LOCUS       sequence-344697-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty vector for cloning shRNA and/or miRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  TITLE     Improved vector system for knockdown
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-U6-SGIPZ
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..87
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(281..979)
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1050..1074)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1071..1274)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1100..1120)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1275..1578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(1677..1917)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(1727..1746)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1897..1917)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1944..1991
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        1983..2001
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2005..2014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2011..2385
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2865..2909)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3094..3327
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3824..3949
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3996..4629
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4797..4931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4821..4840)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4875..4894
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4972..5107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5179..5283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5284..5352,5353..6144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5502..5521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6315..6903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6804..6823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7114..7225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7214..7231
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             7332..7565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7772..8360
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7878..7889
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8287..8307
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8585..9187)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8668..8688)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(9190..9508,1..254)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9349..9368)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca
       61 acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga
      121 cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca
      181 tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg
      241 gggagggaga ggggcggaat ttgcggccgc tacttgtaca ttattcttca ccggcatctg
      301 catccggggt cttgaaggcg tgctggtact ccacgatgcc cagctcggtg ttgctgtgat
      361 cctcctccac gcggcggaag gcgaacatgg ggcccccgtt ctgcaggatg ctggggtgga
      421 tggcgctctt gaagtgcatg tggctgtcca ccacggagct gtagtagccg ccgtcgcgca
      481 ggctgaaggt gcgggtgaag ctgccatcca gatcgttatc gcccatgggg tgcaggtgct
      541 ccacggtggc gttgctgcgg atgatcttgt cggtgaagat cacgctgtcc tcggggaagc
      601 cggtgcccat caccttgaag tcgccgatca cgcggccggc ctcgtagcgg tagctgaagc
      661 tcacgtgcag cacgccgccg tcctcgtact tctcgatgcg ggtgttggtg tagccgccgt
      721 tgttgatggc gtgcaggaag gggttctcgt agccgctggg gtaggtgccg aagtggtaga
      781 agccgtagcc catcacgtgg ctcagcaggt aggggctgaa ggtcagggcg cctttggtgc
      841 tcttcatctt gttggtcatg cggccctgct cgggggtgcc ctctccgccg cccaccagct
      901 cgaactccac gccgttcagg gtgccggtga tgcggcactc gatctccatg gcgggcaggc
      961 cgctctcgtc gctctccatg gtggcagatc ctctagtaga gtcggtgtct tctatggagg
     1021 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
     1081 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     1141 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     1201 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     1261 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     1321 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     1381 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     1441 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     1501 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     1561 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     1621 gattactatt aataactaat gcatggcggt aatacgtcta gaaaaaaaga atcgatgtcc
     1681 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
     1741 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
     1801 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc
     1861 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcact
     1921 agaacctcgg acctgcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     1981 tataatacga ctcactatag gagggccacc atggccaagt tgaccagtgc cgttccggtg
     2041 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     2101 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     2161 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     2221 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     2281 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     2341 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgattccg gactagagcg
     2401 ggcccggtac taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
     2461 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     2521 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     2581 ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa tcgaatggat
     2641 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
     2701 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
     2761 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
     2821 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
     2881 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
     2941 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
     3001 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
     3061 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
     3121 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
     3181 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
     3241 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
     3301 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     3361 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     3421 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     3481 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     3541 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     3601 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     3661 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     3721 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     3781 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     3841 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     3901 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     3961 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     4021 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     4081 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     4141 ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt
     4201 atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa
     4261 agtccccagc ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg
     4321 gatgcagctc tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca
     4381 cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct
     4441 ctcctttatt ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc
     4501 caaaggtcag tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt
     4561 agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta
     4621 gcccttccaa ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa
     4681 aataattcag aggaatcaca ggtttagagt aaatgaaacc acaggtaatt ggcagtggta
     4741 atagggtatg gggtgggaag tttgggatga ttttggttag cttgagttat ccagttgatc
     4801 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     4861 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     4921 ataaacaagt tgatctcccg atccgtcgac gaagcttttt gcaaaagcct aggcctccaa
     4981 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
     5041 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
     5101 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaactcgac gtcaggtggc
     5161 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5221 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5281 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5341 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5401 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5461 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5521 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5581 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5641 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5701 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5761 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5821 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5881 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5941 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6001 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6061 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6121 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6181 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6241 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6301 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6361 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6421 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6481 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6541 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6601 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6661 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6721 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6781 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6841 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6901 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6961 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7021 gctgataccc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     7081 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     7141 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     7201 gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac aatgtcaagg
     7261 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     7321 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     7381 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     7441 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     7501 ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc
     7561 ttccagtccc ctcttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     7621 tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc
     7681 tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt
     7741 ttctaggtct cgactgcaga attaattcca ggcggggagg cggcccaaag ggagatccga
     7801 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     7861 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     7921 atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc
     7981 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     8041 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     8101 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     8161 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     8221 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     8281 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     8341 ttgtaatcca gaggttgatt gttccagacg cgtgactcaa tttaaataaa acacattagt
     8401 aacattaata tacattaaaa cacaactgga tccggttacc tcgagaagag ctatgaataa
     8461 aaagaaatat attttcttca gaagagaatt ctgttgggtt aacctgaaga agtaatccca
     8521 gcaagtgttt ccaagatgtg caggcaacga ttctgtaaag tactgaagcc tcattcaaac
     8581 aaactcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
     8641 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
     8701 tctccaggaa ggcgggcacc ccagcgcgct cggccgcctc cactccgggg agcacgacgg
     8761 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
     8821 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
     8881 gggaaccgct caactcggcc atgcgcgagc cgatctcggc gaacaccgcc cccgcttcga
     8941 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
     9001 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     9061 ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     9121 tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     9181 tggccatggt attatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
     9241 agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca
     9301 gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag
     9361 gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc
     9421 gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt
     9481 ggggggttcc gctgcctgca aagggtcg
//
