LOCUS       sequence-344695-        3900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Patinios C, Creutzburg SCA, Arifah AQ, Adiego-Perez B, Gyimah EA, 
            Ingham CJ, Kengen SWM, van der Oost J, Staals RHJ
  TITLE     Streamlined CRISPR genome engineering in wild-type bacteria using 
            SIBR-Cas.
  JOURNAL   Nucleic Acids Res. 2021 Oct 6. pii: 6382381. doi: 
            10.1093/nar/gkab893.
  PUBMED    34614191
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Oct 6. pii: 6382381. doi: 10.1093/nar/gkab893."
COMMENT     Sequence Label: pSIBR001
FEATURES             Location/Qualifiers
     source          1..3900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaatttatc aagaatttgt taataaatat agtttaagta aaactctaag atttgagtta
       61 atcccacagg gtaaaacact tgaaaacata aaagcaagag gtttgatttt agatgatgag
      121 aaaagagcta aagactacaa aaaggctaaa caaataattg ataaatatca tcagtttttt
      181 atagaggaga tattaagttc ggtttgtatt agcgaagatt tattacaaaa ctattctgat
      241 gtttatttta aacttaaaaa gagtgatgat gataatctac aaaaagattt taaaagtgca
      301 aaagatacga taaagaaaca aatatctgaa tatataaagg actcagagaa atttaagaat
      361 ttgtttaatc aaaaccttat cgatgctaaa aaagggcaag agtcagattt aattctatgg
      421 ctaaagcaat ctaaggataa tggtatagaa ctatttaaag ccaatagtga tatcacagat
      481 atagatgagg cgttagaaat aatcaaatct tttaaaggtt ggacaactta ttttaagggt
      541 tttcatgaaa atagaaaaaa tgtttatagt agcaatgata ttcctacatc tattatttat
      601 aggatagtag atgataattt gcctaaattt ctagaaaata aagctaagta tgagagttta
      661 aaagacaaag ctccagaagc tataaactat gaacaaatta aaaaagattt ggcagaagag
      721 ctaacctttg atattgacta caaaacatct gaagttaatc aaagagtttt ttcacttgat
      781 gaagtttttg agatagcaaa ctttaataat tatctaaatc aaagtggtat tactaaattt
      841 aatactatta ttggtggtaa atttgtaaat ggtgaaaata caaagagaaa aggtataaat
      901 gaatatataa atctatactc acagcaaata aatgataaaa cactcaaaaa atataaaatg
      961 agtgttttat ttaagcaaat tttaagtgat acagaatcta aatcttttgt aattgataag
     1021 ttagaagatg atagtgatgt agttacaacg atgcaaagtt tttatgagca aatagcagct
     1081 tttaaaacag tagaagaaaa atctattaaa gaaacactat ctttattatt tgatgattta
     1141 aaagctcaaa aacttgattt gagtaaaatt tattttaaaa atgataaatc tcttactgat
     1201 ctatcacaac aagtttttga tgattatagt gttattggta cagcggtact agaatatata
     1261 actcaacaaa tagcacctaa aaatcttgat aaccctagta agaaagagca agaattaata
     1321 gccaaaaaaa ctgaaaaagc aaaatactta tctctagaaa ctataaagct tgccttagaa
     1381 gaatttaata agcatagaga tatagataaa cagtgtaggt ttgaagaaat acttgcaaac
     1441 tttgcggcta ttccgatgat atttgatgaa atagctcaaa acaaagacaa tttggcacag
     1501 atatctatca aatatcaaaa tcaaggtaaa aaagacctac ttcaagctag tgcggaagat
     1561 gatgttaaag ctatcaagga tcttttagat caaactaata atctcttaca taaactaaaa
     1621 atatttcata ttagtcagtc agaagataag gcaaatattt tagacaagga tgagcatttt
