LOCUS       sequence-344745-       11726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A)
            with miniTn7T method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, 
            Carothers JM, Zalatan JG
  TITLE     Portable bacterial CRISPR transcriptional activation enables 
            metabolic engineering in Pseudomonas putida.
  JOURNAL   Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002.
  PUBMED    33930546
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002."
COMMENT     Sequence Label: pPPC005
FEATURES             Location/Qualifiers
     source          1..11726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..634
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    complement(764..811)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(857..943)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(1046..1140)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1168..1184)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1169..1185)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1599..1618)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1718..1740
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1778..1796)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1864..1968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1969..2037,2038..2829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2187..2206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3000..3588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3489..3508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3820..3928
                     /note="incP origin of transfer"
     mobile_element  4087..4252
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     RBS             5020..5031
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(5041..5075)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     terminator      complement(5128..5155)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(5171..5242)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5266..6852,6853..6855,6856..9342,9343..9345,
                     9346..9372))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(9432..9512)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             10412..11377
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     terminator      11402..11488
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(11522..11569)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(11610..11615,11616..11632,11633..11638)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gcttgttatg actgtttttt tgtacagtct atgcctcggg catccaagca gcaagcgcgt
       61 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagcaacga
      121 tgttacgcag cagggcagtc gccctaaaac aaagttaggt ggctcaagta tgggcatcat
      181 tcgcacatgt aggctcggcc ctgaccaagt caaatccatg cgggctgctc ttgatctttt
      241 cggtcgtgag ttcggagacg tagccaccta ctcccaacat cagccggact ccgattacct
      301 cgggaacttg ctccgtagta agacattcat cgcgcttgct gccttcgacc aagaagcggt
      361 tgttggcgct ctcgcggctt acgttctgcc caagtttgag cagccgcgta gtgagatcta
      421 tatctatgat ctcgcagtct ccggcgagca ccggaggcag ggcattgcca ccgcgctcat
      481 caatctcctc aagcatgagg ccaacgcgct tggtgcttat gtgatctacg tgcaagcaga
      541 ttacggtgac gatcccgcag tggctctcta tacaaagttg ggcatacggg aagaagtgat
      601 gcactttgat atcgacccaa gtaccgccac ctaacaattc gttcaagccg agatcggctt
      661 cccggccgcg gagttgttcg gtaaattgtc acaacgccgc ggccaattcg atctagaatt
      721 ccattgagta agtttttaag cacatcagct tcaaaagcgc tctgaagttc ctatactttc
      781 tagagaatag gaacttcgga ataggtactt caagatcccc aattcgatcg tccgggccgc
      841 aagctcctag cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
      901 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctc aagctagaga
      961 gtcattaccc caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     1021 tgccactcat cgcagtctag cttggattct caccaataaa aaacgcccgg cggcaaccga
