LOCUS       sequence-34470-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Tsai WW, Wang Z, Yiu TT, Akdemir KC, Xia W, Winter S, Tsai CY, Shi 
            X, Schwarzer D, Plunkett W, Aronow B, Gozani O, Fischle W, Hung MC, 
            Patel DJ, Barton MC
  TITLE     TRIM24 links a non-canonical histone signature to breast cancer.
  JOURNAL   Nature. 2010 Dec 16. 468(7326):927-32.
  PUBMED    21164480
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Dec 16. 468(7326):927-32."
COMMENT     Sequence Label: Flag-TRIM24-C840W shRescue
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             124..147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccacc atggcagact acaaggacga cgatgacaag
      121 gcagactaca aggacgacga tgacaagggt acctctagaa gatctaggac aatggaggtg
      181 gcggtggaga aggcggtggc ggcggcggca gcggcctcgg ctgcggcctc cggggggccc
      241 tcggcggcgc cgagcgggga gaacgaggcc gagagtcggc agggcccgga ctcggagcgc
      301 ggcggcgagg cggcccggct caacctgttg gacacttgcg ccgtgtgcca ccagaacatc
      361 cagagccggg cgcccaagct gctgccctgc ctgcactctt tctgccagcg ctgcctgccc
      421 gcgccccagc gctacctcat gctgcccgcg cccatgctgg gctcggccga gaccccgcca
      481 cccgtccctg cccccggctc gccggtcagc ggctcgtcgc cgttcgccac ccaagttgga
      541 gtcattcgtt gcccagtttg cagccaagaa tgtgcagaga gacacatcat agataacttt
      601 tttgtgaagg acactactga ggttcccagc agtacagtag aaaagtcaaa tcaggtatgt
      661 acaagctgtg aggacaacgc agaagccaat gggttttgtg tagagtgtgt tgaatggctc
      721 tgcaagacgt gtatcagagc tcatcanagg gtaaagttca caaaagacca cactgtcaga
      781 cagaaagagg aagtatctcc ag
//
