LOCUS       sequence-344661-       10215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Small-molecule controlled base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, 
            Bartman CR, Lan Y, Green AM, Shi J, Kohli RM
  TITLE     Controllable genome editing with split-engineered base editors.
  JOURNAL   Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 
            10.1038/s41589-021-00880-w. Epub 2021 Oct 18.
  PUBMED    34663942
REFERENCE   2  (bases 1 to 10215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00880-w"; journalName: "Nat Chem Biol"; date: 
            "2021-12"; volume: "17"; issue: "12"; pages: "1262-1270"
COMMENT     Sequence Label: CMV-A3A-seBE4max-IRES
FEATURES             Location/Qualifiers
     source          1..10215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      652..661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             661..690
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             691..738
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    1372..1924
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1516..1533)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1743..1762
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1939..1948
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1945..1947,1948..2268)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             join(2749..2772,2773..2775,2776..6849)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2749..6849
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7159..7407
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7450..7470
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7479..7496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7519..7536)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7525..7749
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7820..7836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7820..7836)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7833..7855)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7844..7860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7868..7874,7875..7892,7893..7898))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7913..7934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8051..8068)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8222..8810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8302..8321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8981..9772,9773..9841))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9604..9623
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9842..9946)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10025..10044)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gaacaaaaac ttatttctga agaagatctg aaaaggccgg cggccacgaa aaaggccggc
      721 caggcaaaaa agaaaaaggg aggttccgct agcggaggtt cgatggaagc cagcccagca
      781 tccgggccca gacacttgat agatccacac atattcactt ccaactttaa caatggcatt
      841 ggaaggcata agacctacct gtgctacgaa gtggagcgcc tggacaatgg cacctcggtc
      901 aagatggacc agcacagggg ctttctacac aaccaggcta agaatcttct ctgtggcttt
      961 tacggccgcc atgcggagct gcgcttcttg gacctggttc cttctttgca gttggacccg
     1021 ggcgcgccgg gaggtggtgg cagcggtgga ggaggttctg ggggcggtgg ctcaatttta
     1081 tggcatgaga tgtggcatga gggtttggaa gaggcatcta gattgtattt cggtgaaaga
     1141 aatgtcaagg gaatgttcga agttttagaa ccgttgcacg ctatgatgga gagaggtcca
     1201 cagactctaa aggagacttc cttcaaccaa gcttatggaa gggacctaat ggaggctcaa
     1261 gaatggtgta gaaaatacat gaaaagtgga aatgtaaagg accttacaca agcttgggat
     1321 ctctactacc atgtttttag gagaatatct aaataatgac ccccccccct aacgttactg
     1381 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     1441 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     1501 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     1561 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     1621 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     1681 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     1741 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     1801 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     1861 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     1921 aatatggccc ccaagcttgc caccatgggc gtacaagttg aaactatcag ccctggggac
     1981 ggcagaacct ttccgaagag gggacagaca tgtgttgttc actatactgg aatgttggaa
     2041 gatggtaaga agttcgatag cagcagagat aggaataaac catttaaatt catgcttggc
     2101 aagcaagaag tgattagggg ttgggaagaa ggtgtcgctc aaatgagtgt aggtcagagg
     2161 gctaagttaa caattagtcc tgattatgct tatggcgcta caggtcatcc aggaatcatt
     2221 cccccacatg ctactcttgt tttcgacgtt gaattgctta agcttgaagg atcaggttct
     2281 ggatctggtt caggatcagg ctcacccggg cttgcccaga tctacagggt cacctggttc
     2341 atctcctgga gcccctgctt ctcctggggc tgtgccgggg aagtgcgtgc gttcctgcag
     2401 gagaacacac acgtgagact gcgtatcttc gctgcccgca tctatgatta cgacccccta
     2461 tataaggagg cactgcaaat gctgcgggat gctggggccc aagtctccat catgacctac
     2521 gatgaattta agcactgctg ggacaccttt gtggaccacc agggatgtcc cttccagccc
     2581 tgggatggac tagatgagca cagccaagcc ctgagtggga ggctgcgggc cattctccag
     2641 aatcagggaa actctggcgg atctagcgga ggatcctctg gcagcgagac acccgggaca
     2701 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcga caagaagtac
     2761 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     2821 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     2881 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     2941 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     3001 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     3061 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     3121 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     3181 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     3241 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     3301 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     3361 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     3421 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     3481 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     3541 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     3601 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     3661 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     3721 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     3781 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     3841 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     3901 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     3961 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     4021 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     4081 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     4141 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     4201 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     4261 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     4321 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     4381 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     4441 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     4501 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     4561 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     4621 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     4681 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     4741 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     4801 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     4861 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     4921 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     4981 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     5041 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     5101 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     5161 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     5221 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     5281 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     5341 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     5401 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     5461 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     5521 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     5581 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     5641 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     5701 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     5761 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     5821 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     5881 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     5941 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     6001 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     6061 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     6121 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     6181 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     6241 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     6301 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     6361 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     6421 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     6481 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     6541 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     6601 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     6661 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     6721 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     6781 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     6841 ggaggtgaca gcggcgggag cggcgggagc gaggggagca ctaatctgag cgacatcatt
     6901 gagaagaaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag
     6961 gtggaggaag tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac
     7021 gagtccacag atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg
     7081 gccctggtca tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc
     7141 ggaggatctg gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag
     7201 ctggtcatcc aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac
     7261 aagcctgaga gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg
     7321 atgctgctga catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc
     7381 aacggagaga acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc cgacggcagc
     7441 gaattcgagc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag
     7501 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7561 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7621 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7681 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7741 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
     7801 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7861 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
     7921 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcagga aacctgtcgt
     7981 gccagctgca ttaatgaatc ggccaacgcg cgggaagagg cggtttgcgt attgggcgca
     8041 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8101 cagctcactc aaaggcagta atacggttat ccacagaatc aggggataac gcaggaaaga
     8161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8401 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8641 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaaaatcctt
     8821 tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa gggattttgg
     8881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9121 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9961 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
    10021 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    10081 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    10141 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    10201 ccagatatac gcgtt
//
