LOCUS       sequence-34477-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  TITLE     Barton Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Flag-TRIM24-F869D
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             118..141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgc caccatggca gactacaagg acgacgatga caaggcagac
      121 tacaaggacg acgatgacaa gggtacctct agaagatcta ggacaatgga ggtggcggtg
      181 gagaaggcgg tggcggcggc ggcagcggcc tcggctgcgg cctccggggg gccctcggcg
      241 gcgccgagcg gggagaacga ggccgagagt cggcagggcc cggactcgga gcgcggcggc
      301 gaggcggccc ggctcaacct gttggacact tgcgccgtgt gccaccagaa catccagagc
      361 cgggcgccca agctgctgcc ctgcctgcac tctttctgcc agcgctgcct gcccgcgccc
      421 cagcgctacc tcatgctgcc cgcgcccatg ctgggctcgg ccgagacccc gccacccgtc
      481 cctgcccccg gctcgccggt cagcggctcg tcgccgttcg ccacccaagt tggagtcatt
      541 cgttgcccag tttgcagcca agaatgtgca gagagacaca tcatagataa cttttttgtg
      601 aaggacacta ctgaggttcc cagcagtaca gtagaaaagt caaatcaggt atgtacaagc
      661 tgtgaggaca acgcagaagc caatgggttt tgtgtagagt gtgttgaatg gctctgcaag
      721 acgtgtatc
//
