LOCUS       sequence-344671-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse Myc with T58A point mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Mollaoglu G, Guthrie MR, Bohm S, Bragelmann J, Can I, Ballieu PM, 
            Marx A, George J, Heinen C, Chalishazar MD, Cheng H, Ireland AS, 
            Denning KE, Mukhopadhyay A, Vahrenkamp JM, Berrett KC, Mosbruger TL,
            Wang J, Kohan JL, Salama ME, Witt BL, Peifer M, Thomas RK, Gertz J, 
            Johnson JE, Gazdar AF, Wechsler-Reya RJ, Sos ML, Oliver TG
  TITLE     MYC Drives Progression of Small Cell Lung Cancer to a Variant 
            Neuroendocrine Subtype with Vulnerability to Aurora Kinase 
            Inhibition.
  JOURNAL   Cancer Cell. 2017 Feb 13;31(2):270-285. doi: 
            10.1016/j.ccell.2016.12.005. Epub 2017 Jan 12.
  PUBMED    28089889
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.12.005"; journalName: "Cancer Cell"; date: 
            "2017-02-13- 13"; volume: "31"; issue: "2"; pages: "270-285"
COMMENT     Sequence Label: PIG-MycT58A
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..664
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(65..84)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     561..581
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 aaggcttgcg gcgctgttct ctcttctcat ctccgggcct ttcgacctgc agcccaagct
       61 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
      121 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
      181 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
      241 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
      301 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
      361 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
      421 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
      481 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
      541 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
      601 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
      661 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
      721 caattccgcc cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
      781 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
      841 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaagga
      901 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttt cttgaagaca
      961 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctgacga caggtgcctc
     1021 tgcgtcaaag cacgtgtata agatacactg ccaaggcgtc actatcccca gtgcacgtgt
     1081 gacttgcata gctggtgcca g
//
