LOCUS       sequence-344708-         288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Patinios C, Creutzburg SCA, Arifah AQ, Adiego-Perez B, Gyimah EA, 
            Ingham CJ, Kengen SWM, van der Oost J, Staals RHJ
  TITLE     Streamlined CRISPR genome engineering in wild-type bacteria using 
            SIBR-Cas.
  JOURNAL   Nucleic Acids Res. 2021 Oct 6. pii: 6382381. doi: 
            10.1093/nar/gkab893.
  PUBMED    34614191
REFERENCE   2  (bases 1 to 288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Oct 6. pii: 6382381. doi: 10.1093/nar/gkab893."
COMMENT     Sequence Label: pSIBR002
FEATURES             Location/Qualifiers
     source          1..288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcctcgggtt aattgaggcc tgagtataag gtgacttata cttgtaatct atctaaacgg
       61 ggaacctctc tagtagacaa tcccgtgcta aattgatacc agcatcgtct tgatgccctt
      121 ggcagcataa atgcctaacg actatccctt tggggagtag ggtcaagtga ctcgaaacga
      181 tagacaactt gctttaacaa gttggagata tagtctgctc tgcatggtga catgcagctg
      241 gatataattc cggggtaaga ttaacgacct tatctgaaca taatgcta
//