     1081 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     1141 caagctcagc taattaagct agcttatcga taccgtcgac ctcgaacccc acgcccctct
     1201 ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa
     1261 gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta
     1321 gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc
     1381 gcccacacct gcttacgcag ggcatccatt tattactcaa ccgtaaccga ttttgccagg
     1441 ttacgcggct ggtcctctag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1501 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     1561 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     1621 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1681 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1741 ttttcaccgt cgtcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     1801 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1861 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1921 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1981 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2041 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2101 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2161 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2221 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2281 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2341 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2401 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2461 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2521 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2581 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2641 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2701 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2761 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2821 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2881 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactc
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcagggg attccttaag gtatactttc cgctgcataa ccctgcttcg gggtcattat
     3721 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt
     3781 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg
     3841 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa
     3901 cgggaatcct gctctgcgag gctggccgat aagctctgat accgctcgcc gcagccgaac
     3961 gaccgagcgc agcgggtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     4021 tctccccgcg cgttggccga ttcattaatg cagcgcccaa acataacagg aagaaaaatg
     4081 ccccgctgtg ggcggacaaa atagttggga actgggaggg gtggaaatgg agtttttaag
     4141 gattatttag ggaagagtga caaaatagat gggaactggg tgtagcgtcg taagctaata
     4201 cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg ttggcctgca
     4261 aggccttcgc gaggcgaaaa aaccccgccg aagcggggtt ttttgcgtgg cggccgctta
     4321 caggcggtgg cgataatccg cgggagtgcg atcaaactgc cgcgcgaaaa cgcgggagaa
     4381 ggtctgctgc gagacataac ccaggtccat tgcgatatca aaaatcggac gctcggtggt
     4441 gcgcaactca acggcggcca gtaacaggcg gcgttggcga atgtaatcgc caagcgtctg
     4501 atgcgtcacc gtgcggaaca ttcgttgcaa gtaccacttt gaatagcctg atttttttgc
     4561 gactacatca atgttaagcg gctggtcaat atgctcgtca atccatgcga taagatcctg
     4621 aataattttc tgatgggaca tgctacctcc gccaccgtag atgccggagt ttgctgcgat
     4681 tgctgaggga atcgggtttc catcttttag gagaccttgc attgccttaa caataagctc
     4741 gcagtcggaa ttcgtggcga aaattggaat ggttagttcc atatttaagt acgaacgcca
     4801 ggcgccttta ggcacctcga ctttgatggt gtatttgcga ttctgcgcag agctctgacg
     4861 aacgctacag gttactttgt aagcctgtga acgcgagtta gagctgatcc attcagcgat
     4921 cccgttagcg aagttgcttg gggcgacagt cacgtcgcca gttccgccat tgtcgacgag
     4981 aacgaactga gtaaagttag aagcgggccc catggtacct ttctcctctt taatgaattc
     5041 gctagcataa tacctagggc tgagctagcc gtaaactgca gattcaccac cctgaattga
     5101 ctctcttccg ggcgctctag gtataaacgc agaaaggccc acccgaaggt gagccagtgt
     5161 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     5221 actgagcctt tcgttttatt tgatgcctgg agatccttac tcgagttagt cacctcctag
     5281 ctgactcaaa tcaatgcgtg tttcataaag accagtgatg gattgatgga taagagtggc
