LOCUS       sequence-344659-        4787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone for Golden Gate assembly .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Harant A, Pai H, Sakai T, Kamoun S, Adachi H
  TITLE     A vector system for fast-forward studies of the HOPZ-ACTIVATED 
            RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana 
            benthamiana.
  JOURNAL   Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 
            10.1093/plphys/kiab471.
  PUBMED    34633454
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2022-01-20- 20"; volume: "188"; issue: "1"; 
            pages: "70-80"
COMMENT     Sequence Label: pZA54
FEATURES             Location/Qualifiers
     source          1..4787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3133..3144
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaatggtt gatgctgttg ttacagtttt tcttgaaaag cttcttcatg
     2281 ttcttactga agaatctaga tttttgacta agtatagaca acagttcgag aagcttaaga
     2341 acgaacttct tttcatgcag tctttcctta aggacgctga aagacttaaa agaaagaata
     2401 acactttgaa gggtgtgatg tcttgcctta gggacttgat cttcgaggct gaggagatct
     2461 tggaggattg ccagaaccag tctgctgact ctgatagagc tactacttgc ttccacccta
     2521 agaggttgtc tcttaggcac cagactggaa agtgtcttgc aaagattaat gataggattt
     2581 ctgaaattaa gcagaatatt tctacatatc ttggtgtgcc tcttttggag gagggttcta
     2641 tggaggctca caataacctt atgtctagat ggacatcttc cctttatgac cacactcagg
     2701 tagttggttt ggaaggtgac actgagaaga taaaggattg gctatttgaa gcaagagatg
     2761 gtttacttac cattgcattt gtgggtatgg gaggacttgg taaaaccact cttgctcaga
     2821 aagttttcaa tgacaaaagg gtggaggatc acttggagag gaggatttgg gtgtctgttt
     2881 ctcaaacatt tactgaggaa caagtcatga gaagcatatt gaggagttta ggagatgcat
     2941 gtgttggtga tgaccagtgt gaactgttaa gaaaaataaa ccagtacctt ttgggaaaga
     3001 ggtttttgat tgttatggat gatgtttgga gctgggacaa tgcctggtgg caaaaaatct
     3061 atactggact gcccaaagga aatgggagca ctgttattgt aacaacgaga aatgagctag
     3121 tcgctcgcaa gatgggagtt acagaagcaa gaatacattg gccaaaattc ctcaatgagc
     3181 actacagttg gttactattc cggaagattg catttgcagg aagtgcaggt gaatgccatt
     3241 tccccgagtt ggaggacgtg ggaaaggaga ttgtggaaaa atgcaaggga cttcctttag
     3301 caatcaaagc agtaggagga gtgatgcttt gcaagccatc ttactatcac gaatggaggc
     3361 gtatttcaaa tcatttccgt gatgaattga aagaaaatga tgactcagtg atggcctcac
     3421 tacagttgag ctatgatgaa ctccctcctt acttgaaatc ctgtttcctc tgtttttcac
     3481 tctttcctga ggattgtgtc ataccaaaag accaactgat ccgttggtgg attggagaag
     3541 gtttcatacc tctgagaagt ggtaggttat cgactgaagt gggagaagat tgtttctcac
     3601 aactatccaa tcggtgtttg atagaagttg ttgataaggc ttacaatggt gtgatccaca
     3661 catgcaaaat gcatgttatg gttcgtgact tggtgatcaa attagccgag gatgatgcat
     3721 ttttcacccc agctgatgca acttgtcggc atttgggtat taagagtgag atgaattgga
     3781 agcaactatt gagcaatcaa aaattacgag cactgctaac aaccaccaag agtggtgaag
     3841 ttaacaaaat ccattccgac attgccaaga agctgtgcaa gagtcgacac ctccaagtat
     3901 tggatctgtc aaaatcaatt ttcgatgtgc ctctttcaag tttgttggaa ggaattggat
     3961 ctgctaagca gctcacttat ctcagtttaa gcaatacaca tccaatgatt ggagttccag
     4021 cttccatatc caagcttgaa aaattacaga ttttggattt cagctattgc caaaatatga
     4081 aaatgctccc ctcttgcgtt ctgacatttg aggaacttgc tgttttggat gtgaacaact
     4141 gtggttccct tgagtacctt ccaaaaggat taagtaggct ttccaatctt caagtattgc
     4201 ttgggtttaa gcctgcaaaa ttaagccaac caggaggttg tcgtattgct gagctcagaa
     4261 gcctgactcg actcagaact ctcagtttaa gactaaccga aaatgaggag attggagacg
     4321 atgaggggaa cgcactggta gatctccaag aacttcaatt tttaacaata agttgctttg
     4381 gcagtcaaga taatggactt gcgacaaaac ttggtagact ttatcctcca cgacaactcc
     4441 acgagctgat tctcaaattt tatccaagta agacaagtcc tgaatggctt aaccccaatc
     4501 tctcccccat gttgcgatat ttatcaatca tctctggtga tattacacaa atgcacgaga
     4561 acttctgggg tgatggtagt actgcttgga aaattgaggg cttaatgttg gaatctttat
     4621 cggatttgag attggaatgg tcagcaatgc atcaagtcat gccttcgtta agaatactca
     4681 aggttagctg gtgccctgag ttggagtcat ttccaattga agatgcaggg tttagagggg
     4741 gtctatggaa gaaggaagaa cataggaacg gttcgtgaga ccacgaa
//
