LOCUS       sequence-344710-         111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 111)
  AUTHORS   Patinios C, Creutzburg SCA, Arifah AQ, Adiego-Perez B, Gyimah EA, 
            Ingham CJ, Kengen SWM, van der Oost J, Staals RHJ
  TITLE     Streamlined CRISPR genome engineering in wild-type bacteria using 
            SIBR-Cas.
  JOURNAL   Nucleic Acids Res. 2021 Oct 6. pii: 6382381. doi: 
            10.1093/nar/gkab893.
  PUBMED    34614191
REFERENCE   2  (bases 1 to 111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Oct 6. pii: 6382381. doi: 10.1093/nar/gkab893."
COMMENT     Sequence Label: pSIBR002
FEATURES             Location/Qualifiers
     source          1..111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtctaagaac tttaaataat ttctactgtt gtagatatgt cttcctgagt gacgagcaac
       61 gagactggaa gacatgtcta agaactttaa ataatttcta ctgttgtaga t
//
