LOCUS       sequence-34467-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Tsai WW, Wang Z, Yiu TT, Akdemir KC, Xia W, Winter S, Tsai CY, Shi 
            X, Schwarzer D, Plunkett W, Aronow B, Gozani O, Fischle W, Hung MC, 
            Patel DJ, Barton MC
  TITLE     TRIM24 links a non-canonical histone signature to breast cancer.
  JOURNAL   Nature. 2010 Dec 16. 468(7326):927-32.
  PUBMED    21164480
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Dec 16. 468(7326):927-32."
COMMENT     Sequence Label: Flag-TRIM24-C840W
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gcagactaca aggacgacga tgacaaggca
      121 gactacaagg acgacgatga caagggtacc tctagaagat ctaggacaat ggaggtggcg
      181 gtggagaagg cggtggcggc ggcggcagcg gcctcggctg cggcctccgg ggggccctcg
      241 gcggcgccga gcggggagaa cgaggccgag agtcggcagg gcccggactc ggagcgcggc
      301 ggcgaggcgg cccggctcaa cctgttggac acttgcgccg tgtgccacca gaacatccag
      361 agccgggcgc ccaagctgct gccctgcctg cactctttct gccagcgctg cctgcccgcg
      421 ccccagcgct acctcatgct gcccgcgccc atgctgggct cggccgagac cccgccaccc
      481 gtccctgccc ccggctcgcc ggtcagcggc tcgtcgccgt tcgccaccca agttggagtc
      541 attcgttgcc cagtttgcag ccaagaatgt gccgagagac acatcataga taactttttt
      601 gtgaaggaca ctactgaggt tcccagcagt acagtagaaa agtcaaatca ggtatgtaca
      661 agctgtgagg acaacgcaga agccaatggg ttttgtgtag agtgtgttga atggctctgc
      721 aagacgtgta tcag
//
