LOCUS       sequence-344688-        7015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty vector for cloning shRNA and/or miRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7015)
  TITLE     Improved vector system for knockdown
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-H1-SX
FEATURES             Location/Qualifiers
     source          1..7015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..76
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(295..399)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      471..606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    647..781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(684..703)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     738..757
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             949..1582
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1629..1754
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2251..2484
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2669..2713
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3011..3128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3177..3267
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     3188..3207
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     enhancer        3366..3669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3670..3873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3824..3844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3870..3894
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4233..4821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4286..4306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4704..4715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5028..5261
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5362..5379)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5368..5479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(5690..6278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5770..5789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6449..7015,1..225,226..294))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
       61 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      421 acctgacgtc gagttagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      541 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
      601 gaggcctagg cttttgcaaa aagcttcgtc gacggatcgg gagatcaact tgtttattgc
      661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
      781 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
      841 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
      901 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
      961 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     1021 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     1081 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     1141 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     1201 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     1261 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     1321 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     1381 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1441 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1501 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1561 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     1621 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     1681 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     1741 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1801 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1861 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1921 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1981 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     2041 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     2101 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     2161 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     2221 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     2281 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     2341 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     2401 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     2461 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     2521 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     2581 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     2641 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     2701 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     2761 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2821 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2881 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2941 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     3001 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3061 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3121 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta ctagtaatat
     3181 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg
     3241 aatcttataa gttctgtatg agaccacatc gattcttttt ttctagacgt attaccgcca
     3301 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     3361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     3421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     3481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     3541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     3601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     3661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     3721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     3781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     3841 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     3901 agacgccatc cagcgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     3961 cggccgggaa cggtgcattg gaacgcggat tccccgtgta caagtagcgg ccgaatgagg
     4021 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
     4081 ttcaggttaa cccaacagaa ttctcttctg aagaaaatat atttcttttt attcatagct
     4141 cttctcgagg taaccggatc caaccagttg tgttttaatg tatattaatg ttactaatgt
     4201 gttttattta aattgagtca cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     4261 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4321 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4381 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4441 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4501 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4561 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4621 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4681 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4741 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4801 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     4861 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4921 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4981 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     5041 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5101 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5161 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5221 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5281 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     5341 ttgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5401 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5461 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5521 tggggcagga cagcaagggg gaggattggg aagacaatag cagggtatca gctcactcaa
     5581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6121 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtc
//
