LOCUS       sequence-344748-       11210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and 
            hAAVS1 scRNA on pBBR1-KmR plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, 
            Carothers JM, Zalatan JG
  TITLE     Portable bacterial CRISPR transcriptional activation enables 
            metabolic engineering in Pseudomonas putida.
  JOURNAL   Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002.
  PUBMED    33930546
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 
            10.1016/j.ymben.2021.04.002."
COMMENT     Sequence Label: pPPC010.AAV
FEATURES             Location/Qualifiers
     source          1..11210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1826..2620
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1880..1899)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2490..2509
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     RBS             3661..3672
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(3682..3716)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     terminator      complement(3769..3796)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3812..3883)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(3907..5493,5494..5496,5497..7983,7984..7986,
                     7987..8013))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        8309..8387
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     protein_bind    8663..8684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8699..8704,8705..8722,8723..8729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8737..8753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8742..8764
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8761..8777
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8761..8777
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8796..8816
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8798..8816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(8861..8907)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     9060..9077
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(9133..9150)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9166..9195)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(9218..9236)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(9320..9354)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(9368..9387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9369..9387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9397..9413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9406..9428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(10122..10784)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      join(10785..11210,1..346)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 cgcccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag
       61 ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag
      121 cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg
      181 ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc
      241 ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca
      301 gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc
      361 cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca
      421 agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg
      481 agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc
      541 cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg cgatgcaaga
      601 agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga
      661 cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc agcagcaccg
      721 atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt
      781 tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc agccaagaac
      841 agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac ggggcggatc
      901 gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg
      961 tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac aaagcgcaga
     1021 tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg caacggggca
     1081 tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc
     1141 caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc
     1201 agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa gccgtggccg
     1261 accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc gccgcaggag
     1321 cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac cttcgggagc
     1381 gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag gccaaggccg
     1441 ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa gtccagcgcc
     1501 agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa aagtgccacc
     1561 tgggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     1621 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     1681 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     1741 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca
     1801 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     1861 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     1921 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     1981 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     2041 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     2101 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     2161 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     2221 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     2281 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     2341 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     2401 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     2461 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     2521 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     2581 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     2641 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     2701 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     2761 ggggatctca tgctggagtt cttcgcccac ccccatgggc aaatattata cgcaaggcga
