LOCUS       sequence-344698-        6156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pOPIN-GG expression vector compatible with C-terminal tagging, 
            Kanamycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6156)
  TITLE     Banfield lab plasmids
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPGC-K
FEATURES             Location/Qualifiers
     source          1..6156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(101..706)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        898..1201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1747..1769
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1831..1850
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1892..1911
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1892..1910
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1911..1935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1951..2060
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    2190..2211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2226..2231,2232..2249,2250..2256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2264..2280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2298..2309
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2316..2993
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3038..3109
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3125..3152
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3275..3294)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3340..3395
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3394..3413)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      3483..3530
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     3543..4248
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           3631..4003
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3660..4248)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(4257..4290)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4458..5045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4537..4556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5058..5852)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_recomb     join(6095..6156,1..766)
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             join(6095..6156,1..63)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
ORIGIN
        1 gtcgacaagc tctgtccgtt tgctggcaac tgcaagggac tcaatcctat ttgtaattat
       61 tgaataataa aacaattata aatgtcaaat ttgtttttta ttaacgatac aaaccaaacg
      121 caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt
      181 aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata attttatttt
      241 cacataaact agacgccttg tcgtcctctt cttcgtattc cttctctttt tcatttttct
      301 cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa
      361 attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca
      421 tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt
      481 taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc
      541 cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa
      601 cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta
      661 tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca
      721 caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatg gccggccgta
      781 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctctag
      841 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      901 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      961 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1021 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1081 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1141 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1201 gcatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     1261 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     1321 gggggggggg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1381 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1441 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1501 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1561 ggtgagcggg cgggacggcc cttctccttc gggctgtaat tagcgcttgg tttaatgacg
     1621 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1681 cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     1741 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     1801 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     1861 attttggcaa agaattggat cggaccgaaa ttaatacgac tcactatagg ggaattgtga
     1921 gcggataaca attccccgga gttaatccgg gacctttaat tcaacccaac acaatatatt
     1981 atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa
     2041 attacatttt atttacaatc aaaggagata tacaatgtga gaccgatatc aatacgcaaa
     2101 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     2161 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2221 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2281 tttcacacat actagagaaa gaggagaaat actagatggc ttcctccgag gacgttatca
     2341 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     2401 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     2461 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     2521 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     2581 aaggtttcaa atgggaacgt gttatgaact tcgaggacgg tggtgttgtt accgttaccc
     2641 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     2701 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     2761 gtatgtaccc ggaggacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     2821 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     2881 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaggactaca
     2941 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     3001 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     3061 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3121 cactggctca ccttcgggtg ggcctttctg cgtttatagg tctcagcttt ctagggatcc
     3181 accagtttgt gattaacctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     3241 caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc aaaaattatg
     3301 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     3361 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     3421 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgt
     3481 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagcat
     3541 gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac
     3601 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat
     3661 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat
     3721 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg
     3781 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta
     3841 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc
     3901 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt
     3961 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt
     4021 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca
     4081 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg
     4141 tcgttagaag ttgcttccga agatgatttt gccatagcca cacgacgcct attaattgtg
     4201 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc gatcgcataa
     4261 cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc ggtcgttcgg
     4321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4441 gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac aaaaatcgac
     4501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5041 ctcaagaaga tcctttgcta aaacaattca tccagtaaaa tataatattt tattttctcc
     5101 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     5161 tcctccctga gcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     5221 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     5281 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     5341 agctcgcgcg gatctttaaa tggagtgtcc tcttcccagt tttcgcaatc cacatcggcc
     5401 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     5461 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     5521 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     5581 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     5641 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     5701 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     5761 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
     5821 ttcaattccg gtgatattct cattttagcc atactcttcc tttttcaata ttattgaagc
     5881 atttatcagg gttattgtct catgtccgcg cgtttcctgc atcttttaat caaatcccaa
     5941 gatgtgtata aacgcgccgg tatgtacagg aagaggttta tactaaactg ttacattgca
     6001 aacgtggttt cgtgtgccaa gtgtgaaaac cgatgtttaa tcaaggctct gacgcatttc
     6061 tacaaccacg actccaagtg tgtgggtgaa gtcatgcatc ttttaatcaa atcccaagat
     6121 gtgtataaac caccaaactg ccaaaaaatg aaaact
//
