LOCUS       sequence-344662-       12604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base Editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, 
            Bartman CR, Lan Y, Green AM, Shi J, Kohli RM
  TITLE     Controllable genome editing with split-engineered base editors.
  JOURNAL   Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 
            10.1038/s41589-021-00880-w. Epub 2021 Oct 18.
  PUBMED    34663942
REFERENCE   2  (bases 1 to 12604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00880-w"; journalName: "Nat Chem Biol"; date: 
            "2021-12"; volume: "17"; issue: "12"; pages: "1262-1270"
COMMENT     Sequence Label: Lenti-evoA1-BE4max
FEATURES             Location/Qualifiers
     source          1..12604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1492..1609
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1678..1889
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1909..1918
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1918..1983
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2020..2040
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2821..2844,2845..2847,2848..6921)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2821..6921
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6952..7200
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7231..7479
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7522..7542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7558..7614
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7615..8214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7615..7634)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8111..8131
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8230..8818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8283..8303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8701..8712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8890..9123
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9201..9322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9238..9257)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9292..9311
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9362..9497
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9517..9536)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9518..9536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9546..9563)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9546..9562)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9555..9577)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9704..10159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9791..9810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10001..10022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10185..10289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10290..10358,10359..11150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10508..10527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11321..11909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11810..11829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12063..12080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12197..12218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12233..12238,12239..12256,12257..12263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12271..12287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12276..12298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12295..12311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12295..12311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12330..12350
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12332..12350
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12378..12604
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagaccaca attttaaaag
     1501 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     1561 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     1621 cagggacagc agagatccac tttggcgccg gctcgagtgg ctccggtgcc cgtcagtggg
     1681 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     1741 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     1801 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     1861 ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg cgggatccgc caccatggac
     1921 tataaggacc acgacggaga ctacaaggat catgatattg attacaaaga cgatgacgat
     1981 aagatgaaac ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc
     2041 agttcaaaga ctgggcctgt cgccgtcgat ccaaccctgc gccgccggat tgaacctcac
     2101 gagtttgaag tgttctttga cccccgggag ctgagaaagg agacatgcct gctgtacgag
     2161 atcaactggg gaggcaggca ctccatctgg aggcacacct ctcagaacac aaataagcac
     2221 gtggaggtga acttcatcga gaagtttacc acagagcggt acttctgccc caataccaga
     2281 tgtagcatca catggtttct gagctggtcc ccttgcggag agtgtagcag ggccatcacc
     2341 gagttcctgt ccagatatcc aaatgtgaca ctgtttatct acatcgccag gctgtatcac
     2401 ctggcaaacc caaggaatag gcagggcctg cgcgatctga tcagctccgg cgtgaccatc
     2461 cagatcatga cagagcagga gtccggctac tgctggcaca acttcgtgaa ttattctcct
     2521 agcaacgagt cccactggcc taggtaccca cacctgtggg tgcgcctgta cgtgctggag
     2581 ctgtattgca tcatcctggg cctgccccct tgtctgaata tcctgcggag aaagcagagc
     2641 cagctgacct cctttacaat cgccctgcag tcttgtcact atcagaggct gccaccccac
     2701 atcctgtggg ccacaggcct gaagtctggc ggatctagcg gaggatcctc tggcagcgag
     2761 acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc
     2821 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     2881 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     2941 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     3001 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     3061 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     3121 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     3181 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     3241 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     3301 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     3361 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     3421 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     3481 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     3541 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     3601 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     3661 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     3721 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     3781 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     3841 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     3901 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     3961 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     4021 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     4081 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     4141 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     4201 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     4261 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     4321 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     4381 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     4441 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     4501 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     4561 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     4621 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     4681 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     4741 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     4801 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     4861 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     4921 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     4981 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     5041 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     5101 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     5161 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     5221 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     5281 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     5341 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     5401 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     5461 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     5521 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     5581 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     5641 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     5701 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     5761 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     5821 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     5881 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     5941 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     6001 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     6061 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     6121 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     6181 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     6241 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     6301 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     6361 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     6421 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     6481 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     6541 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     6601 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     6661 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     6721 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     6781 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     6841 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     6901 ctgtctcagc tgggaggtga cagcggcggg agcggcggga gcggggggag cactaatctg
     6961 agcgacatca ttgagaagga gactgggaaa cagctggtca ttcaggagtc catcctgatg
     7021 ctgcctgagg aggtggagga agtgatcggc aacaagccag agtctgacat cctggtgcac
     7081 accgcctacg acgagtccac agatgagaat gtgatgctgc tgacctctga cgcccccgag
     7141 tataagcctt gggccctggt catccaggat tctaacggcg agaataagat caagatgctg
     7201 agcggaggat ccggaggatc tggaggcagc accaacctgt ctgacatcat cgagaaggag
     7261 acaggcaagc agctggtcat ccaggagagc atcctgatgc tgcccgaaga agtcgaagaa
     7321 gtgatcggaa acaagcctga gagcgatatc ctggtccata ccgcctacga cgagagtacc
     7381 gacgaaaatg tgatgctgct gacatccgac gccccagagt ataagccctg ggctctggtc
     7441 atccaggatt ccaacggaga gaacaaaatc aaaatgctgt ctggcggctc aaaaagaacc
     7501 gccgacggca gcgaattcga gcccaagaag aagaggaaag tcgctagcgg cagcggcgcc
     7561 accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg ccccatgacc
     7621 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7681 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7741 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7801 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7861 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7921 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7981 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8041 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     8101 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8161 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt
     8221 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     8641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     8821 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8881 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8941 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9001 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9061 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9121 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9181 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     9241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9301 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9361 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9421 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9481 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     9541 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9601 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9661 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     9721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9901 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10021 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    10141 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    10201 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10261 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    10321 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    10381 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    10441 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    10501 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    10561 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    10621 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10681 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    10741 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10801 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10861 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    10921 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    10981 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    11041 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    11101 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    11161 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    11221 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    11281 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    11341 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    11401 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    11461 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    11521 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    11581 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11641 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11701 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11761 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11821 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11881 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11941 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    12001 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    12061 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    12121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    12181 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    12241 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    12301 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    12361 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
    12421 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
    12481 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
    12541 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
    12601 aaac
//