     2821 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     2881 cggcagaatg cttaatgaat tacaacagtt tttagcgaaa aaaccccgcc gaagcggggt
     2941 tttttgcgtg gcggccgctt acaggcggtg gcgataatcc gcgggagtgc gatcaaactg
     3001 ccgcgcgaaa acgcgggaga aggtctgctg cgagacataa cccaggtcca ttgcgatatc
     3061 aaaaatcgga cgctcggtgg tgcgcaactc aacggcggcc agtaacaggc ggcgttggcg
     3121 aatgtaatcg ccaagcgtct gatgcgtcac cgtgcggaac attcgttgca agtaccactt
     3181 tgaatagcct gatttttttg cgactacatc aatgttaagc ggctggtcaa tatgctcgtc
     3241 aatccatgcg ataagatcct gaataatttt ctgatgggac atgctacctc cgccaccgta
     3301 gatgccggag tttgctgcga ttgctgaggg aatcgggttt ccatctttta ggagaccttg
     3361 cattgcctta acaataagct cgcagtcgga attcgtggcg aaaattggaa tggttagttc
     3421 catatttaag tacgaacgcc aggcgccttt aggcacctcg actttgatgg tgtatttgcg
     3481 attctgcgca gagctctgac gaacgctaca ggttactttg taagcctgtg aacgcgagtt
     3541 agagctgatc cattcagcga tcccgttagc gaagttgctt ggggcgacag tcacgtcgcc
     3601 agttccgcca ttgtcgacga gaacgaactg agtaaagtta gaagcgggcc ccatggtacc
     3661 tttctcctct ttaatgaatt cgctagcata atacctaggg ctgagctagc cgtaaactgc
     3721 agattcacca ccctgaattg actctcttcc gggcgctcta ggtataaacg cagaaaggcc
     3781 cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa caacagataa
     3841 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gagatcctta
     3901 ctcgagttag tcacctccta gctgactcaa atcaatgcgt gtttcataaa gaccagtgat
     3961 ggattgatgg ataagagtgg catctaaaac ttcttttgta gacgtatatc gtttacgatc
     4021 aattgttgta tcaaaatatt taaaagcagc gggagctcca agattcgtca acgtaaataa
     4081 atgaataata ttttctgctt gttcacgtat tggtttgtct ctatgtttgt tatatgcact
     4141 aagaacttta tctaaattgg catctgctaa aataacacgc ttagaaaatt cactgatttg
     4201 ctcaataatc tcatctaaat aatgcttatg ctgctccaca aacaattgtt tttgttcgtt
     4261 atcttctgga ctacccttca acttttcata atgactagct aaatataaaa aattcacata
     4321 tttgcttggc agagccagct catttccttt ttgtaattct ccggcactag ccagcatccg
     4381 tttacgaccg ttttctaact caaaaagact atatttaggt agtttaatga ttaagtcttt
     4441 tttaacttcc ttatatcctt tagcttctaa aaagtcaatc ggattttttt caaaggaact
     4501 tctttccata attgtgatcc ctagtaactc tttaacggat tttaacttct tcgatttccc
     4561 tttttccacc ttagcaacca ctaggactga ataagctacc gttggactat caaaaccacc
     4621 atattttttt ggatcccagt cttttttacg agcaataagc ttgtccgaat ttctttttgg
     4681 taaaattgac tccttggaga atccgcctgt ctgtacttct gttttcttga caatattgac
     4741 ttggggcatg gacaatactt tgcgcactgt ggcaaaatct cgccctttat cccagacaat
     4801 ttctccagtt tccccattag tttcgattag agggcgtttg cgaatctctc catttgcaag
     4861 tgtaatttct gttttgaaga agttcatgat attagagtaa aagaaatatt ttgcggttgc
     4921 tttgcctatt tcttgctcag acttagcaat cattttacga acatcataaa ctttataatc
     4981 accatagaca aactccgatt caagttttgg atatttctta atcaaagcag ttccaacgac
     5041 ggcatttaga tacgcatcat gggcatgatg gtaattgtta atctcacgta ctttatagaa
     5101 ttggaaatct tttcggaagt cagaaactaa tttagatttt aaggtaatca ctttaacctc
     5161 tcgaataagt ttatcatttt catcgtattt agtattcatg cgactatcca aaatttgtgc
     5221 cacatgctta gtgatttggc gagtttcaac caattggcgt ttgataaaac cagctttatc
     5281 aagttcactc aaacctccac gttcagcttt cgttaaatta tcaaacttac gttgagtgat
     5341 taacttggcg tttagaagtt gtctccaata gtttttcatc tttttgacta cttcttcact
     5401 tggaacgtta tccgatttac cacgattttt atcagaacgc gttaagacct tattgtctat
     5461 tgaatcgtct ttaaggaaac tttgtggaac aatggcatcg acatcataat cacttaaacg
     5521 attaatatct aattcttggt ccacatacat gtctcttcca ttttggagat aatagagata
     5581 gagcttttca ttttgcaatt gagtattttc aacaggatgc tctttaagaa tctgacttcc
     5641 taattctttg ataccttctt cgattcgttt catacgctct cgcgaatttt tctggccctt
     5701 ttgagttgtc tgattttcac gtgccatttc aataacgata ttttctggct tatgccgccc
     5761 cattactttg accaattcat caacaacttt tacagtctgt aaaatacctt ttttaatagc
     5821 agggctacca gctaaatttg caatatgttc atgtaaacta tcgccttgtc cagacacttg
     5881 tgctttttga atgtcttctt taaatgtcaa actatcatca tggatcagct gcataaaatt
     5941 gcgattggca aaaccatctg atttcaaaaa atctaatatt gttttgccag attgcttatc
     6001 cctaatacca ttaatcaatt ttcgagacaa acgtccccaa ccagtataac ggcgacgttt
     6061 aagctgtttc atcaccttat catcaaagag gtgagcatat gttttaagtc tttcctcaat
     6121 catctcccta tcttcaaata aggtcaatgt taaaacaata tcctctaaga tatcttcatt
     6181 ttcttcatta tccaaaaaat ctttatcttt aataattttt agcaaatcat ggtaggtacc
     6241 taatgaagca ttaaatctat cttcaactcc tgaaatttca acactatcaa aacattctat
     6301 ttttttgaaa taatcttctt ttaattgctt aacggttact tttcgatttg ttttgaagag
     6361 taaatcaaca atggctttct tctgttcacc tgaaagaaat gctggttttc gcattccttc
     6421 agtaacatat ttgacctttg tcaattcgtt ataaaccgta aaatactcat aaagcaaact
     6481 atgttttggt agtacttttt catttggaag atttttatca aagtttgtca tgcgttcaat
     6541 aaatgattga gctgaagcac ctttatcgac aacttcttca aaattccatg gggtaattgt
     6601 ttcttcagac ttccgagtca tccatgcaaa acgactattg ccacgcgcca atggaccaac
     6661 ataataagga attcgaaaag tcaagatttt ttcaatcttc tcacgattgt cttttaaaaa
     6721 tggataaaag tcttcttgtc ttctcaaaat agcatgcagc tcacccaagt gaatttgatg
