LOCUS       sequence-344725-        3903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of FnCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Patinios C, Creutzburg SCA, Arifah AQ, Adiego-Perez B, Gyimah EA, 
            Ingham CJ, Kengen SWM, van der Oost J, Staals RHJ
  TITLE     Streamlined CRISPR genome engineering in wild-type bacteria using 
            SIBR-Cas.
  JOURNAL   Nucleic Acids Res. 2021 Oct 6. pii: 6382381. doi: 
            10.1093/nar/gkab893.
  PUBMED    34614191
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Oct 6. pii: 6382381. doi: 10.1093/nar/gkab893."
COMMENT     Sequence Label: pSIBR005
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag
       61 ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat
      121 gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt
      181 tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct
      241 gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt
      301 gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag
      361 aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta
      421 tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca
      481 gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag
      541 ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt
      601 tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt
      661 ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa
      721 gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt
      781 gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa
      841 tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata
      901 aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa
      961 atgagtgttt tatttaagca aattttaagt gatacagaat ctaaatcttt tgtaattgat
     1021 aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca
     1081 gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat
     1141 ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact
     1201 gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat
     1261 ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta
     1321 atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta
     1381 gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca
     1441 aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca
     1501 cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa
     1561 gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta
     1621 aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat
     1681 ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac
     1741 aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt
     1801 gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt
     1861 ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata
     1921 tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa
     1981 cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa
     2041 ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat
     2101 ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt
     2161 atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga
     2221 ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa
     2281 ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag
     2341 ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga
     2401 ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg
     2461 gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa
     2521 atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag
     2581 agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt
     2641 cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc
     2701 aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa
     2761 agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact
     2821 ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata
     2881 gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg
     2941 aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat agagtataat
     3001 gctattgtgg tttttgagga tttaaatttt ggatttaaaa gagggcgttt caaggtagag
     3061 aagcaggtct atcaaaagtt agaaaaaatg ctaattgaga aactaaacta tctagttttc
     3121 aaagataatg agtttgataa aactggggga gtgcttagag cttatcagct aacagcacct
     3181 tttgagactt ttaaaaagat gggtaaacaa acaggtatta tctactatgt accagctggt
     3241 tttacttcaa aaatttgtcc tgtaactggt tttgtaaatc agttatatcc taagtatgaa
     3301 agtgtcagca aatctcaaga gttctttagt aagtttgaca agatttgtta taaccttgat
     3361 aagggctatt ttgagtttag ttttgattat aaaaactttg gtgacaaggc tgccaaaggc
     3421 aagtggacta tagctagctt tgggagtaga ttgattaact ttagaaattc agataaaaat
     3481 cataattggg atactcgaga agtttatcca actaaagagt tggagaaatt gctaaaagat
     3541 tattctatcg aatatgggca tggcgaatgt atcaaagcag ctatttgcgg tgagagcgac
     3601 aaaaagtttt ttgctaagct aactagtgtc ctaaatacta tcttacaaat gcgtaactca
     3661 aaaacaggta ctgagttaga ttatctaatt tcaccagtag cagatgtaaa tggcaatttc
     3721 tttgattcgc gacaggcgcc aaaaaatatg cctcaagatg ctgatgccaa tggtgcttat
     3781 catattgggc taaaaggtct gatgctacta ggtaggatca aaaataatca agagggcaaa
     3841 aaactcaatt tggttatcaa aaatgaagag tattttgagt tcgtgcagaa taggaataac
     3901 taa
//
