LOCUS       sequence-344732-        2346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag module carrying Twin Streptavidin 
            uncleavable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2346)
  TITLE     Banfield lab plasmids
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30022
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(160..951)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1173..1277)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1345..1363
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(1402..1407,1408..1431,1432..1467,1468..1491)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     primer_bind     complement(1576..1593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1747..2335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1827..1846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
       61 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      121 atcaatctaa agtatatatg agtaaacttg gtctgacagt tatttgccga ctaccttggt
      181 gatctcgcct tttacgtaat ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
      241 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
      301 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
      361 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
      421 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
      481 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
      541 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
      601 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
      661 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
      721 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
      781 ccttacggtc accgtaacca gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac
      841 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
      901 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa
      961 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     1021 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     1081 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     1141 gtcaaggttc tggaccagtt gcgtgagcgc atactcttcc tttttcaata ttattgaagc
     1201 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1261 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     1321 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tccactgaag
     1381 agccactgtg gtctcaccat gtcagcttgg tcccaccctc aattcgagaa aggaggcggt
     1441 tcaggtggcg gatcgggggg ttctgcatgg tcgcatcctc agtttgaaaa gtcaggaatg
     1501 tgagaccacg agtgattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1561 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2341 tccttt
//