     5341 atctaaaact tcttttgtag acgtatatcg tttacgatca attgttgtat caaaatattt
     5401 aaaagcagcg ggagctccaa gattcgtcaa cgtaaataaa tgaataatat tttctgcttg
     5461 ttcacgtatt ggtttgtctc tatgtttgtt atatgcacta agaactttat ctaaattggc
     5521 atctgctaaa ataacacgct tagaaaattc actgatttgc tcaataatct catctaaata
     5581 atgcttatgc tgctccacaa acaattgttt ttgttcgtta tcttctggac tacccttcaa
     5641 cttttcataa tgactagcta aatataaaaa attcacatat ttgcttggca gagccagctc
     5701 atttcctttt tgtaattctc cggcactagc cagcatccgt ttacgaccgt tttctaactc
     5761 aaaaagacta tatttaggta gtttaatgat taagtctttt ttaacttcct tatatccttt
     5821 agcttctaaa aagtcaatcg gatttttttc aaaggaactt ctttccataa ttgtgatccc
     5881 tagtaactct ttaacggatt ttaacttctt cgatttccct ttttccacct tagcaaccac
     5941 taggactgaa taagctaccg ttggactatc aaaaccacca tatttttttg gatcccagtc
     6001 ttttttacga gcaataagct tgtccgaatt tctttttggt aaaattgact ccttggagaa
     6061 tccgcctgtc tgtacttctg ttttcttgac aatattgact tggggcatgg acaatacttt
     6121 gcgcactgtg gcaaaatctc gccctttatc ccagacaatt tctccagttt ccccattagt
     6181 ttcgattaga gggcgtttgc gaatctctcc atttgcaagt gtaatttctg ttttgaagaa
     6241 gttcatgata ttagagtaaa agaaatattt tgcggttgct ttgcctattt cttgctcaga
     6301 cttagcaatc attttacgaa catcataaac tttataatca ccatagacaa actccgattc
     6361 aagttttgga tatttcttaa tcaaagcagt tccaacgacg gcatttagat acgcatcatg
     6421 ggcatgatgg taattgttaa tctcacgtac tttatagaat tggaaatctt ttcggaagtc
     6481 agaaactaat ttagatttta aggtaatcac tttaacctct cgaataagtt tatcattttc
     6541 atcgtattta gtattcatgc gactatccaa aatttgtgcc acatgcttag tgatttggcg
     6601 agtttcaacc aattggcgtt tgataaaacc agctttatca agttcactca aacctccacg
     6661 ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg
     6721 tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc
     6781 acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact
     6841 ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta attcttggtc
     6901 cacatacatg tctcttccat tttggagata atagagatag agcttttcat tttgcaattg
     6961 agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc
     7021 gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg
     7081 tgccatttca ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc
     7141 aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc
     7201 aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt
     7261 aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga
     7321 tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt
     7381 tcgagacaaa cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc
     7441 atcaaagagg tgagcatatg ttttaagtct ttcctcaatc atctccctat cttcaaataa
     7501 ggtcaatgtt aaaacaatat cctctaagat atcttcattt tcttcattat ccaaaaaatc
     7561 tttatcttta ataattttta gcaaatcatg gtaggtacct aatgaagcat taaatctatc
     7621 ttcaactcct gaaatttcaa cactatcaaa acattctatt tttttgaaat aatcttcttt
     7681 taattgctta acggttactt ttcgatttgt tttgaagagt aaatcaacaa tggctttctt
     7741 ctgttcacct gaaagaaatg ctggttttcg cattccttca gtaacatatt tgacctttgt
     7801 caattcgtta taaaccgtaa aatactcata aagcaaacta tgttttggta gtactttttc
     7861 atttggaaga tttttatcaa agtttgtcat gcgttcaata aatgattgag ctgaagcacc
     7921 tttatcgaca acttcttcaa aattccatgg ggtaattgtt tcttcagact tccgagtcat
     7981 ccatgcaaaa cgactattgc cacgcgccaa tggaccaaca taataaggaa ttcgaaaagt
     8041 caagattttt tcaatcttct cacgattgtc ttttaaaaat ggataaaagt cttcttgtct
     8101 tctcaaaata gcatgcagct cacccaagtg aatttgatgg ggaatagagc cgttgtcaaa
     8161 ggtccgttgc ttgcgcagca aatcttcacg atttagtttc accaataatt cctcagtacc
     8221 atccattttt tctaaaattg gtttgataaa tttataaaat tcttcttggc tagctccccc
     8281 atcaatataa cctgcatatc cgttttttga ttgatcaaaa aagatttctt tatacttttc
     8341 tggaagttgt tgtcgaacta aagcttttaa aagagtcaag tcttgatgat gttcatcgta
     8401 gcgtttaatc attgaagctg ataggggagc cttagttatt tcagtattta ctcttaggat
     8461 atctgaaagt aaaatagcat ctgataaatt cttagctgcc aaaaacaaat cagcatattg
