LOCUS       sequence-34480-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Barton Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Flag-TRIM24-F979A/N980A
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             125..148
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccac catggcagac tacaaggacg acgatgacaa
      121 ggcagactac aaggacgacg atgacaaggg tacctctaga agatctagga caatggaggt
      181 ggcggtggag aaggcggtgg cggcggcggc agcggcctcg gctgcggcct ccggggggcc
      241 ctcggcggcg ccgagcgggg agaacgaggc cgagagtcgg cagggcccgg actcggagcg
      301 cggcggcgag gcggcccggc tcaacctgtt ggacacttgc gccgtgtgcc accagaacat
      361 ccagagccgg gcgcccaagc tgctgccctg cctgcactct ttctgccagc gctgcctgcc
      421 cgcgccccag cgctacctca tgctgcccgc gcccatgctg ggctcggccg agaccccgcc
      481 acccgtccct gcccccggct cgccggtcag cggctcgtcg ccgttcgcca cccaagttgg
      541 agtcattcgt tgcccagttt gcagccaaga atgtgcagag agacacatca tagataactt
      601 ttttgtgaag gacactactg aggttcccag cagtacagta gaaaagtcaa atcaggtatg
      661 tacaagctgt gaggacaacg cagaagccaa tgggttttgt gtagagtgtg ttgaatggct
      721 ctgcaagacg tgtatcagag ctcatcagag ggtaaagttc acaaaagacc acactgtcag
      781 acagaaagag gaagtatctc cagaggcagt tggtgtcacc agccagcgac cagtgttttg
      841 tccttttcat aaaaaggagc
//