     6781 gggaatagag ccgttgtcaa aggtccgttg cttgcgcagc aaatcttcac gatttagttt
     6841 caccaataat tcctcagtac catccatttt ttctaaaatt ggtttgataa atttataaaa
     6901 ttcttcttgg ctagctcccc catcaatata acctgcatat ccgttttttg attgatcaaa
     6961 aaagatttct ttatactttt ctggaagttg ttgtcgaact aaagctttta aaagagtcaa
     7021 gtcttgatga tgttcatcgt agcgtttaat cattgaagct gataggggag ccttagttat
     7081 ttcagtattt actcttagga tatctgaaag taaaatagca tctgataaat tcttagctgc
     7141 caaaaacaaa tcagcatatt gatctccaat ttgcgccaat aaattatcta aatcatcatc
     7201 gtaagtatct tttgaaagct gtaatttagc atcttctgcc aaatcaaaat ttgatttaaa
     7261 attaggggtc aaacccaatg acaaagcaat gagattccca aataagccat ttttcttctc
     7321 accggggagc tgagcaatga gattttctaa tcgtcttgat ttactcaatc gtgcagaaag
     7381 aatcgcttta gcatctactc cacttgcgtt aatagggttt tcttcaaata attgattgta
     7441 ggtttgtacc aactggataa atagtttgtc cacatcacta ttatcaggat ttaaatctcc
     7501 ctcaatcaaa aaatgaccac gaaacttaat catatgcgct aaggccaaat agattaagcg
     7561 caaatccgct ttatcagtag aatctaccaa tttttttcgc agatgataga tagttggata
     7621 tttctcatga taagcaactt catctactat atttccaaaa ataggatgac gttcatgctt
     7681 cttgtcttct tccaccaaaa aagactcttc aagtcgatga aagaaactat catctacttt
     7741 cgccatctca tttgaaaaaa tctcctgtag ataacaaata cgattcttcc gacgtgtata
     7801 ccttctacga gctgtccgtt tgagacgagt cgcttccgct gtctctccac tgtcaaataa
     7861 aagagcccct ataagatttt ttttgatact gtggcggtct gtatttccca gaaccttgaa
     7921 ctttttagac ggaaccttat attcatcagt gatcaccgcc catccgacgc tatttgtgcc
     7981 gatagctaag cctattgagt atttcttatc catttttgcc tcctaaaata aaaagtttaa
     8041 attaaatcca taatgagttt gatgatttca ataatagttt taatgacctc cgaaattagt
     8101 ttaatatgct ttaatttttc tttttcaaaa tatctcttca aaaaatatta cccaatactt
     8161 aataataaat agattataac acaaaattct tttaaaaagt agtttatttt gttatcattc
     8221 tatagtatta agtattgttt tatggctgat aaatttcttt gaatttctcc ttgattattt
     8281 gttataaaag ttataaaata atcttgttgg aaccattcaa aacagcatag caagttaaaa
     8341 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgatact
     8401 tctattctac tctgactgca aaccaaaaaa acaagcgctt tcaaaacgct tgttttatca
     8461 tttttaggga aattaatctc ttaatccttt tatcattcta catttaggcg ctgccatctt
     8521 gctaaaccta ctaagctcca caggatgatt tcgtaatgcg cccaatacgc aaaccgcctc
     8581 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8641 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8701 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8761 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8821 aaaagctggt ctagcttaag agttcaccga caaacaacag ataaaacgaa aggcccagtc
     8881 tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatggggag
     8941 accccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg
     9001 ggaccaccgc gctactgccg ccaggcaaat tctgttttat cagaccgctt ctgcgttctg
     9061 atttaatctg tatcaggctg aaaatcttct ctcatccgcc aaaacagcca agctggagac
     9121 cgtttaaact caatgatgat gatgatgatg gtcgacggcg ctattcagat cctcttctga
     9181 gatgagtttt tgttcgggcc caagcttcaa aaaaagcaca tgggtgatcc tcatgtgcca
     9241 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaaca
     9301 tcctgtccct agtggcccca ctagtattat acctaggact gagctagctg tcaagagctc
     9361 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     9421 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     9481 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     9541 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     9601 taaatcagct cattttttaa ccaataggcc gtactgcgat gagtggcagg gcggggcgta
     9661 atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga
     9721 taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt cggttcaggg
     9781 cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt gactaccgga
     9841 agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg
     9901 gaggtaataa ttgacgatat gatcatttat tctgcctccc agagcctgat aaaaacggtg
     9961 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
    10021 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gaggcggcta cagccgatag
    10081 tctggaacag cgcacttacg ggttgctgcg caacccaagt gctaccggcg cggcagcgtg
    10141 acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa aacacggctc atcgggcatc
    10201 ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt cggtcaaggc tggcaggtct
    10261 ggttccatgc ccggaatgcc gggctggctg ggcggctcct cgccggggcc ggtcggtagt
    10321 tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt cgccatgccc caacagcgat
    10381 tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga acaccgacag gcgcaactgg
    10441 tcgcggggct ggccccacgc cacgcggtca ttgaccacgt aggccgacac ggtgccgggg
    10501 ccgttgagct tcacgacgga gatccagcgc tcggccacca agtccttgac tgcgtattgg
    10561 accgtccgca aagaacgtcc gatgagcttg gaaagtgtct tctggctgac caccacggcg
    10621 ttctggtggc ccatctgcgc cacgaggtga tgcagcagca ttgccgccgt gggtttcctc
    10681 gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca gtgaccgggc
    10741 ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg ccatgcttat ctccatgcgg
    10801 taggggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga
    10861 caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta acctacgcaa
    10921 tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt taagttgttg
    10981 atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa agaagggcac
    11041 ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc
    11101 cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc tgcccccttg
    11161 gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt
//