     8521 atctccaatt tgcgccaata aattatctaa atcatcatcg taagtatctt ttgaaagctg
     8581 taatttagca tcttctgcca aatcaaaatt tgatttaaaa ttaggggtca aacccaatga
     8641 caaagcaatg agattcccaa ataagccatt tttcttctca ccggggagct gagcaatgag
     8701 attttctaat cgtcttgatt tactcaatcg tgcagaaaga atcgctttag catctactcc
     8761 acttgcgtta atagggtttt cttcaaataa ttgattgtag gtttgtacca actggataaa
     8821 tagtttgtcc acatcactat tatcaggatt taaatctccc tcaatcaaaa aatgaccacg
     8881 aaacttaatc atatgcgcta aggccaaata gattaagcgc aaatccgctt tatcagtaga
     8941 atctaccaat ttttttcgca gatgatagat agttggatat ttctcatgat aagcaacttc
     9001 atctactata tttccaaaaa taggatgacg ttcatgcttc ttgtcttctt ccaccaaaaa
     9061 agactcttca agtcgatgaa agaaactatc atctactttc gccatctcat ttgaaaaaat
     9121 ctcctgtaga taacaaatac gattcttccg acgtgtatac cttctacgag ctgtccgttt
     9181 gagacgagtc gcttccgctg tctctccact gtcaaataaa agagccccta taagattttt
     9241 tttgatactg tggcggtctg tatttcccag aaccttgaac tttttagacg gaaccttata
     9301 ttcatcagtg atcaccgccc atccgacgct atttgtgccg atagctaagc ctattgagta
     9361 tttcttatcc atatgttttt cctcctaacc gcggcctagg catggtcatg actccattat
     9421 tattgtttct gttgcataaa gcctaagggg taggccttac tagagatagc cattttttgc
     9481 actcctgtat ccgcttcttg caaggctgga cttatcccta tcaaaccgga cactgcactt
     9541 tatgctggtt atgctggctg catggggcat tagttgggga gccagatttg tcatggagca
     9601 ggccgttctg ctttatggat caggaaaaaa ctatttgttc ttcagtcatg gtactgttct
     9661 gatgtacctg ctgtgtgttt tcctggtata ccgccgttgg atagctccgc taccggtcgt
     9721 tggtcagatc aagctaattc ccatgcatgg tgaaaacggg ggcgaagaag ttgtccatat
     9781 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     9841 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     9901 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     9961 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
    10021 gctcaccgtc tttcattgcc atacgtaatt acggatgagc attcatcagg cgggcaagaa
    10081 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
    10141 taatatccag cagatcccct ttatccgcca attcgtcgcc ccatgccccg acagcacgaa
    10201 cttctggtgt tctcgcttct taaaaagaac gtcttcgttc tgcttggcgt tatttttgct
    10261 tggaaaagtg gtcactgatt gcaaaaagga tggcgcaacg tggcaatggg ggtaacccgt
    10321 atacgcatca cgtggagatg cattttcatc gacttggcgc ctttctacat cacaccaagc
    10381 agcccacatt aaaataagag aaccgtgaac tatggatttt tgcttattga acgagaaaag
    10441 tcagatcttc gtccacgccg agccctatgc agtctccgat tatgttaacc agtatgtcgg
    10501 tacgcactct attcgcctgc ccaagggcgg gcgcccggca ggcaggctgc accacagaat
    10561 cttcggatgc ctcgatctgt gtcgaatcag ctacggcggt agcgtgaggg taatctcgcc
    10621 tggattagaa acctgttatc atctgcaaat aatactcaaa ggccattgcc tgtggcgtgg
    10681 ccatggccag gagcactatt ttgcgccggg cgaactattg ctgctcaatc cggatgacca
    10741 agccgacctg acctattcag aagattgcga gaaatttatc gttaaattgc cctcagtggt
    10801 ccttgatcgg gcgtgcagtg acaacaattg gcacaagccg agggagggta tccgtttcgc
    10861 cgcgcgacac aatctccagc aactcgatgg ctttatcaat ctactcgggt tagtttgtga
    10921 cgaagcggaa catacaaagt cgatgcctcg ggtccaagag cactatgcgg ggatcatcgc
    10981 ttccaagctg ctcgaaatgc tgggcagcaa tgtcagccgt gaaattttca gcaaaggtaa
    11041 cccgtctttc gagcgagtcg ttcaattcat tgaggagaat ctcaaacgga atatcagcct
    11101 tgagcggtta gcggagctgg cgatgatgag tccacgctcg ctctacaatt tgttcgagaa
    11161 gcacgccggc accacgccga agaactacat ccgcaaccgc aagctcgaaa gcatccgcgc
    11221 ctgcttgaac gatcccagtg ccaatgtgcg tagtataact gagatagccc tagactacgg
    11281 cttcttacat ttgggacgct tcgctgaaaa ctataggagc gcgttcggcg agttgccttc
    11341 cgacaccctg cgtcaatgca aaaaggaagt ggcttgatta cgaacgttta attaaaggca
    11401 tcaaataaaa cgaaaagctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
    11461 ggtgaacgct ctcctgagta ggacaaatca tgcatgatcg aattagcttc aaaagcgctc
    11521 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca agatcccctg
    11581 attccctttg tcaacagcaa tggatcgaat tgacataagc ctgttcggtt cgtaaactgt
    11641 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
    11701 gtaacggcgc agtggcggtt ttcatg
//
