LOCUS       sequence-344692-        6171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pOPIN-GG expression vector compatible with N-terminal tagging, 
            Kanamycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6171)
  TITLE     Banfield lab plasmids
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPGN-K
FEATURES             Location/Qualifiers
     source          1..6171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(101..706)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        898..1201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1207..1484
                     /label=chicken beta-actin promoter
     primer_bind     1763..1785
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1847..1866
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1908..1927
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1908..1926
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1927..1951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1967..2076
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    2205..2226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2241..2246,2247..2264,2265..2271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2279..2295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2313..2324
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2331..3008
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3053..3124
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3140..3167
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3290..3309)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3355..3410
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3409..3428)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      3498..3545
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     3558..4263
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3646..4018
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3675..4263)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(4272..4305)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4473..5060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4552..4571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5073..5867)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_recomb     join(6110..6171,1..766)
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             join(6110..6171,1..63)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
ORIGIN
        1 gtcgacaagc tctgtccgtt tgctggcaac tgcaagggac tcaatcctat ttgtaattat
       61 tgaataataa aacaattata aatgtcaaat ttgtttttta ttaacgatac aaaccaaacg
      121 caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt
      181 aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt
      241 cacataaact agacgccttg tcgtcctctt cttcgtattc cttctctttt tcatttttct
      301 cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa
      361 attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca
      421 tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt
      481 taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc
      541 cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa
      601 cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta
      661 tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca
      721 caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatg gccggccgta
      781 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctctag
      841 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      901 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      961 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1021 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1081 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1141 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1201 gcatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     1261 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     1321 ggggggggcg cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga
     1381 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     1441 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     1501 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     1561 ccgcgttact cccacaggtg agcgggcggg acggcccttc tccttcgggc tgtaattagc
     1621 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     1681 gggagggccc tttgtgcggg gggagcggct cggggctgtc cgcgggggga cggctgcctt
     1741 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1801 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1861 attgtgctgt ctcatcattt tggcaaagaa ttggatcgga ccgaaattaa tacgactcac
     1921 tataggggaa ttgtgagcgg ataacaattc cccggagtta atccgggacc tttaattcaa
     1981 cccaacacaa tatattatag ttaaataaga attattatca aatcatttgt atattaatta
     2041 aaatactata ctgtaaatta cattttattt acaatcaaag gagatatacc attgagaccg
     2101 atatcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2161 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2221 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2281 gtgagcggat aacaatttca cacatactag agaaagagga gaaatactag atggcttcct
     2341 ccgaggacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
     2401 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
     2461 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
     2521 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
     2581 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgag gacggtggtg
     2641 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
     2701 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
     2761 aagcttccac cgaacgtatg tacccggagg acggtgctct gaaaggtgaa atcaaaatgc
     2821 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
     2881 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
     2941 acaacgagga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
     3001 gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa
     3061 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3121 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtctca
     3181 gctttctagg gatccaccag tttgtgatta acctcaggtg caggctgcct atcagaaggt
     3241 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat ctttttccct
     3301 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     3361 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     3421 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     3481 catatgccca tatgtaacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     3541 ttttgctgaa agcatgcgga ggaaattctc cttgaagttt ccctggtgtt caaagtaaag
     3601 gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac gatacattgt
     3661 tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga caattgttgt
     3721 acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag agcgaaaatc
     3781 aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat agatttgtaa
     3841 aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc tggactatct
     3901 aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa tctagctttg
     3961 tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa aatattatgc
     4021 gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt aaacacgtta
     4081 aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg attatcgtcg
     4141 tcgtcccaac cctcgtcgtt agaagttgct tccgaagatg attttgccat agccacacga
     4201 cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga gctttttgga
     4261 attgcgatcg cataacttcg tatagcatac attatacgaa gttataagct cggaacgctg
     4321 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4381 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4441 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc cctgacgagc
     4501 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4561 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4621 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4681 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4741 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4801 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4861 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4921 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4981 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5041 gcagaaaaaa aggatctcaa gaagatcctt tgctaaaaca attcatccag taaaatataa
     5101 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     5161 tgttcttccc cgatatcctc cctgagcgac cggacgcaga aggcaatgtc ataccacttg
     5221 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     5281 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     5341 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcctcttc ccagttttcg
     5401 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     5461 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     5521 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     5581 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     5641 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     5701 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     5761 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     5821 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccatact cttccttttt
     5881 caatattatt gaagcattta tcagggttat tgtctcatgt ccgcgcgttt cctgcatctt
     5941 ttaatcaaat cccaagatgt gtataaacgc gccggtatgt acaggaagag gtttatacta
     6001 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     6061 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     6121 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac t
//
