgcaccgg atctggatcg tttcgcatga ttgaacaaga
    13321 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
    13381 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
    13441 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
    13501 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    13561 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    13621 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    13681 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    13741 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    13801 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt
    13861 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    13921 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    13981 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    14041 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    14101 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
    14161 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    14221 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca cgggatctct
    14281 gcggaacagg cggtcgaagg tgccgatatc attacgacag caacggccga caagcacaac
    14341 gccacgatcc tgagcgacaa tatgatcggg cccggcgtcc acatcaacgg cgtcggcggc
    14401 gactgcccag gcaagaccga gatgcaccgc gatatcttgc tgcgttcgga tattttcgtg
    14461 gagttcccgc cacagacccg gatgatcccc gatcgttcaa acatttggca ataaagtttc
    14521 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
    14581 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
    14641 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
    14701 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggcctc ctgtcaatgc
    14761 tggcggcggc tctggtggtg gttctggtgg cggctctgag ggtggtggct ctgagggtgg
    14821 cggttctgag ggtggcggct ctgagggagg cggttccggt ggtggctctg gttccggtga
    14881 ttttgattat gaaaagatgg caaacgctaa taagggggct atgaccgaaa atgccgatga
    14941 aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg attacggtgc
    15001 tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg gtgctactgg
    15061 tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata attcaccttt
    15121 aatgaataat ttccgtcaat atttaccttc cctccctcaa tcggttgaat gtcgcccttt
    15181 tgtctttggc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    15241 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    15301 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    15361 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgcc
//