     1681 tatctagtat ttgaggagtg ctactttgag ctagcgaata tagtgcctct ttataacaaa
     1741 attagaaact atataactca aaagccatat agtgatgaga aatttaagct caattttgag
     1801 aactcgactt tggctaatgg ttgggataaa aataaagagc ctgacaatac ggcaatttta
     1861 tttatcaaag atgataaata ttatctgggt gtgatgaata agaaaaataa caaaatattt
     1921 gatgataaag ctatcaaaga aaataaaggc gagggttata aaaaaattgt ttataaactt
     1981 ttacctggcg caaataaaat gttacctaag gttttctttt ctgctaaatc tataaaattt
     2041 tataatccta gtgaagatat acttagaata agaaatcatt ccacacatac aaaaaatggt
     2101 agtcctcaaa aaggatatga aaaatttgag tttaatattg aagattgccg aaaatttata
     2161 gatttttata aacagtctat aagtaagcat ccggagtgga aagattttgg atttagattt
     2221 tctgatactc aaagatataa ttctatagat gaattttata gagaagttga aaatcaaggc
     2281 tacaaactaa cttttgaaaa tatatcagag agctatattg atagcgtagt taatcagggt
     2341 aaattgtacc tattccaaat ctataataaa gatttttcag cttatagcaa agggcgacca
     2401 aatctacata ctttatattg gaaagcgctg tttgatgaga gaaatcttca agatgtggtt
     2461 tataagctaa atggtgaggc agagcttttt tatcgtaaac aatcaatacc taaaaaaatc
     2521 actcacccag ctaaagaggc aatagctaat aaaaacaaag ataatcctaa aaaagagagt
     2581 gtttttgaat atgatttaat caaagataaa cgctttactg aagataagtt tttctttcac
     2641 tgtcctatta caatcaattt taaatctagt ggagctaata agtttaatga tgaaatcaat
     2701 ttattgctaa aagaaaaagc aaatgatgtt catatattaa gtatagatag aggtgaaaga
     2761 catttagctt actatacttt ggtagatggt aaaggcaata tcatcaaaca agatactttc
     2821 aacatcattg gtaatgatag aatgaaaaca aactaccatg ataagcttgc tgcaatagag
     2881 aaagataggg attcagctag gaaagactgg aaaaagataa ataacatcaa agagatgaaa
     2941 gagggctatc tatctcaggt agttcatgaa atagctaagc tagttataga gtataatgct
     3001 attgtggttt ttgaggattt aaattttgga tttaaaagag ggcgtttcaa ggtagagaag
     3061 caggtctatc aaaagttaga aaaaatgcta attgagaaac taaactatct agttttcaaa
     3121 gataatgagt ttgataaaac tgggggagtg cttagagctt atcagctaac agcacctttt
     3181 gagactttta aaaagatggg taaacaaaca ggtattatct actatgtacc agctggtttt
     3241 acttcaaaaa tttgtcctgt aactggtttt gtaaatcagt tatatcctaa gtatgaaagt
     3301 gtcagcaaat ctcaagagtt ctttagtaag tttgacaaga tttgttataa ccttgataag
     3361 ggctattttg agtttagttt tgattataaa aactttggtg acaaggctgc caaaggcaag
     3421 tggactatag ctagctttgg gagtagattg attaacttta gaaattcaga taaaaatcat
     3481 aattgggata ctcgagaagt ttatccaact aaagagttgg agaaattgct aaaagattat
     3541 tctatcgaat atgggcatgg cgaatgtatc aaagcagcta tttgcggtga gagcgacaaa
     3601 aagttttttg ctaagctaac tagtgtccta aatactatct tacaaatgcg taactcaaaa
     3661 acaggtactg agttagatta tctaatttca ccagtagcag atgtaaatgg caatttcttt
     3721 gattcgcgac aggcgccaaa aaatatgcct caagatgctg atgccaatgg tgcttatcat
     3781 attgggctaa aaggtctgat gctactaggt aggatcaaaa ataatcaaga gggcaaaaaa
     3841 ctcaatttgg ttatcaaaaa tgaagagtat tttgagttcg tgcagaatag gaataactaa
//
